Purification and composition of glycoconjugates from Crithidia oncopelti

1. 1. Glycoconjugates were extracted with phenol-water from a symbiont containing strain of Crithidia oncopelti. 2. 2. A glycopeptide and two minor polysaccharides in a ratio of 30:5:1 were purified by subsequent DEAE-cellulose and Sephacryl S-300 column chromatographies. 3. 3. Mannose, galactose, g...

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Autores principales: Wolfenson, C., de Lederkremer, R.M.
Formato: JOUR
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_03050491_v77_n3_p555_Wolfenson
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
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spelling todo:paper_03050491_v77_n3_p555_Wolfenson2023-10-03T15:21:16Z Purification and composition of glycoconjugates from Crithidia oncopelti Wolfenson, C. de Lederkremer, R.M. 1. 1. Glycoconjugates were extracted with phenol-water from a symbiont containing strain of Crithidia oncopelti. 2. 2. A glycopeptide and two minor polysaccharides in a ratio of 30:5:1 were purified by subsequent DEAE-cellulose and Sephacryl S-300 column chromatographies. 3. 3. Mannose, galactose, glucose, fucose and arabinose in different proportions were detected in the three macromolecules by gas-liquid chromatography. Also about 1% phosphorus was determined. Mannose and galactose constitute more than 85% of the total sugar in the two most abundant fractions. 4. 4. Two glycoconjugates with lipidic and protein components were isolated from the aqueous and phenolic phase respectively. Galactose, mannose, arabinose and glucosamine were determined in the sugar moiety. Long chain bases and fatty acids have been identified in alkaline hydrolysates. © 1984. Fil:de Lederkremer, R.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03050491_v77_n3_p555_Wolfenson
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
description 1. 1. Glycoconjugates were extracted with phenol-water from a symbiont containing strain of Crithidia oncopelti. 2. 2. A glycopeptide and two minor polysaccharides in a ratio of 30:5:1 were purified by subsequent DEAE-cellulose and Sephacryl S-300 column chromatographies. 3. 3. Mannose, galactose, glucose, fucose and arabinose in different proportions were detected in the three macromolecules by gas-liquid chromatography. Also about 1% phosphorus was determined. Mannose and galactose constitute more than 85% of the total sugar in the two most abundant fractions. 4. 4. Two glycoconjugates with lipidic and protein components were isolated from the aqueous and phenolic phase respectively. Galactose, mannose, arabinose and glucosamine were determined in the sugar moiety. Long chain bases and fatty acids have been identified in alkaline hydrolysates. © 1984.
format JOUR
author Wolfenson, C.
de Lederkremer, R.M.
spellingShingle Wolfenson, C.
de Lederkremer, R.M.
Purification and composition of glycoconjugates from Crithidia oncopelti
author_facet Wolfenson, C.
de Lederkremer, R.M.
author_sort Wolfenson, C.
title Purification and composition of glycoconjugates from Crithidia oncopelti
title_short Purification and composition of glycoconjugates from Crithidia oncopelti
title_full Purification and composition of glycoconjugates from Crithidia oncopelti
title_fullStr Purification and composition of glycoconjugates from Crithidia oncopelti
title_full_unstemmed Purification and composition of glycoconjugates from Crithidia oncopelti
title_sort purification and composition of glycoconjugates from crithidia oncopelti
url http://hdl.handle.net/20.500.12110/paper_03050491_v77_n3_p555_Wolfenson
work_keys_str_mv AT wolfensonc purificationandcompositionofglycoconjugatesfromcrithidiaoncopelti
AT delederkremerrm purificationandcompositionofglycoconjugatesfromcrithidiaoncopelti
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