P/Q-type calcium channel ablation in a mice glycinergic synapse mediated by multiple types of Ca 2+ channels alters transmitter release and short term plasticity

Ca v2.1 channels (P/Q-type) play a prominent role in controlling neurotransmitter release. Transgenic mice in which the α1A pore-forming subunit of Ca v2.1 channels is ablated (KO) provide a powerful tool to study Ca v2.1 function in synaptic transmission in vivo. Whole-cell patch clamp was used to...

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Autores principales:	Giugovaz-Tropper, B., González-Inchauspe, C., Di Guilmi, M.N., Urbano, F.J., Forsythe, I.D., Uchitel, O.D.
Formato:	JOUR
Materias:	CaV2.1 KO mice Inhibitory glycinergic postsynaptic currents P/Q-type calcium channels Pair pulse facilitation Short term synaptic plasticity 4 aminobutyric acid B receptor baclofen calcium channel L type calcium channel N type calcium channel P type calcium channel Q type glycine receptor guanine nucleotide binding protein animal cell article brain function controlled study electroencephalogram facilitation nerve cell plasticity nerve stimulation neurotransmitter release nonhuman priority journal protein expression protein function short term depression synapse synaptic transmission Animals Brain Stem Calcium Channels Calcium Channels, P-Type Calcium Channels, Q-Type Excitatory Postsynaptic Potentials Glycine Inhibitory Postsynaptic Potentials Mice Mice, Knockout Mice, Transgenic Neuronal Plasticity Neurons Neurotransmitter Agents Organ Culture Techniques Patch-Clamp Techniques Synapses Synaptic Transmission
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_03064522_v192_n_p219_GiugovazTropper
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_03064522_v192_n_p219_GiugovazTropper2023-10-03T15:22:24Z P/Q-type calcium channel ablation in a mice glycinergic synapse mediated by multiple types of Ca 2+ channels alters transmitter release and short term plasticity Giugovaz-Tropper, B. González-Inchauspe, C. Di Guilmi, M.N. Urbano, F.J. Forsythe, I.D. Uchitel, O.D. CaV2.1 KO mice Inhibitory glycinergic postsynaptic currents P/Q-type calcium channels Pair pulse facilitation Short term synaptic plasticity 4 aminobutyric acid B receptor baclofen calcium channel L type calcium channel N type calcium channel P type calcium channel Q type glycine receptor guanine nucleotide binding protein animal cell article brain function controlled study electroencephalogram facilitation nerve cell plasticity nerve stimulation neurotransmitter release nonhuman priority journal protein expression protein function short term depression synapse synaptic transmission Animals Brain Stem Calcium Channels Calcium Channels, P-Type Calcium Channels, Q-Type Excitatory Postsynaptic Potentials Glycine Inhibitory Postsynaptic Potentials Mice Mice, Knockout Mice, Transgenic Neuronal Plasticity Neurons Neurotransmitter Agents Organ Culture Techniques Patch-Clamp Techniques Synapses Synaptic Transmission Ca v2.1 channels (P/Q-type) play a prominent role in controlling neurotransmitter release. Transgenic mice in which the α1A pore-forming subunit of Ca v2.1 channels is ablated (KO) provide a powerful tool to study Ca v2.1 function in synaptic transmission in vivo. Whole-cell patch clamp was used to measure inhibitory glycinergic postsynaptic currents (IPSCs) from the lateral superior olive (LSO). Comparing wild-type (WT) and KO mice, we investigated the relevance of P/Q-type calcium channels at a glycinergic synapse mediated by multiple types of Ca 2+ channels, in opposition to synapses where only this type of Ca 2+ channels are in charge of transmitter release. We found that in KO mice, N-type and L-type Ca 2+ channels control synaptic transmission, resulting in a functional but reduced glycinergic transmitter release. Pair pulse facilitation of synaptic currents is retained in KO mice, even when synaptic transmission is driven by either N or L-type calcium channels alone, in contrast with lack of this phenomenon in other synapses which are exclusively mediated by P/Q-type channels. Thus, pointing a difference between P/Q- and N-type channels present in single or multiple types of calcium channels driven synapses. Significant alterations in short-term synaptic plasticity were observed. KO mice exhibited a stronger short term depression (STD) of IPSCs during repetitive stimulation at high frequency and recovered with a larger time constant compared to WT mice. Finally, transmitter release at the LSO synapse from KO mice was strongly modulated by presynaptic GTP-binding protein-coupled receptor γ-aminobutyric acid type B (GABA B). © 2011. Fil:Giugovaz-Tropper, B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:González-Inchauspe, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Di Guilmi, M.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Uchitel, O.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03064522_v192_n_p219_GiugovazTropper
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	CaV2.1 KO mice Inhibitory glycinergic postsynaptic currents P/Q-type calcium channels Pair pulse facilitation Short term synaptic plasticity 4 aminobutyric acid B receptor baclofen calcium channel L type calcium channel N type calcium channel P type calcium channel Q type glycine receptor guanine nucleotide binding protein animal cell article brain function controlled study electroencephalogram facilitation nerve cell plasticity nerve stimulation neurotransmitter release nonhuman priority journal protein expression protein function short term depression synapse synaptic transmission Animals Brain Stem Calcium Channels Calcium Channels, P-Type Calcium Channels, Q-Type Excitatory Postsynaptic Potentials Glycine Inhibitory Postsynaptic Potentials Mice Mice, Knockout Mice, Transgenic Neuronal Plasticity Neurons Neurotransmitter Agents Organ Culture Techniques Patch-Clamp Techniques Synapses Synaptic Transmission
