First report of isolation and molecular characterization of bubaline herpesvirus 1 (BuHV1) from Argentinean water buffaloes

Herpesviruses have mainly co-evolved with their hosts for millions of years. However, bovine herpesvirus 1 (BoHV1) and related ruminant alphaherpesviruses have been reported to cross the species barrier. Bubaline herpesvirus 1 (BuHV1) is an alphaherpesvirus closely related to BoHV1 and BoHV5. Accord...

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Autores principales: Maidana, S.S., Konrad, J.L., Craig, M.I., Zabal, O., Mauroy, A., Thiry, E., Crudeli, G., Romera, S.A.
Formato: JOUR
Materias:
Bovine herpesvirus 1
Bubaline herpesvirus 1
Bubalus
Bubalus bubalis
Alphaherpesvirinae
animal
Argentina
buffalo
classification
genetics
herpes virus infection
isolation and purification
molecular genetics
phylogeny
veterinary
virology
Animals
Buffaloes
Herpesviridae Infections
Molecular Sequence Data
Phylogeny
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_03048608_v159_n11_p2917_Maidana
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
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spelling todo:paper_03048608_v159_n11_p2917_Maidana2023-10-03T15:21:01Z First report of isolation and molecular characterization of bubaline herpesvirus 1 (BuHV1) from Argentinean water buffaloes Maidana, S.S. Konrad, J.L. Craig, M.I. Zabal, O. Mauroy, A. Thiry, E. Crudeli, G. Romera, S.A. Bovine herpesvirus 1 Bubaline herpesvirus 1 Bubalus Bubalus bubalis Alphaherpesvirinae animal Argentina buffalo classification genetics herpes virus infection isolation and purification molecular genetics phylogeny veterinary virology Alphaherpesvirinae Animals Argentina Buffaloes Herpesviridae Infections Molecular Sequence Data Phylogeny Herpesviruses have mainly co-evolved with their hosts for millions of years. However, bovine herpesvirus 1 (BoHV1) and related ruminant alphaherpesviruses have been reported to cross the species barrier. Bubaline herpesvirus 1 (BuHV1) is an alphaherpesvirus closely related to BoHV1 and BoHV5. According to the serological cross-relationships between ruminant alphaherpesviruses, several surveys have studied the occurrence of BoHV1-related virus infection in wild and domestic ruminant species. Recent studies in Argentina showed an increase in serological prevalence against BoHV1 related viruses in water buffaloes (Bubalus bubalis) population. The aim of this study was to investigate the presence of related ruminant alphaherpesvirus in the Argentinean water buffalo population. BuHV1 was successfully isolated from 5 out of 225 buffaloes analyzed. One isolate was obtained from nasal secretions, and the others were from vaginal swabs. The buffaloes belonged to four different farms located in northeastern Argentina. The isolates were characterized as alphaherpesvirus by direct immunofluorescence using FITC-anti-BoHV1 IgG. Restriction analysis performed with BamHI and BstEII on the complete genome showed differences between the isolates and those from BoHV1 and BoHV5 subtypes. Phylogenetic analysis on both UL27 and US6 showed similarity in tree topology. While three of the isolates grouped together with sequences of BoHV5, two other isolates clustered separately. Genetic analysis of eight concatenated sequences from all isolates and references strains showed high nucleotide sequence identity between BuHV1 and BoHV5. While three of the isolates clustered together with the BoHV5 reference strain, the last two isolates were closely related to an Australian BuHV1 strain. To our knowledge, this is the first report on the isolation and molecular characterization of BuHV1 in South America. Phylogenetic analysis suggested that two different BuHV1 lineages circulate in the Argentinean water buffalo population. © 2014, Springer-Verlag Wien. Fil:Craig, M.I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Romera, S.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03048608_v159_n11_p2917_Maidana
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Bovine herpesvirus 1
Bubaline herpesvirus 1
Bubalus
Bubalus bubalis
Alphaherpesvirinae
animal
Argentina
buffalo
classification
genetics
herpes virus infection
isolation and purification
molecular genetics
phylogeny
veterinary
virology
Alphaherpesvirinae
Animals
Argentina
Buffaloes
Herpesviridae Infections
Molecular Sequence Data
Phylogeny
spellingShingle Bovine herpesvirus 1
Bubaline herpesvirus 1
Bubalus
Bubalus bubalis
Alphaherpesvirinae
animal
Argentina
buffalo
classification
genetics
herpes virus infection
isolation and purification
molecular genetics
phylogeny
veterinary
virology
Alphaherpesvirinae
Animals
Argentina
Buffaloes
Herpesviridae Infections
Molecular Sequence Data
Phylogeny
Maidana, S.S.
