In situ measurement of cAMP related enzymes in the dimorphic fungus Mucor rouxii

Yeast cells of the dimorphic fungus Mucor rouxii have been permeabilized by treatment with toluene:ethanol. The permeabilization allowed the in situ measurement of pyruvate kinase, cAMP phosphodiesterase and adenylate cyclase activities. Using a small peptide as substrate, cAMP dependent protein kin...

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Detalles Bibliográficos
Autores principales: Maggese, M.C., Galvagno, M.A., Cantore, M.L., Passeron, S.
Formato: JOUR
Materias:
3',5' cyclic nucleotide phosphodiesterase
adenylate cyclase
cyclic AMP
protein kinase
pyruvate kinase
toluene
article
comparative study
metabolism
Mucor
permeability
3',5'-Cyclic-Nucleotide Phosphodiesterase
Adenylate Cyclase
Comparative Study
Cyclic AMP
Permeability
Protein Kinases
Pyruvate Kinase
Support, Non-U.S. Gov't
Toluene
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_03091651_v6_n12_p1101_Maggese
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_03091651_v6_n12_p1101_Maggese
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spelling todo:paper_03091651_v6_n12_p1101_Maggese2023-10-03T15:23:01Z In situ measurement of cAMP related enzymes in the dimorphic fungus Mucor rouxii Maggese, M.C. Galvagno, M.A. Cantore, M.L. Passeron, S. 3',5' cyclic nucleotide phosphodiesterase adenylate cyclase cyclic AMP protein kinase pyruvate kinase toluene article comparative study metabolism Mucor permeability 3',5'-Cyclic-Nucleotide Phosphodiesterase Adenylate Cyclase Comparative Study Cyclic AMP Mucor Permeability Protein Kinases Pyruvate Kinase Support, Non-U.S. Gov't Toluene Yeast cells of the dimorphic fungus Mucor rouxii have been permeabilized by treatment with toluene:ethanol. The permeabilization allowed the in situ measurement of pyruvate kinase, cAMP phosphodiesterase and adenylate cyclase activities. Using a small peptide as substrate, cAMP dependent protein kinase activity could also be measured. Permeabilized cells showed higher cAMP phosphodiesterase and adenylate cyclase activities than cellular homogenates. The main catalytic properties of the enzymes were similar to that previously found in in vitro studies. © 1982. Fil:Maggese, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Galvagno, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cantore, M.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03091651_v6_n12_p1101_Maggese
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic 3',5' cyclic nucleotide phosphodiesterase
adenylate cyclase
cyclic AMP
protein kinase
pyruvate kinase
toluene
article
comparative study
metabolism
Mucor
permeability
3',5'-Cyclic-Nucleotide Phosphodiesterase
Adenylate Cyclase
Comparative Study
Cyclic AMP
Mucor
Permeability
Protein Kinases
Pyruvate Kinase
Support, Non-U.S. Gov't
Toluene
spellingShingle 3',5' cyclic nucleotide phosphodiesterase
adenylate cyclase
cyclic AMP
protein kinase
pyruvate kinase
toluene
article
comparative study
metabolism
Mucor
permeability
3',5'-Cyclic-Nucleotide Phosphodiesterase
Adenylate Cyclase
Comparative Study
Cyclic AMP
Mucor
Permeability
Protein Kinases
Pyruvate Kinase
Support, Non-U.S. Gov't
Toluene
Maggese, M.C.
Galvagno, M.A.
Cantore, M.L.
Passeron, S.
In situ measurement of cAMP related enzymes in the dimorphic fungus Mucor rouxii
topic_facet 3',5' cyclic nucleotide phosphodiesterase
adenylate cyclase
cyclic AMP
protein kinase
pyruvate kinase
toluene
article
comparative study
metabolism
Mucor
permeability
3',5'-Cyclic-Nucleotide Phosphodiesterase
Adenylate Cyclase
Comparative Study
Cyclic AMP
Mucor
Permeability
Protein Kinases
Pyruvate Kinase
Support, Non-U.S. Gov't
Toluene
description Yeast cells of the dimorphic fungus Mucor rouxii have been permeabilized by treatment with toluene:ethanol. The permeabilization allowed the in situ measurement of pyruvate kinase, cAMP phosphodiesterase and adenylate cyclase activities. Using a small peptide as substrate, cAMP dependent protein kinase activity could also be measured. Permeabilized cells showed higher cAMP phosphodiesterase and adenylate cyclase activities than cellular homogenates. The main catalytic properties of the enzymes were similar to that previously found in in vitro studies. © 1982.
format JOUR
author Maggese, M.C.
Galvagno, M.A.
Cantore, M.L.
Passeron, S.
author_facet Maggese, M.C.
Galvagno, M.A.
Cantore, M.L.
Passeron, S.
author_sort Maggese, M.C.
title In situ measurement of cAMP related enzymes in the dimorphic fungus Mucor rouxii
title_short In situ measurement of cAMP related enzymes in the dimorphic fungus Mucor rouxii
title_full In situ measurement of cAMP related enzymes in the dimorphic fungus Mucor rouxii
title_fullStr In situ measurement of cAMP related enzymes in the dimorphic fungus Mucor rouxii
title_full_unstemmed In situ measurement of cAMP related enzymes in the dimorphic fungus Mucor rouxii
title_sort in situ measurement of camp related enzymes in the dimorphic fungus mucor rouxii
url http://hdl.handle.net/20.500.12110/paper_03091651_v6_n12_p1101_Maggese
work_keys_str_mv AT maggesemc insitumeasurementofcamprelatedenzymesinthedimorphicfungusmucorrouxii
AT galvagnoma insitumeasurementofcamprelatedenzymesinthedimorphicfungusmucorrouxii
AT cantoreml insitumeasurementofcamprelatedenzymesinthedimorphicfungusmucorrouxii
AT passerons insitumeasurementofcamprelatedenzymesinthedimorphicfungusmucorrouxii
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