Food odor, visual danger stimulus, and retrieval of an aversive memory trigger heat shock protein HSP70 expression in the olfactory lobe of the crab Chasmagnathus granulatus
Although some of the neuronal substrates that support memory process have been shown in optic ganglia, the brain areas activated by memory process are still unknown in crustaceans. Heat shock proteins (HSPs) are synthesized in the CNS not only in response to traumas but also after changes in metabol...
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todo:paper_03064522_v201_n_p239_Frenkel2023-10-03T15:22:25Z Food odor, visual danger stimulus, and retrieval of an aversive memory trigger heat shock protein HSP70 expression in the olfactory lobe of the crab Chasmagnathus granulatus Frenkel, L. Dimant, B. Suárez, L.D. Portiansky, E.L. Delorenzi, A. Arousal Crustaceans Heat shock proteins Memory Olfactory lobes Visual stimulus heat shock protein 70 animal experiment animal tissue arousal article aversive memory Chasmagnathus granulatus controlled study crab defensive behavior food odor glomerulus habituation immunohistochemistry long term memory memory morphometrics nonhuman olfactory bulb organoleptic property priority journal protein analysis protein expression recall smelling visual stimulation Animals Brachyura Brain Mapping Escape Reaction Female Food Gene Expression Regulation Habituation, Psychophysiologic HSP70 Heat-Shock Proteins Mental Recall Odors Olfactory Pathways Photic Stimulation Synapsins Time Factors Although some of the neuronal substrates that support memory process have been shown in optic ganglia, the brain areas activated by memory process are still unknown in crustaceans. Heat shock proteins (HSPs) are synthesized in the CNS not only in response to traumas but also after changes in metabolic activity triggered by the processing of different types of sensory information. Indeed, the expression of citosolic/nuclear forms of HSP70 (HSC/HSP70) has been repeatedly used as a marker for increases in neural metabolic activity in several processes, including psychophysiological stress, fear conditioning, and spatial learning in vertebrates. Previously, we have shown that, in the crab Chasmagnathus, two different environmental challenges, water deprivation and heat shock, trigger a rise in the number of glomeruli of the olfactory lobes (OLs) expressing HSC/HSP70. In this study, we initially performed a morphometric analysis and identified a total of 154 glomeruli in each OL of Chasmagnathus. Here, we found that crabs exposed to food odor stimuli also showed a significant rise in the number of olfactory glomeruli expressing HSC/HSP70. In the crab Chasmagnathus, a powerful memory paradigm based on a change in its defensive strategy against a visual danger stimulus (VDS) has been extensively studied. Remarkably, the iterative presentation of a VDS caused an increase as well. This increase was triggered in animals visually stimulated using protocols that either build up a long-term memory or generate only short-term habituation. Besides, memory reactivation was sufficient to trigger the increase in HSC/HSP70 expression in the OL. Present and previous results strongly suggest that, directly or indirectly, an increase in arousal is a sufficient condition to bring about an increase in HSC/HSP70 expression in the OL of Chasmagnathus. © 2011 IBRO. Fil:Frenkel, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Dimant, B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Suárez, L.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Delorenzi, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03064522_v201_n_p239_Frenkel |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Arousal Crustaceans Heat shock proteins Memory Olfactory lobes Visual stimulus heat shock protein 70 animal experiment animal tissue arousal article aversive memory Chasmagnathus granulatus controlled study crab defensive behavior food odor glomerulus habituation immunohistochemistry long term memory memory morphometrics nonhuman olfactory bulb organoleptic property priority journal protein analysis protein expression recall smelling visual stimulation Animals Brachyura Brain Mapping Escape Reaction Female Food Gene Expression Regulation Habituation, Psychophysiologic HSP70 Heat-Shock Proteins Mental Recall Odors Olfactory Pathways Photic Stimulation Synapsins Time Factors |
| spellingShingle |
