Optimization of β-cyclodextrin-based extraction of antioxidant and anti-browning activities from thyme leaves by response surface methodology
Thyme (Thymus vulgaris) has been demonstrated to extend the shelf-life of food products, being also a potential source of bioactive compounds. The aim of this research was to optimize the ultrasound assisted extraction employing β-cyclodextrin aqueous solutions as no-contaminant technology and Respo...
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todo:paper_03088146_v265_n_p86_Favre2023-10-03T15:22:56Z Optimization of β-cyclodextrin-based extraction of antioxidant and anti-browning activities from thyme leaves by response surface methodology Favre, L.C. dos Santos, C. López-Fernández, M.P. Mazzobre, M.F. Buera, M.D.P. 2,2-Diphenyl-1-picrylhydrazyl radical (DPPH[rad]) (PubChem CDI: 2735032) 2,4,6-Tripyridyl-s-triazine (TPTZ) (PubChem CID: 77258) Anti-glycation Antioxidant activity Bovine serum albumin (BSA) (PubChem CID: 16132389) Ferric chloride hexahydrate (PubChem CID: 6093258) Gallic acid (PubChem CID: 370) Glucose (PubChem CID: 5793) Maillard Phosphate buffered saline (PBS) (PubChem CID: 24978514) Sodium carbonate trihydrate (PubChem CID: 10340) Thyme Ultrasonic β-Cyclodextrin β-Cyclodextrin (β-CD) (PubChem CID: 444041) Body fluids Chlorination Chlorine compounds Cyclodextrins Extraction Food products Glycosylation Sodium bicarbonate Sodium Carbonate Solutions Surface properties Ultrasonics Anti-oxidant activities Glycation Maillard Pubchem Thyme Antioxidants beta cyclodextrin polyphenol derivative Thymus vulgaris extract antioxidant activity aqueous solution Article chemical reaction kinetics controlled study DPPH radical scavenging assay ferric reducing antioxidant power assay glycation green chemistry plant leaf process optimization response surface method temperature thyme ultrasound assisted extraction ultraviolet visible spectroscopy Thyme (Thymus vulgaris) has been demonstrated to extend the shelf-life of food products, being also a potential source of bioactive compounds. The aim of this research was to optimize the ultrasound assisted extraction employing β-cyclodextrin aqueous solutions as no-contaminant technology and Response Surface Methodology to obtain thyme extracts with the maximum antioxidant capacity. The optimal extraction conditions were: a solution of β-ciclodextrin 15 mM, an ultrasonic treatment time of 5.9 min at a temperature of 36.6 °C. They resulted in an extract with a polyphenolic content of 189.3 mg GAE/mL, an antioxidant activity (DPPH[rad]) of 14.8 mg GAE/mL, and ferric reducing/antioxidant power (FRAP) of 3.3 mg GAE/mL. Interestingly, the extract demonstrated to inhibit the production of Maillard browning products and can be considered a potential antiglycant agent. The obtained data is important for developing eco-friendly technologies in order to obtain natural antioxidant extracts with a potential inhibitory capacity of Maillard glycation reaction. © 2018 Elsevier Ltd JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03088146_v265_n_p86_Favre |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
2,2-Diphenyl-1-picrylhydrazyl radical (DPPH[rad]) (PubChem CDI: 2735032) 2,4,6-Tripyridyl-s-triazine (TPTZ) (PubChem CID: 77258) Anti-glycation Antioxidant activity Bovine serum albumin (BSA) (PubChem CID: 16132389) Ferric chloride hexahydrate (PubChem CID: 6093258) Gallic acid (PubChem CID: 370) Glucose (PubChem CID: 5793) Maillard Phosphate buffered saline (PBS) (PubChem CID: 24978514) Sodium carbonate trihydrate (PubChem CID: 10340) Thyme Ultrasonic β-Cyclodextrin β-Cyclodextrin (β-CD) (PubChem CID: 444041) Body fluids Chlorination Chlorine compounds Cyclodextrins Extraction Food products Glycosylation Sodium bicarbonate Sodium Carbonate Solutions Surface properties Ultrasonics Anti-oxidant activities Glycation Maillard Pubchem Thyme Antioxidants beta cyclodextrin polyphenol derivative Thymus vulgaris extract antioxidant activity aqueous solution Article chemical reaction kinetics controlled study DPPH radical scavenging assay ferric reducing antioxidant power assay glycation green chemistry plant leaf process optimization response surface method temperature thyme ultrasound assisted extraction ultraviolet visible spectroscopy |
| spellingShingle |
