A systemic transcriptome analysis reveals the regulation of neural stem cell maintenance by an E2F1-miRNA feedback loop
Stem cell fate decisions are controlled by a molecular network in which transcription factors and miRNAs are of key importance. To systemically investigate their impact on neural stem cell (NSC) maintenance and neuronal commitment, we performed a high-throughput mRNA and miRNA profiling and isolated...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_03051048_v41_n6_p3699_Palm |
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todo:paper_03051048_v41_n6_p3699_Palm2023-10-03T15:21:32Z A systemic transcriptome analysis reveals the regulation of neural stem cell maintenance by an E2F1-miRNA feedback loop Palm, T. Hemmer, K. Winter, J. Fricke, I.B. Tarbashevich, K. Sadeghi Shakib, F. Rudolph, I.-M. Hillje, A.-L. De Luca, P. Bahnassawy, L. Madel, R. Viel, T. De Siervi, A. Jacobs, A.H. Diederichs, S. Schwamborn, J.C. messenger RNA microRNA microRNA 106a microRNA 17 microRNA 363 microRNA 92 transcription factor E2F1 unclassified drug animal cell article cell fate cell proliferation controlled study feedback system gene cluster gene expression profiling genetic transfection growth inhibition mouse nerve cell differentiation neural stem cell nonhuman priority journal protein analysis protein function protein RNA binding regulatory mechanism transcription termination transcriptomics upregulation Animals Cell Cycle Cells, Cultured E2F1 Transcription Factor Feedback, Physiological Gene Expression Profiling Gene Expression Regulation Mice MicroRNAs Neural Stem Cells Neurogenesis RNA, Messenger Mus Stem cell fate decisions are controlled by a molecular network in which transcription factors and miRNAs are of key importance. To systemically investigate their impact on neural stem cell (NSC) maintenance and neuronal commitment, we performed a high-throughput mRNA and miRNA profiling and isolated functional interaction networks of involved mechanisms. Thereby, we identified an E2F1-miRNA feedback loop as important regulator of NSC fate decisions. Although E2F1 supports NSC proliferation and represses transcription of miRNAs from the miR-17∼92 and miR-106a∼363 clusters, these miRNAs are transiently up-regulated at early stages of neuronal differentiation. In these early committed cells, increased miRNAs expression levels directly repress E2F1 mRNA levels and inhibit cellular proliferation. In mice, we demonstrated that these miRNAs are expressed in the neurogenic areas and that E2F1 inhibition represses NSC proliferation. The here presented data suggest a novel interaction mechanism between E2F1 and miR-17∼92 / miR-106a∼363 miRNAs in controlling NSC proliferation and neuronal differentiation. © The Author(s) 2013. Published by Oxford University Press. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03051048_v41_n6_p3699_Palm |
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Universidad de Buenos Aires |
| institution_str |
I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
messenger RNA microRNA microRNA 106a microRNA 17 microRNA 363 microRNA 92 transcription factor E2F1 unclassified drug animal cell article cell fate cell proliferation controlled study feedback system gene cluster gene expression profiling genetic transfection growth inhibition mouse nerve cell differentiation neural stem cell nonhuman priority journal protein analysis protein function protein RNA binding regulatory mechanism transcription termination transcriptomics upregulation Animals Cell Cycle Cells, Cultured E2F1 Transcription Factor Feedback, Physiological Gene Expression Profiling Gene Expression Regulation Mice MicroRNAs Neural Stem Cells Neurogenesis RNA, Messenger Mus |
| spellingShingle |
