Relevance of cytochrome P450 levels in the actions of enflurane and isoflurane in mice: Studies on the haem pathway

1. The effect of the fluorinated ether anaesthetics enflurane and isoflurane in mice on haem metabolism and regulation in different metabolic states, such as depression and induction of cytochrome P450 produced by allylisopropylacetamide (AIA) and imidazole, respectively, was investigated. 2. Mice p...

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Autores principales:	Buzaleh, A.M., Del Carmen Martinez, M., Del Carmen Batlle, A.M.
Formato:	JOUR
Materias:	Allylisopropylacetamide Cytochrome P450 Enflurane Haem metabolism Imidazole Isoflurane Volatile anaesthetics 5 aminolevulinate synthase allylisopropylacetamide cytochrome P450 enflurane heme heme oxygenase imidazole isoflurane porphobilinogen deaminase thiosulfate sulfurtransferase aerobic metabolism animal tissue article controlled study enzyme activity enzyme induction enzyme inhibition heme synthesis liver metabolism male nonhuman rat 5-Aminolevulinate Synthetase Aminolevulinic Acid Ammonia-Lyases Anesthetics, Inhalation Animals Cytochrome P-450 Enzyme System Heme Heme Oxygenase (Decyclizing) Hydroxymethylbilane Synthase Imidazoles Liver Mice Microsomes, Liver
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_03051870_v27_n10_p796_Buzaleh
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_03051870_v27_n10_p796_Buzaleh2023-10-03T15:21:35Z Relevance of cytochrome P450 levels in the actions of enflurane and isoflurane in mice: Studies on the haem pathway Buzaleh, A.M. Del Carmen Martinez, M. Del Carmen Batlle, A.M. Allylisopropylacetamide Cytochrome P450 Enflurane Haem metabolism Imidazole Isoflurane Volatile anaesthetics 5 aminolevulinate synthase allylisopropylacetamide cytochrome P450 enflurane heme heme oxygenase imidazole isoflurane porphobilinogen deaminase thiosulfate sulfurtransferase aerobic metabolism animal tissue article controlled study enzyme activity enzyme induction enzyme inhibition heme synthesis liver metabolism male nonhuman rat 5-Aminolevulinate Synthetase Allylisopropylacetamide Aminolevulinic Acid Ammonia-Lyases Anesthetics, Inhalation Animals Cytochrome P-450 Enzyme System Enflurane Heme Heme Oxygenase (Decyclizing) Hydroxymethylbilane Synthase Imidazoles Isoflurane Liver Mice Microsomes, Liver 1. The effect of the fluorinated ether anaesthetics enflurane and isoflurane in mice on haem metabolism and regulation in different metabolic states, such as depression and induction of cytochrome P450 produced by allylisopropylacetamide (AIA) and imidazole, respectively, was investigated. 2. Mice previously treated with AIA (350 mg/kg, i.p.) or imidazole (400 mg/kg, i.p.) received a single dose (1 mL/kg, i.p.) of enflurane or isoflurane and were killed 20 min after anaesthetic administration. 3. Induction of δ-aminolevulinic acid synthetase (ALA-S) activity was found, as expected, in animals receiving AIA and also in animals treated with AIA plus anaesthesia, but no change in the activity of either porphobilinogenase (PBGase) or porphobilinogen deaminase (PBG-D) activities was detected in these two groups of animals. An additional increase in haem destruction was observed in the AIA plus isoflurane-treated group. When mice were injected with imidazol alone or in combination with the anaesthetics, ALA-S activity was increased 50-90% in all groups, but again no change in PBGase or PBG-D activity was observed. Haem oxygenase was diminished in mice receiving imidazole and anaesthesia. 4. In conclusion, neither enflurane nor isoflurane caused additional disturbances in haem metabolism to those produced by AIA or imidazole alone. Fil:Buzaleh, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Del Carmen Martinez, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Del Carmen Batlle, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03051870_v27_n10_p796_Buzaleh
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Allylisopropylacetamide Cytochrome P450 Enflurane Haem metabolism Imidazole Isoflurane Volatile anaesthetics 5 aminolevulinate synthase allylisopropylacetamide cytochrome P450 enflurane heme heme oxygenase imidazole isoflurane porphobilinogen deaminase thiosulfate sulfurtransferase aerobic metabolism animal tissue article controlled study enzyme activity enzyme induction enzyme inhibition heme synthesis liver metabolism male nonhuman rat 5-Aminolevulinate Synthetase Allylisopropylacetamide Aminolevulinic Acid Ammonia-Lyases Anesthetics, Inhalation Animals Cytochrome P-450 Enzyme System Enflurane Heme Heme Oxygenase (Decyclizing) Hydroxymethylbilane Synthase Imidazoles Isoflurane Liver Mice Microsomes, Liver
