Progesterone receptor induces bcl-x expression through intragenic binding sites favoring RNA polymerase II elongation
Steroid receptors were classically described for regulating transcription by binding to target gene promoters. However, genome-wide studies reveal that steroid receptors-binding sites are mainly located at intragenic regions. To determine the role of these sites, we examined the effect of progestins...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_03051048_v41_n12_p6072_Bertucci |
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todo:paper_03051048_v41_n12_p6072_Bertucci2023-10-03T15:21:31Z Progesterone receptor induces bcl-x expression through intragenic binding sites favoring RNA polymerase II elongation Bertucci, P.Y. Nacht, A.S. Alló, M. Rocha-Viegas, L. Ballaré, C. Soronellas, D. Castellano, G. Zaurin, R. Kornblihtt, A.R. Beato, M. Vicent, G.P. Pecci, A. cyclic AMP responsive element binding protein binding protein histone acetyltransferase histone acetyltransferase GCN5 histone H3 histone H4 messenger RNA progesterone receptor protein bcl x protein SWI RNA polymerase II transcription factor SNF 3' untranslated region alternative RNA splicing article Bcl x gene binding site chromatin assembly and disassembly complex formation controlled study exon gene expression regulation gene function gene induction gene location gene targeting histone acetylation human human cell intron priority journal transcription elongation transcription initiation Alternative Splicing bcl-X Protein Binding Sites Cell Line, Tumor Chromatin CREB-Binding Protein Humans p300-CBP Transcription Factors Positive Transcriptional Elongation Factor B Promegestone Receptors, Progesterone RNA Polymerase II Transcription Elongation, Genetic Steroid receptors were classically described for regulating transcription by binding to target gene promoters. However, genome-wide studies reveal that steroid receptors-binding sites are mainly located at intragenic regions. To determine the role of these sites, we examined the effect of progestins on the transcription of the bcl-x gene, where only intragenic progesterone receptor-binding sites (PRbs) were identified. We found that in response to hormone treatment, the PR is recruited to these sites along with two histone acetyltransferases CREB-binding protein (CBP) and GCN5, leading to an increase in histone H3 and H4 acetylation and to the binding of the SWI/SNF complex. Concomitant, a more relaxed chromatin was detected along bcl-x gene mainly in the regions surrounding the intragenic PRbs. PR also mediated the recruitment of the positive elongation factor pTEFb, favoring RNA polymerase II (Pol II) elongation activity. Together these events promoted the re-distribution of the active Pol II toward the 3′-end of the gene and a decrease in the ratio between proximal and distal transcription. These results suggest a novel mechanism by which PR regulates gene expression by facilitating the proper passage of the polymerase along hormone-dependent genes. © 2013 The Author(s) 2013. Published by Oxford University Press. Fil:Bertucci, P.Y. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Alló, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rocha-Viegas, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ballaré, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Kornblihtt, A.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Vicent, G.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pecci, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03051048_v41_n12_p6072_Bertucci |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
cyclic AMP responsive element binding protein binding protein histone acetyltransferase histone acetyltransferase GCN5 histone H3 histone H4 messenger RNA progesterone receptor protein bcl x protein SWI RNA polymerase II transcription factor SNF 3' untranslated region alternative RNA splicing article Bcl x gene binding site chromatin assembly and disassembly complex formation controlled study exon gene expression regulation gene function gene induction gene location gene targeting histone acetylation human human cell intron priority journal transcription elongation transcription initiation Alternative Splicing bcl-X Protein Binding Sites Cell Line, Tumor Chromatin CREB-Binding Protein Humans p300-CBP Transcription Factors Positive Transcriptional Elongation Factor B Promegestone Receptors, Progesterone RNA Polymerase II Transcription Elongation, Genetic |
| spellingShingle |