messenger RNA microRNA microRNA 106a microRNA 17 microRNA 363 microRNA 92 transcription factor E2F1 unclassified drug animal cell article cell fate cell proliferation controlled study feedback system gene cluster gene expression profiling genetic transfection growth inhibition mouse nerve cell differentiation neural stem cell nonhuman priority journal protein analysis protein function protein RNA binding regulatory mechanism transcription termination transcriptomics upregulation Animals Cell Cycle Cells, Cultured E2F1 Transcription Factor Feedback, Physiological Gene Expression Profiling Gene Expression Regulation Mice MicroRNAs Neural Stem Cells Neurogenesis RNA, Messenger Mus Palm, T. Hemmer, K. Winter, J. Fricke, I.B. Tarbashevich, K. Sadeghi Shakib, F. Rudolph, I.-M. Hillje, A.-L. De Luca, P. Bahnassawy, L. Madel, R. Viel, T. De Siervi, A. Jacobs, A.H. Diederichs, S. Schwamborn, J.C. A systemic transcriptome analysis reveals the regulation of neural stem cell maintenance by an E2F1-miRNA feedback loop |
| topic_facet |
messenger RNA microRNA microRNA 106a microRNA 17 microRNA 363 microRNA 92 transcription factor E2F1 unclassified drug animal cell article cell fate cell proliferation controlled study feedback system gene cluster gene expression profiling genetic transfection growth inhibition mouse nerve cell differentiation neural stem cell nonhuman priority journal protein analysis protein function protein RNA binding regulatory mechanism transcription termination transcriptomics upregulation Animals Cell Cycle Cells, Cultured E2F1 Transcription Factor Feedback, Physiological Gene Expression Profiling Gene Expression Regulation Mice MicroRNAs Neural Stem Cells Neurogenesis RNA, Messenger Mus |
| description |
Stem cell fate decisions are controlled by a molecular network in which transcription factors and miRNAs are of key importance. To systemically investigate their impact on neural stem cell (NSC) maintenance and neuronal commitment, we performed a high-throughput mRNA and miRNA profiling and isolated functional interaction networks of involved mechanisms. Thereby, we identified an E2F1-miRNA feedback loop as important regulator of NSC fate decisions. Although E2F1 supports NSC proliferation and represses transcription of miRNAs from the miR-17∼92 and miR-106a∼363 clusters, these miRNAs are transiently up-regulated at early stages of neuronal differentiation. In these early committed cells, increased miRNAs expression levels directly repress E2F1 mRNA levels and inhibit cellular proliferation. In mice, we demonstrated that these miRNAs are expressed in the neurogenic areas and that E2F1 inhibition represses NSC proliferation. The here presented data suggest a novel interaction mechanism between E2F1 and miR-17∼92 / miR-106a∼363 miRNAs in controlling NSC proliferation and neuronal differentiation. © The Author(s) 2013. Published by Oxford University Press. |
| format |
JOUR |
| author |
Palm, T. Hemmer, K. Winter, J. Fricke, I.B. Tarbashevich, K. Sadeghi Shakib, F. Rudolph, I.-M. Hillje, A.-L. De Luca, P. Bahnassawy, L. Madel, R. Viel, T. De Siervi, A. Jacobs, A.H. Diederichs, S. Schwamborn, J.C. |
| author_facet |
Palm, T. Hemmer, K. Winter, J. Fricke, I.B. Tarbashevich, K. Sadeghi Shakib, F. Rudolph, I.-M. Hillje, A.-L. De Luca, P. Bahnassawy, L. Madel, R. Viel, T. De Siervi, A. Jacobs, A.H. Diederichs, S. Schwamborn, J.C. |
| author_sort |
Palm, T. |
| title |
A systemic transcriptome analysis reveals the regulation of neural stem cell maintenance by an E2F1-miRNA feedback loop |
| title_short |
A systemic transcriptome analysis reveals the regulation of neural stem cell maintenance by an E2F1-miRNA feedback loop |
| title_full |
A systemic transcriptome analysis reveals the regulation of neural stem cell maintenance by an E2F1-miRNA feedback loop |
| title_fullStr |
A systemic transcriptome analysis reveals the regulation of neural stem cell maintenance by an E2F1-miRNA feedback loop |
| title_full_unstemmed |
A systemic transcriptome analysis reveals the regulation of neural stem cell maintenance by an E2F1-miRNA feedback loop |
| title_sort |
systemic transcriptome analysis reveals the regulation of neural stem cell maintenance by an e2f1-mirna feedback loop |
| url |
http://hdl.handle.net/20.500.12110/paper_03051048_v41_n6_p3699_Palm |
| work_keys_str_mv |
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