Konrad, J.L.
Craig, M.I.
Zabal, O.
Mauroy, A.
Thiry, E.
Crudeli, G.
Romera, S.A.
First report of isolation and molecular characterization of bubaline herpesvirus 1 (BuHV1) from Argentinean water buffaloes
topic_facet Bovine herpesvirus 1
Bubaline herpesvirus 1
Bubalus
Bubalus bubalis
Alphaherpesvirinae
animal
Argentina
buffalo
classification
genetics
herpes virus infection
isolation and purification
molecular genetics
phylogeny
veterinary
virology
Alphaherpesvirinae
Animals
Argentina
Buffaloes
Herpesviridae Infections
Molecular Sequence Data
Phylogeny
description Herpesviruses have mainly co-evolved with their hosts for millions of years. However, bovine herpesvirus 1 (BoHV1) and related ruminant alphaherpesviruses have been reported to cross the species barrier. Bubaline herpesvirus 1 (BuHV1) is an alphaherpesvirus closely related to BoHV1 and BoHV5. According to the serological cross-relationships between ruminant alphaherpesviruses, several surveys have studied the occurrence of BoHV1-related virus infection in wild and domestic ruminant species. Recent studies in Argentina showed an increase in serological prevalence against BoHV1 related viruses in water buffaloes (Bubalus bubalis) population. The aim of this study was to investigate the presence of related ruminant alphaherpesvirus in the Argentinean water buffalo population. BuHV1 was successfully isolated from 5 out of 225 buffaloes analyzed. One isolate was obtained from nasal secretions, and the others were from vaginal swabs. The buffaloes belonged to four different farms located in northeastern Argentina. The isolates were characterized as alphaherpesvirus by direct immunofluorescence using FITC-anti-BoHV1 IgG. Restriction analysis performed with BamHI and BstEII on the complete genome showed differences between the isolates and those from BoHV1 and BoHV5 subtypes. Phylogenetic analysis on both UL27 and US6 showed similarity in tree topology. While three of the isolates grouped together with sequences of BoHV5, two other isolates clustered separately. Genetic analysis of eight concatenated sequences from all isolates and references strains showed high nucleotide sequence identity between BuHV1 and BoHV5. While three of the isolates clustered together with the BoHV5 reference strain, the last two isolates were closely related to an Australian BuHV1 strain. To our knowledge, this is the first report on the isolation and molecular characterization of BuHV1 in South America. Phylogenetic analysis suggested that two different BuHV1 lineages circulate in the Argentinean water buffalo population. © 2014, Springer-Verlag Wien.
format JOUR
author Maidana, S.S.
Konrad, J.L.
Craig, M.I.
Zabal, O.
Mauroy, A.
Thiry, E.
Crudeli, G.
Romera, S.A.
author_facet Maidana, S.S.
Konrad, J.L.
Craig, M.I.
Zabal, O.
Mauroy, A.
Thiry, E.
Crudeli, G.
Romera, S.A.
author_sort Maidana, S.S.
title First report of isolation and molecular characterization of bubaline herpesvirus 1 (BuHV1) from Argentinean water buffaloes
title_short First report of isolation and molecular characterization of bubaline herpesvirus 1 (BuHV1) from Argentinean water buffaloes
title_full First report of isolation and molecular characterization of bubaline herpesvirus 1 (BuHV1) from Argentinean water buffaloes
title_fullStr First report of isolation and molecular characterization of bubaline herpesvirus 1 (BuHV1) from Argentinean water buffaloes
title_full_unstemmed First report of isolation and molecular characterization of bubaline herpesvirus 1 (BuHV1) from Argentinean water buffaloes
title_sort first report of isolation and molecular characterization of bubaline herpesvirus 1 (buhv1) from argentinean water buffaloes
url http://hdl.handle.net/20.500.12110/paper_03048608_v159_n11_p2917_Maidana
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