Influence of cellular functions on the evolution of persistent infections with Junin virus

Vero cell cultures persistently infected with Junin virus and subjected to different cultural conditions were established. The production of infectious plaque-forming virus, ts mutants and interfering viral particles was determined at different times during 110 days after infection. Carrier cultures...

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Detalles Bibliográficos
Autores principales: Candurra, N.A., Damonte, E.B.
Formato: JOUR
Materias:
cell culture
cell function
defective virus
heredity
in vitro study
junin virus
monkey
nonhuman
persistent infection
priority journal
temperature sensitive mutant
Animal
Arenaviridae
Arenaviruses, New World
Cells, Cultured
Cercopithecus aethiops
Cycloheximide
Dactinomycin
Mice
Proteins
Support, Non-U.S. Gov't
Temperature
Viral Interference
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_03048608_v86_n3-4_p275_Candurra
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_03048608_v86_n3-4_p275_Candurra
record_format dspace
spelling todo:paper_03048608_v86_n3-4_p275_Candurra2023-10-03T15:21:02Z Influence of cellular functions on the evolution of persistent infections with Junin virus Candurra, N.A. Damonte, E.B. cell culture cell function defective virus heredity in vitro study junin virus monkey nonhuman persistent infection priority journal temperature sensitive mutant Animal Arenaviridae Arenaviruses, New World Cells, Cultured Cercopithecus aethiops Cycloheximide Dactinomycin Mice Proteins Support, Non-U.S. Gov't Temperature Viral Interference Vero cell cultures persistently infected with Junin virus and subjected to different cultural conditions were established. The production of infectious plaque-forming virus, ts mutants and interfering viral particles was determined at different times during 110 days after infection. Carrier cultures maintained in stationary conditions continuously released PFU while proliferating persistent cultures exhibited a cyclical pattern which tends to a rapid PFU disappearance. Concomitantly, in stationary cultures the production of interfering particles was delayed and was lower than in actively growing persistent cells. The metabolic state of the infected cells did not affect the release of ts mutants. The results suggest that a cellular function is involved on the regulation of Junin virus persistent infections. © 1985 Springer-Verlag. Fil:Candurra, N.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Damonte, E.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03048608_v86_n3-4_p275_Candurra
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic cell culture
cell function
defective virus
heredity
in vitro study
junin virus
monkey
nonhuman
persistent infection
priority journal
temperature sensitive mutant
Animal
Arenaviridae
Arenaviruses, New World
Cells, Cultured
Cercopithecus aethiops
Cycloheximide
Dactinomycin
Mice
Proteins
Support, Non-U.S. Gov't
Temperature
Viral Interference
spellingShingle cell culture
cell function
defective virus
heredity
in vitro study
junin virus
monkey
nonhuman
persistent infection
priority journal
temperature sensitive mutant
Animal
Arenaviridae
Arenaviruses, New World
Cells, Cultured
Cercopithecus aethiops
Cycloheximide
Dactinomycin
Mice
Proteins
Support, Non-U.S. Gov't
Temperature
Viral Interference
Candurra, N.A.
Damonte, E.B.
Influence of cellular functions on the evolution of persistent infections with Junin virus
topic_facet cell culture
cell function
defective virus
heredity
in vitro study
junin virus
monkey
nonhuman
persistent infection
priority journal
temperature sensitive mutant
Animal
Arenaviridae
Arenaviruses, New World
Cells, Cultured
Cercopithecus aethiops
Cycloheximide
Dactinomycin
Mice
Proteins
Support, Non-U.S. Gov't
Temperature
Viral Interference
description Vero cell cultures persistently infected with Junin virus and subjected to different cultural conditions were established. The production of infectious plaque-forming virus, ts mutants and interfering viral particles was determined at different times during 110 days after infection. Carrier cultures maintained in stationary conditions continuously released PFU while proliferating persistent cultures exhibited a cyclical pattern which tends to a rapid PFU disappearance. Concomitantly, in stationary cultures the production of interfering particles was delayed and was lower than in actively growing persistent cells. The metabolic state of the infected cells did not affect the release of ts mutants. The results suggest that a cellular function is involved on the regulation of Junin virus persistent infections. © 1985 Springer-Verlag.
format JOUR
author Candurra, N.A.
Damonte, E.B.
author_facet Candurra, N.A.
Damonte, E.B.
author_sort Candurra, N.A.
title Influence of cellular functions on the evolution of persistent infections with Junin virus
title_short Influence of cellular functions on the evolution of persistent infections with Junin virus
title_full Influence of cellular functions on the evolution of persistent infections with Junin virus
title_fullStr Influence of cellular functions on the evolution of persistent infections with Junin virus
title_full_unstemmed Influence of cellular functions on the evolution of persistent infections with Junin virus
title_sort influence of cellular functions on the evolution of persistent infections with junin virus
url http://hdl.handle.net/20.500.12110/paper_03048608_v86_n3-4_p275_Candurra
work_keys_str_mv AT candurrana influenceofcellularfunctionsontheevolutionofpersistentinfectionswithjuninvirus
AT damonteeb influenceofcellularfunctionsontheevolutionofpersistentinfectionswithjuninvirus
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