cyclic AMP responsive element binding protein binding protein histone acetyltransferase histone acetyltransferase GCN5 histone H3 histone H4 messenger RNA progesterone receptor protein bcl x protein SWI RNA polymerase II transcription factor SNF 3' untranslated region alternative RNA splicing article Bcl x gene binding site chromatin assembly and disassembly complex formation controlled study exon gene expression regulation gene function gene induction gene location gene targeting histone acetylation human human cell intron priority journal transcription elongation transcription initiation Alternative Splicing bcl-X Protein Binding Sites Cell Line, Tumor Chromatin CREB-Binding Protein Humans p300-CBP Transcription Factors Positive Transcriptional Elongation Factor B Promegestone Receptors, Progesterone RNA Polymerase II Transcription Elongation, Genetic Bertucci, P.Y. Nacht, A.S. Alló, M. Rocha-Viegas, L. Ballaré, C. Soronellas, D. Castellano, G. Zaurin, R. Kornblihtt, A.R. Beato, M. Vicent, G.P. Pecci, A. Progesterone receptor induces bcl-x expression through intragenic binding sites favoring RNA polymerase II elongation |
| topic_facet |
cyclic AMP responsive element binding protein binding protein histone acetyltransferase histone acetyltransferase GCN5 histone H3 histone H4 messenger RNA progesterone receptor protein bcl x protein SWI RNA polymerase II transcription factor SNF 3' untranslated region alternative RNA splicing article Bcl x gene binding site chromatin assembly and disassembly complex formation controlled study exon gene expression regulation gene function gene induction gene location gene targeting histone acetylation human human cell intron priority journal transcription elongation transcription initiation Alternative Splicing bcl-X Protein Binding Sites Cell Line, Tumor Chromatin CREB-Binding Protein Humans p300-CBP Transcription Factors Positive Transcriptional Elongation Factor B Promegestone Receptors, Progesterone RNA Polymerase II Transcription Elongation, Genetic |
| description |
Steroid receptors were classically described for regulating transcription by binding to target gene promoters. However, genome-wide studies reveal that steroid receptors-binding sites are mainly located at intragenic regions. To determine the role of these sites, we examined the effect of progestins on the transcription of the bcl-x gene, where only intragenic progesterone receptor-binding sites (PRbs) were identified. We found that in response to hormone treatment, the PR is recruited to these sites along with two histone acetyltransferases CREB-binding protein (CBP) and GCN5, leading to an increase in histone H3 and H4 acetylation and to the binding of the SWI/SNF complex. Concomitant, a more relaxed chromatin was detected along bcl-x gene mainly in the regions surrounding the intragenic PRbs. PR also mediated the recruitment of the positive elongation factor pTEFb, favoring RNA polymerase II (Pol II) elongation activity. Together these events promoted the re-distribution of the active Pol II toward the 3′-end of the gene and a decrease in the ratio between proximal and distal transcription. These results suggest a novel mechanism by which PR regulates gene expression by facilitating the proper passage of the polymerase along hormone-dependent genes. © 2013 The Author(s) 2013. Published by Oxford University Press. |
| format |
JOUR |
| author |
Bertucci, P.Y. Nacht, A.S. Alló, M. Rocha-Viegas, L. Ballaré, C. Soronellas, D. Castellano, G. Zaurin, R. Kornblihtt, A.R. Beato, M. Vicent, G.P. Pecci, A. |
| author_facet |
Bertucci, P.Y. Nacht, A.S. Alló, M. Rocha-Viegas, L. Ballaré, C. Soronellas, D. Castellano, G. Zaurin, R. Kornblihtt, A.R. Beato, M. Vicent, G.P. Pecci, A. |
| author_sort |
Bertucci, P.Y. |
| title |
Progesterone receptor induces bcl-x expression through intragenic binding sites favoring RNA polymerase II elongation |
| title_short |
Progesterone receptor induces bcl-x expression through intragenic binding sites favoring RNA polymerase II elongation |
| title_full |
Progesterone receptor induces bcl-x expression through intragenic binding sites favoring RNA polymerase II elongation |
| title_fullStr |
Progesterone receptor induces bcl-x expression through intragenic binding sites favoring RNA polymerase II elongation |
| title_full_unstemmed |
Progesterone receptor induces bcl-x expression through intragenic binding sites favoring RNA polymerase II elongation |
| title_sort |
progesterone receptor induces bcl-x expression through intragenic binding sites favoring rna polymerase ii elongation |
| url |
http://hdl.handle.net/20.500.12110/paper_03051048_v41_n12_p6072_Bertucci |
| work_keys_str_mv |
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1807320653676675072 |