Innate immune response between environmental factors and abortion: Lipopolysaccharide tolerization model during gestation

The exacerbated local inflammatory response that triggers immunological abortion on CBA/j x DBA/2 murine model depends on environmental factors such as LPS. Since deficient Tlr4 mice are resistant to LPS-induced abortion, the effect of LPS tolerization on the prevention of fetal losses, as well as o...

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Autores principales:	Dubinsky, V., Junovich, G., Gutiérrez, G.
Formato:	JOUR
Materias:	Abortion Cytokines Innate immune response Lipopolysaccharide Tolerization Murinae
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_03252957_v40_n1_p71_Dubinsky
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_03252957_v40_n1_p71_Dubinsky
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spelling	todo:paper_03252957_v40_n1_p71_Dubinsky2023-10-03T15:23:38Z Innate immune response between environmental factors and abortion: Lipopolysaccharide tolerization model during gestation Dubinsky, V. Junovich, G. Gutiérrez, G. Abortion Cytokines Innate immune response Lipopolysaccharide Tolerization Murinae The exacerbated local inflammatory response that triggers immunological abortion on CBA/j x DBA/2 murine model depends on environmental factors such as LPS. Since deficient Tlr4 mice are resistant to LPS-induced abortion, the effect of LPS tolerization on the prevention of fetal losses, as well as on the synthesis of placental cytokines on CBA/j x DBA/2 model has been studied. Low doses of LPS were inoculated during pre and post-implantatory stages on CBA/j pregnant mice. In order to investigate tolerization effect, abortogenic doses of LPS were added to placental and splenocyte cul-Results obtained with post-implantatory inoculation of 1μg/day LPS showed a decrease in the abortion rate as well as an IL-6 hyporesponsive to LPS booster, both in placenta and splenocyte cultures. However, stimulation with abortogenic dose of LPS modified TNF-α levels by an increase in placenta but a decrease in splenocyte culture supernatants. Considering these results, it can be concluded that LPS tolerance induction depends on the organ as well as on the cytokine studied. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03252957_v40_n1_p71_Dubinsky
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Abortion Cytokines Innate immune response Lipopolysaccharide Tolerization Murinae
spellingShingle	Abortion Cytokines Innate immune response Lipopolysaccharide Tolerization Murinae Dubinsky, V. Junovich, G. Gutiérrez, G. Innate immune response between environmental factors and abortion: Lipopolysaccharide tolerization model during gestation
topic_facet	Abortion Cytokines Innate immune response Lipopolysaccharide Tolerization Murinae
description	The exacerbated local inflammatory response that triggers immunological abortion on CBA/j x DBA/2 murine model depends on environmental factors such as LPS. Since deficient Tlr4 mice are resistant to LPS-induced abortion, the effect of LPS tolerization on the prevention of fetal losses, as well as on the synthesis of placental cytokines on CBA/j x DBA/2 model has been studied. Low doses of LPS were inoculated during pre and post-implantatory stages on CBA/j pregnant mice. In order to investigate tolerization effect, abortogenic doses of LPS were added to placental and splenocyte cul-Results obtained with post-implantatory inoculation of 1μg/day LPS showed a decrease in the abortion rate as well as an IL-6 hyporesponsive to LPS booster, both in placenta and splenocyte cultures. However, stimulation with abortogenic dose of LPS modified TNF-α levels by an increase in placenta but a decrease in splenocyte culture supernatants. Considering these results, it can be concluded that LPS tolerance induction depends on the organ as well as on the cytokine studied.
format	JOUR
author	Dubinsky, V. Junovich, G. Gutiérrez, G.
author_facet	Dubinsky, V. Junovich, G. Gutiérrez, G.
author_sort	Dubinsky, V.
title	Innate immune response between environmental factors and abortion: Lipopolysaccharide tolerization model during gestation
title_short	Innate immune response between environmental factors and abortion: Lipopolysaccharide tolerization model during gestation
title_full	Innate immune response between environmental factors and abortion: Lipopolysaccharide tolerization model during gestation
title_fullStr	Innate immune response between environmental factors and abortion: Lipopolysaccharide tolerization model during gestation
title_full_unstemmed	Innate immune response between environmental factors and abortion: Lipopolysaccharide tolerization model during gestation
title_sort	innate immune response between environmental factors and abortion: lipopolysaccharide tolerization model during gestation
url	http://hdl.handle.net/20.500.12110/paper_03252957_v40_n1_p71_Dubinsky
work_keys_str_mv	AT dubinskyv innateimmuneresponsebetweenenvironmentalfactorsandabortionlipopolysaccharidetolerizationmodelduringgestation AT junovichg innateimmuneresponsebetweenenvironmentalfactorsandabortionlipopolysaccharidetolerizationmodelduringgestation AT gutierrezg innateimmuneresponsebetweenenvironmentalfactorsandabortionlipopolysaccharidetolerizationmodelduringgestation
_version_	1782030430020567040

Innate immune response between environmental factors and abortion: Lipopolysaccharide tolerization model during gestation

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