Arousal Crustaceans Heat shock proteins Memory Olfactory lobes Visual stimulus heat shock protein 70 animal experiment animal tissue arousal article aversive memory Chasmagnathus granulatus controlled study crab defensive behavior food odor glomerulus habituation immunohistochemistry long term memory memory morphometrics nonhuman olfactory bulb organoleptic property priority journal protein analysis protein expression recall smelling visual stimulation Animals Brachyura Brain Mapping Escape Reaction Female Food Gene Expression Regulation Habituation, Psychophysiologic HSP70 Heat-Shock Proteins Mental Recall Odors Olfactory Pathways Photic Stimulation Synapsins Time Factors Frenkel, L. Dimant, B. Suárez, L.D. Portiansky, E.L. Delorenzi, A. Food odor, visual danger stimulus, and retrieval of an aversive memory trigger heat shock protein HSP70 expression in the olfactory lobe of the crab Chasmagnathus granulatus |
| topic_facet |
Arousal Crustaceans Heat shock proteins Memory Olfactory lobes Visual stimulus heat shock protein 70 animal experiment animal tissue arousal article aversive memory Chasmagnathus granulatus controlled study crab defensive behavior food odor glomerulus habituation immunohistochemistry long term memory memory morphometrics nonhuman olfactory bulb organoleptic property priority journal protein analysis protein expression recall smelling visual stimulation Animals Brachyura Brain Mapping Escape Reaction Female Food Gene Expression Regulation Habituation, Psychophysiologic HSP70 Heat-Shock Proteins Mental Recall Odors Olfactory Pathways Photic Stimulation Synapsins Time Factors |
| description |
Although some of the neuronal substrates that support memory process have been shown in optic ganglia, the brain areas activated by memory process are still unknown in crustaceans. Heat shock proteins (HSPs) are synthesized in the CNS not only in response to traumas but also after changes in metabolic activity triggered by the processing of different types of sensory information. Indeed, the expression of citosolic/nuclear forms of HSP70 (HSC/HSP70) has been repeatedly used as a marker for increases in neural metabolic activity in several processes, including psychophysiological stress, fear conditioning, and spatial learning in vertebrates. Previously, we have shown that, in the crab Chasmagnathus, two different environmental challenges, water deprivation and heat shock, trigger a rise in the number of glomeruli of the olfactory lobes (OLs) expressing HSC/HSP70. In this study, we initially performed a morphometric analysis and identified a total of 154 glomeruli in each OL of Chasmagnathus. Here, we found that crabs exposed to food odor stimuli also showed a significant rise in the number of olfactory glomeruli expressing HSC/HSP70. In the crab Chasmagnathus, a powerful memory paradigm based on a change in its defensive strategy against a visual danger stimulus (VDS) has been extensively studied. Remarkably, the iterative presentation of a VDS caused an increase as well. This increase was triggered in animals visually stimulated using protocols that either build up a long-term memory or generate only short-term habituation. Besides, memory reactivation was sufficient to trigger the increase in HSC/HSP70 expression in the OL. Present and previous results strongly suggest that, directly or indirectly, an increase in arousal is a sufficient condition to bring about an increase in HSC/HSP70 expression in the OL of Chasmagnathus. © 2011 IBRO. |
| format |
JOUR |
| author |
Frenkel, L. Dimant, B. Suárez, L.D. Portiansky, E.L. Delorenzi, A. |
| author_facet |
Frenkel, L. Dimant, B. Suárez, L.D. Portiansky, E.L. Delorenzi, A. |
| author_sort |
Frenkel, L. |
| title |
Food odor, visual danger stimulus, and retrieval of an aversive memory trigger heat shock protein HSP70 expression in the olfactory lobe of the crab Chasmagnathus granulatus |
| title_short |
Food odor, visual danger stimulus, and retrieval of an aversive memory trigger heat shock protein HSP70 expression in the olfactory lobe of the crab Chasmagnathus granulatus |
| title_full |
Food odor, visual danger stimulus, and retrieval of an aversive memory trigger heat shock protein HSP70 expression in the olfactory lobe of the crab Chasmagnathus granulatus |
| title_fullStr |
Food odor, visual danger stimulus, and retrieval of an aversive memory trigger heat shock protein HSP70 expression in the olfactory lobe of the crab Chasmagnathus granulatus |
| title_full_unstemmed |
Food odor, visual danger stimulus, and retrieval of an aversive memory trigger heat shock protein HSP70 expression in the olfactory lobe of the crab Chasmagnathus granulatus |
| title_sort |
food odor, visual danger stimulus, and retrieval of an aversive memory trigger heat shock protein hsp70 expression in the olfactory lobe of the crab chasmagnathus granulatus |
| url |
http://hdl.handle.net/20.500.12110/paper_03064522_v201_n_p239_Frenkel |
| work_keys_str_mv |
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| _version_ |
1807315138307424256 |