Caracterización de fracciones mayoritarias del plasma seminal humano. Estudio comparative entre individuos normospérmicos, azoospérmicos, necrozoospérmicos, oligozoospérmicos y vasectomizados

Normospermic and pathological human seminal plasma (PSH) was analysed by affinity chromatography in Concanavalin-A Sepharose. Two main fractions were obtained, F1 and F2, in variable proportions and with different glycan content according with the type of pathology. Characterization of the oligosacc...

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Autores principales:	Tortorella, H., Konrath, R.O., Brandelli, A., Furtado, J.L.F., Mazzini, M.N.
Formato:	JOUR
Materias:	Affinity chromatography Carbohydrate Glycoprotein Lectin Semen
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_03252957_v30_n1_p49_Tortorella
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_03252957_v30_n1_p49_Tortorella
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spelling	todo:paper_03252957_v30_n1_p49_Tortorella2023-10-03T15:23:31Z Caracterización de fracciones mayoritarias del plasma seminal humano. Estudio comparative entre individuos normospérmicos, azoospérmicos, necrozoospérmicos, oligozoospérmicos y vasectomizados Tortorella, H. Konrath, R.O. Brandelli, A. Furtado, J.L.F. Mazzini, M.N. Affinity chromatography Carbohydrate Glycoprotein Lectin Semen Normospermic and pathological human seminal plasma (PSH) was analysed by affinity chromatography in Concanavalin-A Sepharose. Two main fractions were obtained, F1 and F2, in variable proportions and with different glycan content according with the type of pathology. Characterization of the oligosaccharide chains of F1 and F2 showed that galactose is always the main component together with fucose and variable amounts of mannose, N-acetylglucosamine and N-acetylgalactosamine. These fractions showed differences in the primary structure of glycoprotein glycans (N- and O-glycosidic-linked), according to the pathology. All the oligosaccharide chains in F1 correspond to mucin type glycoproteins (O-glycosidic type), whereas in F2 the molar ratio O-/N- is 5.3/1. F1 from azoospermic and oligozoospermic are nearly all of the O-glycosid type while in vasectomised the oligosaccharide chains differ from the other ones by a molar ratio O-/N- of 1.7/1. F2 present both O- and N- oligosaccharide chains in different amounts, with the only exception of vasectomised which belongs to O-glycosid type. The results obtained suggest that the composition of major PSH fractions from donors with different pathologies is characterized by marked structural differences on the glycan and the protein moiety. Fil:Tortorella, H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Brandelli, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Mazzini, M.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03252957_v30_n1_p49_Tortorella
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Affinity chromatography Carbohydrate Glycoprotein Lectin Semen
spellingShingle	Affinity chromatography Carbohydrate Glycoprotein Lectin Semen Tortorella, H. Konrath, R.O. Brandelli, A. Furtado, J.L.F. Mazzini, M.N. Caracterización de fracciones mayoritarias del plasma seminal humano. Estudio comparative entre individuos normospérmicos, azoospérmicos, necrozoospérmicos, oligozoospérmicos y vasectomizados
topic_facet	Affinity chromatography Carbohydrate Glycoprotein Lectin Semen
description	Normospermic and pathological human seminal plasma (PSH) was analysed by affinity chromatography in Concanavalin-A Sepharose. Two main fractions were obtained, F1 and F2, in variable proportions and with different glycan content according with the type of pathology. Characterization of the oligosaccharide chains of F1 and F2 showed that galactose is always the main component together with fucose and variable amounts of mannose, N-acetylglucosamine and N-acetylgalactosamine. These fractions showed differences in the primary structure of glycoprotein glycans (N- and O-glycosidic-linked), according to the pathology. All the oligosaccharide chains in F1 correspond to mucin type glycoproteins (O-glycosidic type), whereas in F2 the molar ratio O-/N- is 5.3/1. F1 from azoospermic and oligozoospermic are nearly all of the O-glycosid type while in vasectomised the oligosaccharide chains differ from the other ones by a molar ratio O-/N- of 1.7/1. F2 present both O- and N- oligosaccharide chains in different amounts, with the only exception of vasectomised which belongs to O-glycosid type. The results obtained suggest that the composition of major PSH fractions from donors with different pathologies is characterized by marked structural differences on the glycan and the protein moiety.
format	JOUR
author	Tortorella, H. Konrath, R.O. Brandelli, A. Furtado, J.L.F. Mazzini, M.N.
author_facet	Tortorella, H. Konrath, R.O. Brandelli, A. Furtado, J.L.F. Mazzini, M.N.
author_sort	Tortorella, H.
title	Caracterización de fracciones mayoritarias del plasma seminal humano. Estudio comparative entre individuos normospérmicos, azoospérmicos, necrozoospérmicos, oligozoospérmicos y vasectomizados
title_short	Caracterización de fracciones mayoritarias del plasma seminal humano. Estudio comparative entre individuos normospérmicos, azoospérmicos, necrozoospérmicos, oligozoospérmicos y vasectomizados
title_full	Caracterización de fracciones mayoritarias del plasma seminal humano. Estudio comparative entre individuos normospérmicos, azoospérmicos, necrozoospérmicos, oligozoospérmicos y vasectomizados
title_fullStr	Caracterización de fracciones mayoritarias del plasma seminal humano. Estudio comparative entre individuos normospérmicos, azoospérmicos, necrozoospérmicos, oligozoospérmicos y vasectomizados
title_full_unstemmed	Caracterización de fracciones mayoritarias del plasma seminal humano. Estudio comparative entre individuos normospérmicos, azoospérmicos, necrozoospérmicos, oligozoospérmicos y vasectomizados
title_sort	caracterización de fracciones mayoritarias del plasma seminal humano. estudio comparative entre individuos normospérmicos, azoospérmicos, necrozoospérmicos, oligozoospérmicos y vasectomizados
url	http://hdl.handle.net/20.500.12110/paper_03252957_v30_n1_p49_Tortorella
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Caracterización de fracciones mayoritarias del plasma seminal humano. Estudio comparative entre individuos normospérmicos, azoospérmicos, necrozoospérmicos, oligozoospérmicos y vasectomizados

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