Effects of reducing, oxidizing and alkylating agents on early steps of Junin virus multiplication

The action of reducing, oxidizing and thiol-alkylating agents on early steps of Junin virus (JV) multiplication in Vero cells was investigated. The presence of reducing agents during virus adsorption as well as incubation of viral particles with these compounds before infection enhanced JV infectivi...

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Autores principales: Raiger Iustman, L.J., Castilla, V., Meich, V., Mersich, S.E.
Formato: JOUR
Materias:
5,5' dithiobis(2 nitrobenzoic acid)
alkylating agent
concanavalin a
glycoprotein
junin virus
oxidizing agent
virus adsorption
virus infectivity
virus inhibition
virus replication
Alkylating Agents
Animals
Cercopithecus aethiops
Concanavalin A
Dithionitrobenzoic Acid
Dithiothreitol
Ficain
Junin virus
Membrane Glycoproteins
Mercaptoethanol
Oxidants
Periodic Acid
Phospholipase C
Potassium Compounds
Receptors, Virus
Reducing Agents
Sulfhydryl Reagents
Vero Cells
Virulence
Virus Replication
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_03048608_v143_n7_p1425_RaigerIustman
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_03048608_v143_n7_p1425_RaigerIustman
record_format dspace
spelling todo:paper_03048608_v143_n7_p1425_RaigerIustman2023-10-03T15:20:57Z Effects of reducing, oxidizing and alkylating agents on early steps of Junin virus multiplication Raiger Iustman, L.J. Castilla, V. Meich, V. Mersich, S.E. 5,5' dithiobis(2 nitrobenzoic acid) alkylating agent concanavalin a glycoprotein junin virus oxidizing agent virus adsorption virus infectivity virus inhibition virus replication Alkylating Agents Animals Cercopithecus aethiops Concanavalin A Dithionitrobenzoic Acid Dithiothreitol Ficain Junin virus Membrane Glycoproteins Mercaptoethanol Oxidants Periodic Acid Phospholipase C Potassium Compounds Receptors, Virus Reducing Agents Sulfhydryl Reagents Vero Cells Virulence Virus Replication Junin virus The action of reducing, oxidizing and thiol-alkylating agents on early steps of Junin virus (JV) multiplication in Vero cells was investigated. The presence of reducing agents during virus adsorption as well as incubation of viral particles with these compounds before infection enhanced JV infectivity. On the contrary, the thiol-alkylating agent 5, 5' dithiobis (2-nitrobenzoic acid) and the oxidizing compound potassium periodate showed an inhibitory effect, suggesting that sulfhydryl groups and certain sugar moieties of viral glycoproteins play an important role in the first steps of JV infection. Also enzymatic treatment of cell monolayers and addition of concanavalin A to cultures prior to infection suggest that cellular glycoproteins are involved in virus attachment. Fil:Raiger Iustman, L.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Castilla, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Mersich, S.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03048608_v143_n7_p1425_RaigerIustman
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic 5,5' dithiobis(2 nitrobenzoic acid)
alkylating agent
concanavalin a
glycoprotein
junin virus
oxidizing agent
virus adsorption
virus infectivity
virus inhibition
virus replication
Alkylating Agents
Animals
Cercopithecus aethiops
Concanavalin A
Dithionitrobenzoic Acid
Dithiothreitol
Ficain
Junin virus
Membrane Glycoproteins
Mercaptoethanol
Oxidants
Periodic Acid
Phospholipase C
Potassium Compounds
Receptors, Virus
Reducing Agents
Sulfhydryl Reagents
Vero Cells
Virulence
Virus Replication
Junin virus
spellingShingle 5,5' dithiobis(2 nitrobenzoic acid)
alkylating agent
concanavalin a
glycoprotein
junin virus
oxidizing agent
virus adsorption
virus infectivity
virus inhibition
virus replication
Alkylating Agents
Animals
Cercopithecus aethiops
Concanavalin A
Dithionitrobenzoic Acid
Dithiothreitol
Ficain
Junin virus
Membrane Glycoproteins
Mercaptoethanol
Oxidants
Periodic Acid
Phospholipase C
Potassium Compounds
Receptors, Virus
Reducing Agents
Sulfhydryl Reagents
Vero Cells
Virulence
Virus Replication
Junin virus
Raiger Iustman, L.J.
Castilla, V.
Meich, V.
Mersich, S.E.
Effects of reducing, oxidizing and alkylating agents on early steps of Junin virus multiplication
topic_facet 5,5' dithiobis(2 nitrobenzoic acid)
alkylating agent
concanavalin a
glycoprotein
junin virus
oxidizing agent
virus adsorption
virus infectivity
virus inhibition
virus replication
Alkylating Agents
Animals
Cercopithecus aethiops
Concanavalin A
Dithionitrobenzoic Acid
Dithiothreitol
Ficain
Junin virus
Membrane Glycoproteins
Mercaptoethanol
Oxidants
Periodic Acid
Phospholipase C
Potassium Compounds
Receptors, Virus
Reducing Agents
Sulfhydryl Reagents
Vero Cells
Virulence
Virus Replication
Junin virus
description The action of reducing, oxidizing and thiol-alkylating agents on early steps of Junin virus (JV) multiplication in Vero cells was investigated. The presence of reducing agents during virus adsorption as well as incubation of viral particles with these compounds before infection enhanced JV infectivity. On the contrary, the thiol-alkylating agent 5, 5' dithiobis (2-nitrobenzoic acid) and the oxidizing compound potassium periodate showed an inhibitory effect, suggesting that sulfhydryl groups and certain sugar moieties of viral glycoproteins play an important role in the first steps of JV infection. Also enzymatic treatment of cell monolayers and addition of concanavalin A to cultures prior to infection suggest that cellular glycoproteins are involved in virus attachment.
format JOUR
author Raiger Iustman, L.J.
Castilla, V.
Meich, V.
Mersich, S.E.
author_facet Raiger Iustman, L.J.
Castilla, V.
Meich, V.
Mersich, S.E.
author_sort Raiger Iustman, L.J.
title Effects of reducing, oxidizing and alkylating agents on early steps of Junin virus multiplication
title_short Effects of reducing, oxidizing and alkylating agents on early steps of Junin virus multiplication
title_full Effects of reducing, oxidizing and alkylating agents on early steps of Junin virus multiplication
title_fullStr Effects of reducing, oxidizing and alkylating agents on early steps of Junin virus multiplication
title_full_unstemmed Effects of reducing, oxidizing and alkylating agents on early steps of Junin virus multiplication
title_sort effects of reducing, oxidizing and alkylating agents on early steps of junin virus multiplication
url http://hdl.handle.net/20.500.12110/paper_03048608_v143_n7_p1425_RaigerIustman
work_keys_str_mv AT raigeriustmanlj effectsofreducingoxidizingandalkylatingagentsonearlystepsofjuninvirusmultiplication
AT castillav effectsofreducingoxidizingandalkylatingagentsonearlystepsofjuninvirusmultiplication
AT meichv effectsofreducingoxidizingandalkylatingagentsonearlystepsofjuninvirusmultiplication
AT mersichse effectsofreducingoxidizingandalkylatingagentsonearlystepsofjuninvirusmultiplication
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