Heme synthesis in Trypanosoma cruzi: Influence of the strain and culture medium
1. 1. The activity of the following enzymes involved in the biosynthesis of porphyrins was determined in two strains of Trypanosoma cruzi (Y and CL) grown in two culture media (LIT and Warren): succinyl coenzyme A synthetase (Suc.CoA-S), 5-aminolevulinate synthetase (ALA-S), 4,5-dioxovaleric acid tr...
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todo:paper_03050491_v83_n1_p57_Salzman2023-10-03T15:21:18Z Heme synthesis in Trypanosoma cruzi: Influence of the strain and culture medium Salzman, T.A. Batlle, A.M.D.C. de Souza, W. 5 aminolevulinate synthase aminolevulinate aminotransferase aminotransferase ammonia lyase Ammonia Lyases ferrochelatase heme porphobilinogen synthase porphobilinogenase Succinate CoA Ligases succinyl coenzyme A synthetase animal article biosynthesis comparative study culture medium metabolism species difference Trypanosoma cruzi 5-Aminolevulinate Synthetase Ammonia-Lyases Animal Comparative Study Culture Media Ferrochelatase Heme Porphobilinogen Synthase Species Specificity Succinate-CoA Ligases Support, Non-U.S. Gov't Transaminases Trypanosoma cruzi 1. 1. The activity of the following enzymes involved in the biosynthesis of porphyrins was determined in two strains of Trypanosoma cruzi (Y and CL) grown in two culture media (LIT and Warren): succinyl coenzyme A synthetase (Suc.CoA-S), 5-aminolevulinate synthetase (ALA-S), 4,5-dioxovaleric acid transaminase (DOVA-T), 5-aminolevulinate dehydratase (ALA-D), porphobilinogenase (PBGase), deaminase and heme synthetase (Heme-S). The amount of 5-aminolevulinic acid (ALA) and porphobilinogen, porphyrins and heme was also determined. 2. 2. ALA and PGB were detected in both strains of T. cruzi. However, ALA was not detected in epimastigotes of the Y strain grown in the LIT medium. The content of ALA and PBG varied according to the strain and the growth medium. 3. 3. No free porphyrins and heme were detected in both strains of T. cruzi. 4. 4. The activity of Suc.CoA-S and DOVA-T was markedly influenced by the strains of the parasite and the growth medium. No significant DOVA-T activity was detected in epimastigotes of the CL strain grown in the Warren's medium. 5. 5. No significant activity of ALA-D, PBGase and deaminase was detected in T. cruzi. 6. 6. Activity of Heme-S was detected in both strains of T. cruzi when mesoporphyrin, protoporphyrin or deuteroporphyrin was used as substrate. The enzyme activity was influenced by the strain of the parasite, the growth medium and the substrate used. © 1986. Fil:Salzman, T.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Batlle, A.M.D.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03050491_v83_n1_p57_Salzman |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
5 aminolevulinate synthase aminolevulinate aminotransferase aminotransferase ammonia lyase Ammonia Lyases ferrochelatase heme porphobilinogen synthase porphobilinogenase Succinate CoA Ligases succinyl coenzyme A synthetase animal article biosynthesis comparative study culture medium metabolism species difference Trypanosoma cruzi 5-Aminolevulinate Synthetase Ammonia-Lyases Animal Comparative Study Culture Media Ferrochelatase Heme Porphobilinogen Synthase Species Specificity Succinate-CoA Ligases Support, Non-U.S. Gov't Transaminases Trypanosoma cruzi |
| spellingShingle |
