Presence of mycoplasmas in cell cultures in Argentina

The presence of mycoplasmas in cell cultures used for virus propagation in Argentine laboratories, was studied. Samples were obtained from laboratories located in the city of Buenos Aires and provinces of Córdoba and Buenos Aires. The fluorescent stain Hoechst 33258, which specifically binds to DNA,...

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Autores principales:	Coronato, S., Coto, C.
Formato:	JOUR
Materias:	bisbenzimide animal article cell culture culture technique human isolation and purification microbiology Mycoplasmatales staining standard virus culture Animals Bisbenzimide Cells, Cultured Culture Techniques Humans Staining and Labeling Virus Cultivation
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_03257541_v19_n4_p165_Coronato
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_03257541_v19_n4_p165_Coronato
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spelling	todo:paper_03257541_v19_n4_p165_Coronato2023-10-03T15:23:55Z Presence of mycoplasmas in cell cultures in Argentina Coronato, S. Coto, C. bisbenzimide animal article cell culture culture technique human isolation and purification microbiology Mycoplasmatales staining standard virus culture Animals Bisbenzimide Cells, Cultured Culture Techniques Humans Mycoplasmatales Staining and Labeling Virus Cultivation The presence of mycoplasmas in cell cultures used for virus propagation in Argentine laboratories, was studied. Samples were obtained from laboratories located in the city of Buenos Aires and provinces of Córdoba and Buenos Aires. The fluorescent stain Hoechst 33258, which specifically binds to DNA, was used for mycoplasma testing. Thirty three (65%) of 51 analyzed cell cultures (48 established lines and 3 primary cultures), were found contaminated despite that all laboratories were well equipped to work under sterile conditions. The high rate of infection observed let us conclude that the risk to work with mycoplasma contaminated cultures was not properly appreciated. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03257541_v19_n4_p165_Coronato
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	bisbenzimide animal article cell culture culture technique human isolation and purification microbiology Mycoplasmatales staining standard virus culture Animals Bisbenzimide Cells, Cultured Culture Techniques Humans Mycoplasmatales Staining and Labeling Virus Cultivation
spellingShingle	bisbenzimide animal article cell culture culture technique human isolation and purification microbiology Mycoplasmatales staining standard virus culture Animals Bisbenzimide Cells, Cultured Culture Techniques Humans Mycoplasmatales Staining and Labeling Virus Cultivation Coronato, S. Coto, C. Presence of mycoplasmas in cell cultures in Argentina
topic_facet	bisbenzimide animal article cell culture culture technique human isolation and purification microbiology Mycoplasmatales staining standard virus culture Animals Bisbenzimide Cells, Cultured Culture Techniques Humans Mycoplasmatales Staining and Labeling Virus Cultivation
description	The presence of mycoplasmas in cell cultures used for virus propagation in Argentine laboratories, was studied. Samples were obtained from laboratories located in the city of Buenos Aires and provinces of Córdoba and Buenos Aires. The fluorescent stain Hoechst 33258, which specifically binds to DNA, was used for mycoplasma testing. Thirty three (65%) of 51 analyzed cell cultures (48 established lines and 3 primary cultures), were found contaminated despite that all laboratories were well equipped to work under sterile conditions. The high rate of infection observed let us conclude that the risk to work with mycoplasma contaminated cultures was not properly appreciated.
format	JOUR
author	Coronato, S. Coto, C.
author_facet	Coronato, S. Coto, C.
author_sort	Coronato, S.
title	Presence of mycoplasmas in cell cultures in Argentina
title_short	Presence of mycoplasmas in cell cultures in Argentina
title_full	Presence of mycoplasmas in cell cultures in Argentina
title_fullStr	Presence of mycoplasmas in cell cultures in Argentina
title_full_unstemmed	Presence of mycoplasmas in cell cultures in Argentina
title_sort	presence of mycoplasmas in cell cultures in argentina
url	http://hdl.handle.net/20.500.12110/paper_03257541_v19_n4_p165_Coronato
work_keys_str_mv	AT coronatos presenceofmycoplasmasincellculturesinargentina AT cotoc presenceofmycoplasmasincellculturesinargentina
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Presence of mycoplasmas in cell cultures in Argentina

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