The effect of cyanide intoxication on hepatic rhodanese kinetics
1. 1. Results of studies on the kinetics of hepatic rhodanese and the effects of S-adenosyl-l-methionine (SAM) on these kinetic parameters in cyanide-treated and non-treated mice are reported here. 2. 2. The enzyme exhibited typical Michaelis-Menten behaviour with cyanide inhibition at concentration...
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todo:paper_03063623_v21_n2_p219_Buzaleh2023-10-03T15:22:10Z The effect of cyanide intoxication on hepatic rhodanese kinetics Buzaleh, A.M. Vazquez, E.S. Alcira Maria, D.C.B. liver enzyme potassium cyanide s adenosylmethionine animal cell animal experiment article enzyme kinetics intoxication intraperitoneal drug administration mouse nonhuman priority journal xenobiotic metabolism Animal Cyanides Kinetics Liver Mice S-Adenosylmethionine Sulfurtransferases Support, Non-U.S. Gov't Thiosulfate Sulfurtransferase Thiosulfates 1. 1. Results of studies on the kinetics of hepatic rhodanese and the effects of S-adenosyl-l-methionine (SAM) on these kinetic parameters in cyanide-treated and non-treated mice are reported here. 2. 2. The enzyme exhibited typical Michaelis-Menten behaviour with cyanide inhibition at concentrations higher than 50 mM. Km values of 4.74 and 0.85 mM were obtained for thiosulphate and cyanide, respectively, in control mice. 3. 3. These results stress the biological importance of the rhodanese reaction for cyanide detoxification. 4. 4. Km values were not significantly modified when the animals were intoxicated with a lethal (20 mg/kg) or a non-lethal (4 mg/kg) dose of cyanide. 5. 5. SAM treatment either in control or in cyanide-poisoned animals doubled the Km's for cyanide. © 1990. Fil:Buzaleh, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Vazquez, E.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03063623_v21_n2_p219_Buzaleh |
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Universidad de Buenos Aires |
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I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
liver enzyme potassium cyanide s adenosylmethionine animal cell animal experiment article enzyme kinetics intoxication intraperitoneal drug administration mouse nonhuman priority journal xenobiotic metabolism Animal Cyanides Kinetics Liver Mice S-Adenosylmethionine Sulfurtransferases Support, Non-U.S. Gov't Thiosulfate Sulfurtransferase Thiosulfates |
| spellingShingle |
liver enzyme potassium cyanide s adenosylmethionine animal cell animal experiment article enzyme kinetics intoxication intraperitoneal drug administration mouse nonhuman priority journal xenobiotic metabolism Animal Cyanides Kinetics Liver Mice S-Adenosylmethionine Sulfurtransferases Support, Non-U.S. Gov't Thiosulfate Sulfurtransferase Thiosulfates Buzaleh, A.M. Vazquez, E.S. Alcira Maria, D.C.B. The effect of cyanide intoxication on hepatic rhodanese kinetics |
| topic_facet |
liver enzyme potassium cyanide s adenosylmethionine animal cell animal experiment article enzyme kinetics intoxication intraperitoneal drug administration mouse nonhuman priority journal xenobiotic metabolism Animal Cyanides Kinetics Liver Mice S-Adenosylmethionine Sulfurtransferases Support, Non-U.S. Gov't Thiosulfate Sulfurtransferase Thiosulfates |
| description |
1. 1. Results of studies on the kinetics of hepatic rhodanese and the effects of S-adenosyl-l-methionine (SAM) on these kinetic parameters in cyanide-treated and non-treated mice are reported here. 2. 2. The enzyme exhibited typical Michaelis-Menten behaviour with cyanide inhibition at concentrations higher than 50 mM. Km values of 4.74 and 0.85 mM were obtained for thiosulphate and cyanide, respectively, in control mice. 3. 3. These results stress the biological importance of the rhodanese reaction for cyanide detoxification. 4. 4. Km values were not significantly modified when the animals were intoxicated with a lethal (20 mg/kg) or a non-lethal (4 mg/kg) dose of cyanide. 5. 5. SAM treatment either in control or in cyanide-poisoned animals doubled the Km's for cyanide. © 1990. |
| format |
JOUR |
| author |
Buzaleh, A.M. Vazquez, E.S. Alcira Maria, D.C.B. |
| author_facet |
Buzaleh, A.M. Vazquez, E.S. Alcira Maria, D.C.B. |
| author_sort |
Buzaleh, A.M. |
| title |
The effect of cyanide intoxication on hepatic rhodanese kinetics |
| title_short |
The effect of cyanide intoxication on hepatic rhodanese kinetics |
| title_full |
The effect of cyanide intoxication on hepatic rhodanese kinetics |
| title_fullStr |
The effect of cyanide intoxication on hepatic rhodanese kinetics |
| title_full_unstemmed |
The effect of cyanide intoxication on hepatic rhodanese kinetics |
| title_sort |
effect of cyanide intoxication on hepatic rhodanese kinetics |
| url |
http://hdl.handle.net/20.500.12110/paper_03063623_v21_n2_p219_Buzaleh |
| work_keys_str_mv |
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| _version_ |
1807323770971488256 |