2,2-Diphenyl-1-picrylhydrazyl radical (DPPH[rad]) (PubChem CDI: 2735032) 2,4,6-Tripyridyl-s-triazine (TPTZ) (PubChem CID: 77258) Anti-glycation Antioxidant activity Bovine serum albumin (BSA) (PubChem CID: 16132389) Ferric chloride hexahydrate (PubChem CID: 6093258) Gallic acid (PubChem CID: 370) Glucose (PubChem CID: 5793) Maillard Phosphate buffered saline (PBS) (PubChem CID: 24978514) Sodium carbonate trihydrate (PubChem CID: 10340) Thyme Ultrasonic β-Cyclodextrin β-Cyclodextrin (β-CD) (PubChem CID: 444041) Body fluids Chlorination Chlorine compounds Cyclodextrins Extraction Food products Glycosylation Sodium bicarbonate Sodium Carbonate Solutions Surface properties Ultrasonics Anti-oxidant activities Glycation Maillard Pubchem Thyme Antioxidants beta cyclodextrin polyphenol derivative Thymus vulgaris extract antioxidant activity aqueous solution Article chemical reaction kinetics controlled study DPPH radical scavenging assay ferric reducing antioxidant power assay glycation green chemistry plant leaf process optimization response surface method temperature thyme ultrasound assisted extraction ultraviolet visible spectroscopy Favre, L.C. dos Santos, C. López-Fernández, M.P. Mazzobre, M.F. Buera, M.D.P. Optimization of β-cyclodextrin-based extraction of antioxidant and anti-browning activities from thyme leaves by response surface methodology |
| topic_facet |
2,2-Diphenyl-1-picrylhydrazyl radical (DPPH[rad]) (PubChem CDI: 2735032) 2,4,6-Tripyridyl-s-triazine (TPTZ) (PubChem CID: 77258) Anti-glycation Antioxidant activity Bovine serum albumin (BSA) (PubChem CID: 16132389) Ferric chloride hexahydrate (PubChem CID: 6093258) Gallic acid (PubChem CID: 370) Glucose (PubChem CID: 5793) Maillard Phosphate buffered saline (PBS) (PubChem CID: 24978514) Sodium carbonate trihydrate (PubChem CID: 10340) Thyme Ultrasonic β-Cyclodextrin β-Cyclodextrin (β-CD) (PubChem CID: 444041) Body fluids Chlorination Chlorine compounds Cyclodextrins Extraction Food products Glycosylation Sodium bicarbonate Sodium Carbonate Solutions Surface properties Ultrasonics Anti-oxidant activities Glycation Maillard Pubchem Thyme Antioxidants beta cyclodextrin polyphenol derivative Thymus vulgaris extract antioxidant activity aqueous solution Article chemical reaction kinetics controlled study DPPH radical scavenging assay ferric reducing antioxidant power assay glycation green chemistry plant leaf process optimization response surface method temperature thyme ultrasound assisted extraction ultraviolet visible spectroscopy |
| description |
Thyme (Thymus vulgaris) has been demonstrated to extend the shelf-life of food products, being also a potential source of bioactive compounds. The aim of this research was to optimize the ultrasound assisted extraction employing β-cyclodextrin aqueous solutions as no-contaminant technology and Response Surface Methodology to obtain thyme extracts with the maximum antioxidant capacity. The optimal extraction conditions were: a solution of β-ciclodextrin 15 mM, an ultrasonic treatment time of 5.9 min at a temperature of 36.6 °C. They resulted in an extract with a polyphenolic content of 189.3 mg GAE/mL, an antioxidant activity (DPPH[rad]) of 14.8 mg GAE/mL, and ferric reducing/antioxidant power (FRAP) of 3.3 mg GAE/mL. Interestingly, the extract demonstrated to inhibit the production of Maillard browning products and can be considered a potential antiglycant agent. The obtained data is important for developing eco-friendly technologies in order to obtain natural antioxidant extracts with a potential inhibitory capacity of Maillard glycation reaction. © 2018 Elsevier Ltd |
| format |
JOUR |
| author |
Favre, L.C. dos Santos, C. López-Fernández, M.P. Mazzobre, M.F. Buera, M.D.P. |
| author_facet |
Favre, L.C. dos Santos, C. López-Fernández, M.P. Mazzobre, M.F. Buera, M.D.P. |
| author_sort |
Favre, L.C. |
| title |
Optimization of β-cyclodextrin-based extraction of antioxidant and anti-browning activities from thyme leaves by response surface methodology |
| title_short |
Optimization of β-cyclodextrin-based extraction of antioxidant and anti-browning activities from thyme leaves by response surface methodology |
| title_full |
Optimization of β-cyclodextrin-based extraction of antioxidant and anti-browning activities from thyme leaves by response surface methodology |
| title_fullStr |
Optimization of β-cyclodextrin-based extraction of antioxidant and anti-browning activities from thyme leaves by response surface methodology |
| title_full_unstemmed |
Optimization of β-cyclodextrin-based extraction of antioxidant and anti-browning activities from thyme leaves by response surface methodology |
| title_sort |
optimization of β-cyclodextrin-based extraction of antioxidant and anti-browning activities from thyme leaves by response surface methodology |
| url |
http://hdl.handle.net/20.500.12110/paper_03088146_v265_n_p86_Favre |
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