spellingShingle	Allylisopropylacetamide Cytochrome P450 Enflurane Haem metabolism Imidazole Isoflurane Volatile anaesthetics 5 aminolevulinate synthase allylisopropylacetamide cytochrome P450 enflurane heme heme oxygenase imidazole isoflurane porphobilinogen deaminase thiosulfate sulfurtransferase aerobic metabolism animal tissue article controlled study enzyme activity enzyme induction enzyme inhibition heme synthesis liver metabolism male nonhuman rat 5-Aminolevulinate Synthetase Allylisopropylacetamide Aminolevulinic Acid Ammonia-Lyases Anesthetics, Inhalation Animals Cytochrome P-450 Enzyme System Enflurane Heme Heme Oxygenase (Decyclizing) Hydroxymethylbilane Synthase Imidazoles Isoflurane Liver Mice Microsomes, Liver Buzaleh, A.M. Del Carmen Martinez, M. Del Carmen Batlle, A.M. Relevance of cytochrome P450 levels in the actions of enflurane and isoflurane in mice: Studies on the haem pathway
topic_facet	Allylisopropylacetamide Cytochrome P450 Enflurane Haem metabolism Imidazole Isoflurane Volatile anaesthetics 5 aminolevulinate synthase allylisopropylacetamide cytochrome P450 enflurane heme heme oxygenase imidazole isoflurane porphobilinogen deaminase thiosulfate sulfurtransferase aerobic metabolism animal tissue article controlled study enzyme activity enzyme induction enzyme inhibition heme synthesis liver metabolism male nonhuman rat 5-Aminolevulinate Synthetase Allylisopropylacetamide Aminolevulinic Acid Ammonia-Lyases Anesthetics, Inhalation Animals Cytochrome P-450 Enzyme System Enflurane Heme Heme Oxygenase (Decyclizing) Hydroxymethylbilane Synthase Imidazoles Isoflurane Liver Mice Microsomes, Liver
description	1. The effect of the fluorinated ether anaesthetics enflurane and isoflurane in mice on haem metabolism and regulation in different metabolic states, such as depression and induction of cytochrome P450 produced by allylisopropylacetamide (AIA) and imidazole, respectively, was investigated. 2. Mice previously treated with AIA (350 mg/kg, i.p.) or imidazole (400 mg/kg, i.p.) received a single dose (1 mL/kg, i.p.) of enflurane or isoflurane and were killed 20 min after anaesthetic administration. 3. Induction of δ-aminolevulinic acid synthetase (ALA-S) activity was found, as expected, in animals receiving AIA and also in animals treated with AIA plus anaesthesia, but no change in the activity of either porphobilinogenase (PBGase) or porphobilinogen deaminase (PBG-D) activities was detected in these two groups of animals. An additional increase in haem destruction was observed in the AIA plus isoflurane-treated group. When mice were injected with imidazol alone or in combination with the anaesthetics, ALA-S activity was increased 50-90% in all groups, but again no change in PBGase or PBG-D activity was observed. Haem oxygenase was diminished in mice receiving imidazole and anaesthesia. 4. In conclusion, neither enflurane nor isoflurane caused additional disturbances in haem metabolism to those produced by AIA or imidazole alone.
format	JOUR
author	Buzaleh, A.M. Del Carmen Martinez, M. Del Carmen Batlle, A.M.
author_facet	Buzaleh, A.M. Del Carmen Martinez, M. Del Carmen Batlle, A.M.
author_sort	Buzaleh, A.M.
title	Relevance of cytochrome P450 levels in the actions of enflurane and isoflurane in mice: Studies on the haem pathway
title_short	Relevance of cytochrome P450 levels in the actions of enflurane and isoflurane in mice: Studies on the haem pathway
title_full	Relevance of cytochrome P450 levels in the actions of enflurane and isoflurane in mice: Studies on the haem pathway
title_fullStr	Relevance of cytochrome P450 levels in the actions of enflurane and isoflurane in mice: Studies on the haem pathway
title_full_unstemmed	Relevance of cytochrome P450 levels in the actions of enflurane and isoflurane in mice: Studies on the haem pathway
title_sort	relevance of cytochrome p450 levels in the actions of enflurane and isoflurane in mice: studies on the haem pathway
url	http://hdl.handle.net/20.500.12110/paper_03051870_v27_n10_p796_Buzaleh
work_keys_str_mv	AT buzaleham relevanceofcytochromep450levelsintheactionsofenfluraneandisofluraneinmicestudiesonthehaempathway AT delcarmenmartinezm relevanceofcytochromep450levelsintheactionsofenfluraneandisofluraneinmicestudiesonthehaempathway AT delcarmenbatlleam relevanceofcytochromep450levelsintheactionsofenfluraneandisofluraneinmicestudiesonthehaempathway
_version_	1782027232166805504

Relevance of cytochrome P450 levels in the actions of enflurane and isoflurane in mice: Studies on the haem pathway

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