Synthesis and expression of viral antigens in Vero cells persistently infected with Junin virus

Two Vero cell lines persistently infected with XJCl3 and Cl67 strains of Junin virus and named V3 and V7, respectively, have been characterized with respect to the presence and expression of the nucleoprotein (N) and the glycoprotein precursor (GPC) viral genes. After the acute phase of infection, w...

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Autores principales:	Ellenberg, P., Edreira, M., Lozano, M., Scolaro, L.
Formato:	JOUR
Materias:	Animalia DNA viruses Junin virus nucleocapsid protein virus antigen virus envelope protein virus RNA animal article biosynthesis Cercopithecus genetics immunology Vero cell Animals Antigens, Viral Cercopithecus aethiops Nucleocapsid Proteins RNA, Viral Vero Cells Viral Envelope Proteins
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_03048608_v147_n8_p1543_Ellenberg
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_03048608_v147_n8_p1543_Ellenberg
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spelling	todo:paper_03048608_v147_n8_p1543_Ellenberg2023-10-03T15:20:58Z Synthesis and expression of viral antigens in Vero cells persistently infected with Junin virus Ellenberg, P. Edreira, M. Lozano, M. Scolaro, L. Animalia DNA viruses Junin virus nucleocapsid protein virus antigen virus envelope protein virus RNA animal article biosynthesis Cercopithecus genetics immunology Junin virus Vero cell Animals Antigens, Viral Cercopithecus aethiops Junin virus Nucleocapsid Proteins RNA, Viral Vero Cells Viral Envelope Proteins Two Vero cell lines persistently infected with XJCl3 and Cl67 strains of Junin virus and named V3 and V7, respectively, have been characterized with respect to the presence and expression of the nucleoprotein (N) and the glycoprotein precursor (GPC) viral genes. After the acute phase of infection, where a marked CPE and high titers of virus were obtained, JV persistently infected cells became morphologically undistinguishable from Vero cells and virus production dropped to undetectable levels. V3 and V7 were resistant to the superinfection with antigenically related viruses. This fact could not be attributed to the presence of defective interfering particles or non-infectious virus in the supernatant. Expression of N was consistently detected in both cultures and accumulation of two degradation products of N was evident during the late passages. Although no G1 (main surface glycoprotein) expression was observed, a marked fusogenic capacity was detected in both cultures indicating at least, the synthesis of a GPC derived fusogenic glycoprotein. Cell lysates from V3 and V7 subjected to RT-PCR, using specific primers for N gene, or to a nested RT-PCR using specific primers for GPC (G1 region) confirmed the presence of both viral genes. No viral DNA sequences could be detected in JV persistently infected cells. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03048608_v147_n8_p1543_Ellenberg
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Animalia DNA viruses Junin virus nucleocapsid protein virus antigen virus envelope protein virus RNA animal article biosynthesis Cercopithecus genetics immunology Junin virus Vero cell Animals Antigens, Viral Cercopithecus aethiops Junin virus Nucleocapsid Proteins RNA, Viral Vero Cells Viral Envelope Proteins
spellingShingle	Animalia DNA viruses Junin virus nucleocapsid protein virus antigen virus envelope protein virus RNA animal article biosynthesis Cercopithecus genetics immunology Junin virus Vero cell Animals Antigens, Viral Cercopithecus aethiops Junin virus Nucleocapsid Proteins RNA, Viral Vero Cells Viral Envelope Proteins Ellenberg, P. Edreira, M. Lozano, M. Scolaro, L. Synthesis and expression of viral antigens in Vero cells persistently infected with Junin virus
topic_facet	Animalia DNA viruses Junin virus nucleocapsid protein virus antigen virus envelope protein virus RNA animal article biosynthesis Cercopithecus genetics immunology Junin virus Vero cell Animals Antigens, Viral Cercopithecus aethiops Junin virus Nucleocapsid Proteins RNA, Viral Vero Cells Viral Envelope Proteins
description	Two Vero cell lines persistently infected with XJCl3 and Cl67 strains of Junin virus and named V3 and V7, respectively, have been characterized with respect to the presence and expression of the nucleoprotein (N) and the glycoprotein precursor (GPC) viral genes. After the acute phase of infection, where a marked CPE and high titers of virus were obtained, JV persistently infected cells became morphologically undistinguishable from Vero cells and virus production dropped to undetectable levels. V3 and V7 were resistant to the superinfection with antigenically related viruses. This fact could not be attributed to the presence of defective interfering particles or non-infectious virus in the supernatant. Expression of N was consistently detected in both cultures and accumulation of two degradation products of N was evident during the late passages. Although no G1 (main surface glycoprotein) expression was observed, a marked fusogenic capacity was detected in both cultures indicating at least, the synthesis of a GPC derived fusogenic glycoprotein. Cell lysates from V3 and V7 subjected to RT-PCR, using specific primers for N gene, or to a nested RT-PCR using specific primers for GPC (G1 region) confirmed the presence of both viral genes. No viral DNA sequences could be detected in JV persistently infected cells.
format	JOUR
author	Ellenberg, P. Edreira, M. Lozano, M. Scolaro, L.
author_facet	Ellenberg, P. Edreira, M. Lozano, M. Scolaro, L.
author_sort	Ellenberg, P.
title	Synthesis and expression of viral antigens in Vero cells persistently infected with Junin virus
title_short	Synthesis and expression of viral antigens in Vero cells persistently infected with Junin virus
title_full	Synthesis and expression of viral antigens in Vero cells persistently infected with Junin virus
title_fullStr	Synthesis and expression of viral antigens in Vero cells persistently infected with Junin virus
title_full_unstemmed	Synthesis and expression of viral antigens in Vero cells persistently infected with Junin virus
title_sort	synthesis and expression of viral antigens in vero cells persistently infected with junin virus
url	http://hdl.handle.net/20.500.12110/paper_03048608_v147_n8_p1543_Ellenberg
work_keys_str_mv	AT ellenbergp synthesisandexpressionofviralantigensinverocellspersistentlyinfectedwithjuninvirus AT edreiram synthesisandexpressionofviralantigensinverocellspersistentlyinfectedwithjuninvirus AT lozanom synthesisandexpressionofviralantigensinverocellspersistentlyinfectedwithjuninvirus AT scolarol synthesisandexpressionofviralantigensinverocellspersistentlyinfectedwithjuninvirus
_version_	1807321799726202880

Synthesis and expression of viral antigens in Vero cells persistently infected with Junin virus

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