5 aminolevulinate synthase aminolevulinate aminotransferase aminotransferase ammonia lyase Ammonia Lyases ferrochelatase heme porphobilinogen synthase porphobilinogenase Succinate CoA Ligases succinyl coenzyme A synthetase animal article biosynthesis comparative study culture medium metabolism species difference Trypanosoma cruzi 5-Aminolevulinate Synthetase Ammonia-Lyases Animal Comparative Study Culture Media Ferrochelatase Heme Porphobilinogen Synthase Species Specificity Succinate-CoA Ligases Support, Non-U.S. Gov't Transaminases Trypanosoma cruzi Salzman, T.A. Batlle, A.M.D.C. de Souza, W. Heme synthesis in Trypanosoma cruzi: Influence of the strain and culture medium |
| topic_facet |
5 aminolevulinate synthase aminolevulinate aminotransferase aminotransferase ammonia lyase Ammonia Lyases ferrochelatase heme porphobilinogen synthase porphobilinogenase Succinate CoA Ligases succinyl coenzyme A synthetase animal article biosynthesis comparative study culture medium metabolism species difference Trypanosoma cruzi 5-Aminolevulinate Synthetase Ammonia-Lyases Animal Comparative Study Culture Media Ferrochelatase Heme Porphobilinogen Synthase Species Specificity Succinate-CoA Ligases Support, Non-U.S. Gov't Transaminases Trypanosoma cruzi |
| description |
1. 1. The activity of the following enzymes involved in the biosynthesis of porphyrins was determined in two strains of Trypanosoma cruzi (Y and CL) grown in two culture media (LIT and Warren): succinyl coenzyme A synthetase (Suc.CoA-S), 5-aminolevulinate synthetase (ALA-S), 4,5-dioxovaleric acid transaminase (DOVA-T), 5-aminolevulinate dehydratase (ALA-D), porphobilinogenase (PBGase), deaminase and heme synthetase (Heme-S). The amount of 5-aminolevulinic acid (ALA) and porphobilinogen, porphyrins and heme was also determined. 2. 2. ALA and PGB were detected in both strains of T. cruzi. However, ALA was not detected in epimastigotes of the Y strain grown in the LIT medium. The content of ALA and PBG varied according to the strain and the growth medium. 3. 3. No free porphyrins and heme were detected in both strains of T. cruzi. 4. 4. The activity of Suc.CoA-S and DOVA-T was markedly influenced by the strains of the parasite and the growth medium. No significant DOVA-T activity was detected in epimastigotes of the CL strain grown in the Warren's medium. 5. 5. No significant activity of ALA-D, PBGase and deaminase was detected in T. cruzi. 6. 6. Activity of Heme-S was detected in both strains of T. cruzi when mesoporphyrin, protoporphyrin or deuteroporphyrin was used as substrate. The enzyme activity was influenced by the strain of the parasite, the growth medium and the substrate used. © 1986. |
| format |
JOUR |
| author |
Salzman, T.A. Batlle, A.M.D.C. de Souza, W. |
| author_facet |
Salzman, T.A. Batlle, A.M.D.C. de Souza, W. |
| author_sort |
Salzman, T.A. |
| title |
Heme synthesis in Trypanosoma cruzi: Influence of the strain and culture medium |
| title_short |
Heme synthesis in Trypanosoma cruzi: Influence of the strain and culture medium |
| title_full |
Heme synthesis in Trypanosoma cruzi: Influence of the strain and culture medium |
| title_fullStr |
Heme synthesis in Trypanosoma cruzi: Influence of the strain and culture medium |
| title_full_unstemmed |
Heme synthesis in Trypanosoma cruzi: Influence of the strain and culture medium |
| title_sort |
heme synthesis in trypanosoma cruzi: influence of the strain and culture medium |
| url |
http://hdl.handle.net/20.500.12110/paper_03050491_v83_n1_p57_Salzman |
| work_keys_str_mv |
AT salzmanta hemesynthesisintrypanosomacruziinfluenceofthestrainandculturemedium AT batlleamdc hemesynthesisintrypanosomacruziinfluenceofthestrainandculturemedium AT desouzaw hemesynthesisintrypanosomacruziinfluenceofthestrainandculturemedium |
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1807319516989882368 |