The entry of Junin virus into Vero cells

The entry mechanism of Junin virus (JV) into Vero cells was studied analyzing the effect of lysosomotropic compounds and acid pH on JV infection. Ammonium chloride, amantadine, chlorpheniramine and procaine inhibited JV production. The action of ammonium chloride was exerted at early times of infect...

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Detalles Bibliográficos
Autores principales: Castilla, V., Mersich, S.E., Candurra, N.A., Damonte, E.B.
Formato: JOUR
Materias:
amantadine
ammonium chloride
chlorpheniramine
procaine
virus protein
animal
article
biosynthesis
drug effect
fluorescent antibody technique
Junin virus
membrane fusion
pH
physiology
Vero cell
virus replication
Amantadine
Ammonium Chloride
Animal
Chlorpheniramine
Fluorescent Antibody Technique
Hydrogen-Ion Concentration
Membrane Fusion
Procaine
Support, Non-U.S. Gov't
Vero Cells
Viral Proteins
Virus Replication
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_03048608_v136_n3-4_p363_Castilla
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:The entry mechanism of Junin virus (JV) into Vero cells was studied analyzing the effect of lysosomotropic compounds and acid pH on JV infection. Ammonium chloride, amantadine, chlorpheniramine and procaine inhibited JV production. The action of ammonium chloride was exerted at early times of infection. Virus internalization was inhibited and viral protein expression was not detected. When the extracellular medium was buffered at low pH, the ammonium chloride induced block on JV infection was overcome. Furthermore, JV was able to induce fusion of infected cells at pH 5.5 leading to polykaryoctye formation. Taken together, these results demonstrate that JV entry occurs through an endocytic mechanism requiring a low pH dependent membrane fusion. © 1994 Springer-Verlag.

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