spellingShingle	CaV2.1 KO mice Inhibitory glycinergic postsynaptic currents P/Q-type calcium channels Pair pulse facilitation Short term synaptic plasticity 4 aminobutyric acid B receptor baclofen calcium channel L type calcium channel N type calcium channel P type calcium channel Q type glycine receptor guanine nucleotide binding protein animal cell article brain function controlled study electroencephalogram facilitation nerve cell plasticity nerve stimulation neurotransmitter release nonhuman priority journal protein expression protein function short term depression synapse synaptic transmission Animals Brain Stem Calcium Channels Calcium Channels, P-Type Calcium Channels, Q-Type Excitatory Postsynaptic Potentials Glycine Inhibitory Postsynaptic Potentials Mice Mice, Knockout Mice, Transgenic Neuronal Plasticity Neurons Neurotransmitter Agents Organ Culture Techniques Patch-Clamp Techniques Synapses Synaptic Transmission Giugovaz-Tropper, B. González-Inchauspe, C. Di Guilmi, M.N. Urbano, F.J. Forsythe, I.D. Uchitel, O.D. P/Q-type calcium channel ablation in a mice glycinergic synapse mediated by multiple types of Ca 2+ channels alters transmitter release and short term plasticity
topic_facet	CaV2.1 KO mice Inhibitory glycinergic postsynaptic currents P/Q-type calcium channels Pair pulse facilitation Short term synaptic plasticity 4 aminobutyric acid B receptor baclofen calcium channel L type calcium channel N type calcium channel P type calcium channel Q type glycine receptor guanine nucleotide binding protein animal cell article brain function controlled study electroencephalogram facilitation nerve cell plasticity nerve stimulation neurotransmitter release nonhuman priority journal protein expression protein function short term depression synapse synaptic transmission Animals Brain Stem Calcium Channels Calcium Channels, P-Type Calcium Channels, Q-Type Excitatory Postsynaptic Potentials Glycine Inhibitory Postsynaptic Potentials Mice Mice, Knockout Mice, Transgenic Neuronal Plasticity Neurons Neurotransmitter Agents Organ Culture Techniques Patch-Clamp Techniques Synapses Synaptic Transmission
description	Ca v2.1 channels (P/Q-type) play a prominent role in controlling neurotransmitter release. Transgenic mice in which the α1A pore-forming subunit of Ca v2.1 channels is ablated (KO) provide a powerful tool to study Ca v2.1 function in synaptic transmission in vivo. Whole-cell patch clamp was used to measure inhibitory glycinergic postsynaptic currents (IPSCs) from the lateral superior olive (LSO). Comparing wild-type (WT) and KO mice, we investigated the relevance of P/Q-type calcium channels at a glycinergic synapse mediated by multiple types of Ca 2+ channels, in opposition to synapses where only this type of Ca 2+ channels are in charge of transmitter release. We found that in KO mice, N-type and L-type Ca 2+ channels control synaptic transmission, resulting in a functional but reduced glycinergic transmitter release. Pair pulse facilitation of synaptic currents is retained in KO mice, even when synaptic transmission is driven by either N or L-type calcium channels alone, in contrast with lack of this phenomenon in other synapses which are exclusively mediated by P/Q-type channels. Thus, pointing a difference between P/Q- and N-type channels present in single or multiple types of calcium channels driven synapses. Significant alterations in short-term synaptic plasticity were observed. KO mice exhibited a stronger short term depression (STD) of IPSCs during repetitive stimulation at high frequency and recovered with a larger time constant compared to WT mice. Finally, transmitter release at the LSO synapse from KO mice was strongly modulated by presynaptic GTP-binding protein-coupled receptor γ-aminobutyric acid type B (GABA B). © 2011.
format	JOUR
author	Giugovaz-Tropper, B. González-Inchauspe, C. Di Guilmi, M.N. Urbano, F.J. Forsythe, I.D. Uchitel, O.D.
author_facet	Giugovaz-Tropper, B. González-Inchauspe, C. Di Guilmi, M.N. Urbano, F.J. Forsythe, I.D. Uchitel, O.D.
author_sort	Giugovaz-Tropper, B.
title	P/Q-type calcium channel ablation in a mice glycinergic synapse mediated by multiple types of Ca 2+ channels alters transmitter release and short term plasticity
title_short	P/Q-type calcium channel ablation in a mice glycinergic synapse mediated by multiple types of Ca 2+ channels alters transmitter release and short term plasticity
title_full	P/Q-type calcium channel ablation in a mice glycinergic synapse mediated by multiple types of Ca 2+ channels alters transmitter release and short term plasticity
title_fullStr	P/Q-type calcium channel ablation in a mice glycinergic synapse mediated by multiple types of Ca 2+ channels alters transmitter release and short term plasticity
title_full_unstemmed	P/Q-type calcium channel ablation in a mice glycinergic synapse mediated by multiple types of Ca 2+ channels alters transmitter release and short term plasticity
title_sort	p/q-type calcium channel ablation in a mice glycinergic synapse mediated by multiple types of ca 2+ channels alters transmitter release and short term plasticity
url	http://hdl.handle.net/20.500.12110/paper_03064522_v192_n_p219_GiugovazTropper
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P/Q-type calcium channel ablation in a mice glycinergic synapse mediated by multiple types of Ca 2+ channels alters transmitter release and short term plasticity

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