The entry of Junin virus into Vero cells
The entry mechanism of Junin virus (JV) into Vero cells was studied analyzing the effect of lysosomotropic compounds and acid pH on JV infection. Ammonium chloride, amantadine, chlorpheniramine and procaine inhibited JV production. The action of ammonium chloride was exerted at early times of infect...
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todo:paper_03048608_v136_n3-4_p363_Castilla2023-10-03T15:20:55Z The entry of Junin virus into Vero cells Castilla, V. Mersich, S.E. Candurra, N.A. Damonte, E.B. amantadine ammonium chloride chlorpheniramine procaine virus protein animal article biosynthesis drug effect fluorescent antibody technique Junin virus membrane fusion pH physiology Vero cell virus replication Amantadine Ammonium Chloride Animal Chlorpheniramine Fluorescent Antibody Technique Hydrogen-Ion Concentration Junin virus Membrane Fusion Procaine Support, Non-U.S. Gov't Vero Cells Viral Proteins Virus Replication The entry mechanism of Junin virus (JV) into Vero cells was studied analyzing the effect of lysosomotropic compounds and acid pH on JV infection. Ammonium chloride, amantadine, chlorpheniramine and procaine inhibited JV production. The action of ammonium chloride was exerted at early times of infection. Virus internalization was inhibited and viral protein expression was not detected. When the extracellular medium was buffered at low pH, the ammonium chloride induced block on JV infection was overcome. Furthermore, JV was able to induce fusion of infected cells at pH 5.5 leading to polykaryoctye formation. Taken together, these results demonstrate that JV entry occurs through an endocytic mechanism requiring a low pH dependent membrane fusion. © 1994 Springer-Verlag. Fil:Castilla, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Mersich, S.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Candurra, N.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Damonte, E.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03048608_v136_n3-4_p363_Castilla |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
amantadine ammonium chloride chlorpheniramine procaine virus protein animal article biosynthesis drug effect fluorescent antibody technique Junin virus membrane fusion pH physiology Vero cell virus replication Amantadine Ammonium Chloride Animal Chlorpheniramine Fluorescent Antibody Technique Hydrogen-Ion Concentration Junin virus Membrane Fusion Procaine Support, Non-U.S. Gov't Vero Cells Viral Proteins Virus Replication |
| spellingShingle |
amantadine ammonium chloride chlorpheniramine procaine virus protein animal article biosynthesis drug effect fluorescent antibody technique Junin virus membrane fusion pH physiology Vero cell virus replication Amantadine Ammonium Chloride Animal Chlorpheniramine Fluorescent Antibody Technique Hydrogen-Ion Concentration Junin virus Membrane Fusion Procaine Support, Non-U.S. Gov't Vero Cells Viral Proteins Virus Replication Castilla, V. Mersich, S.E. Candurra, N.A. Damonte, E.B. The entry of Junin virus into Vero cells |
| topic_facet |
amantadine ammonium chloride chlorpheniramine procaine virus protein animal article biosynthesis drug effect fluorescent antibody technique Junin virus membrane fusion pH physiology Vero cell virus replication Amantadine Ammonium Chloride Animal Chlorpheniramine Fluorescent Antibody Technique Hydrogen-Ion Concentration Junin virus Membrane Fusion Procaine Support, Non-U.S. Gov't Vero Cells Viral Proteins Virus Replication |
| description |
The entry mechanism of Junin virus (JV) into Vero cells was studied analyzing the effect of lysosomotropic compounds and acid pH on JV infection. Ammonium chloride, amantadine, chlorpheniramine and procaine inhibited JV production. The action of ammonium chloride was exerted at early times of infection. Virus internalization was inhibited and viral protein expression was not detected. When the extracellular medium was buffered at low pH, the ammonium chloride induced block on JV infection was overcome. Furthermore, JV was able to induce fusion of infected cells at pH 5.5 leading to polykaryoctye formation. Taken together, these results demonstrate that JV entry occurs through an endocytic mechanism requiring a low pH dependent membrane fusion. © 1994 Springer-Verlag. |
| format |
JOUR |
| author |
Castilla, V. Mersich, S.E. Candurra, N.A. Damonte, E.B. |
| author_facet |
Castilla, V. Mersich, S.E. Candurra, N.A. Damonte, E.B. |
| author_sort |
Castilla, V. |
| title |
The entry of Junin virus into Vero cells |
| title_short |
The entry of Junin virus into Vero cells |
| title_full |
The entry of Junin virus into Vero cells |
| title_fullStr |
The entry of Junin virus into Vero cells |
| title_full_unstemmed |
The entry of Junin virus into Vero cells |
| title_sort |
entry of junin virus into vero cells |
| url |
http://hdl.handle.net/20.500.12110/paper_03048608_v136_n3-4_p363_Castilla |
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