Effect of glucose on induction of δ-aminolevulinic acid synthase, ferrochelatase and cytochrome P-450 hemoproteins in isolated rat hepatocytes by phenobarbital and lead
In the present work we have been able to demonstrate the phenobarbital and lead exert an inducing effect on the biosynthesis of δ-aminovulenic acid synthase, ferrochelatase and cytochrome P-450 hemoproteins in isolated rat hepatocytes of normal adult rats. Dibutyryl cyclic AMP enhances the induction...
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todo:paper_03044165_v841_n2_p186_Canepa2023-10-03T15:20:51Z Effect of glucose on induction of δ-aminolevulinic acid synthase, ferrochelatase and cytochrome P-450 hemoproteins in isolated rat hepatocytes by phenobarbital and lead Canepa, E.T. Llambias, E.B.C. Grinstein, M. (Rat hepatocyte) Cytochrome P-450 Enzyme induction Ferrochelatase Glucose δ-Aminolevulinate synthase 5 aminolevulinate synthase adrenalin bucladesine cycloheximide cytochrome p450 ferrochelatase glucose insulin lead phenobarbital dose response dose time effect relation drug response in vitro study liver liver cell nonhuman priority journal 5-Aminolevulinate Synthetase Animal Bucladesine Cells, Cultured Cycloheximide Cytochrome P-450 Enzyme System Enzyme Induction Epinephrine Ferrochelatase Glucose Hemeproteins Insulin Lead Liver Lyases Male Phenobarbital Rats Subcellular Fractions Support, Non-U.S. Gov't Theophylline In the present work we have been able to demonstrate the phenobarbital and lead exert an inducing effect on the biosynthesis of δ-aminovulenic acid synthase, ferrochelatase and cytochrome P-450 hemoproteins in isolated rat hepatocytes of normal adult rats. Dibutyryl cyclic AMP enhances the induction effect produced by phenobarbital in this in vitro system. Glucose inhibits the induction of δ-aminolevulinic acid synthase and ferrochelatase. This repression effect can be reversed with increasing concentrations of dibutyryl cyclic AMP. No glucose effect was observed on the phenobarbital- and lead-mediated inductions of cytochrome P-450. The present results add more experimental evidence to support the concept that the last enzyme of the heme pathway is inducible, and as such may have a significant role in regulatory mechanisms of porphyrin and heme biosynthesis. © 1985. Fil:Canepa, E.T. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Llambias, E.B.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Grinstein, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03044165_v841_n2_p186_Canepa |
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Universidad de Buenos Aires |
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I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
(Rat hepatocyte) Cytochrome P-450 Enzyme induction Ferrochelatase Glucose δ-Aminolevulinate synthase 5 aminolevulinate synthase adrenalin bucladesine cycloheximide cytochrome p450 ferrochelatase glucose insulin lead phenobarbital dose response dose time effect relation drug response in vitro study liver liver cell nonhuman priority journal 5-Aminolevulinate Synthetase Animal Bucladesine Cells, Cultured Cycloheximide Cytochrome P-450 Enzyme System Enzyme Induction Epinephrine Ferrochelatase Glucose Hemeproteins Insulin Lead Liver Lyases Male Phenobarbital Rats Subcellular Fractions Support, Non-U.S. Gov't Theophylline |
| spellingShingle |
(Rat hepatocyte) Cytochrome P-450 Enzyme induction Ferrochelatase Glucose δ-Aminolevulinate synthase 5 aminolevulinate synthase adrenalin bucladesine cycloheximide cytochrome p450 ferrochelatase glucose insulin lead phenobarbital dose response dose time effect relation drug response in vitro study liver liver cell nonhuman priority journal 5-Aminolevulinate Synthetase Animal Bucladesine Cells, Cultured Cycloheximide Cytochrome P-450 Enzyme System Enzyme Induction Epinephrine Ferrochelatase Glucose Hemeproteins Insulin Lead Liver Lyases Male Phenobarbital Rats Subcellular Fractions Support, Non-U.S. Gov't Theophylline Canepa, E.T. Llambias, E.B.C. Grinstein, M. Effect of glucose on induction of δ-aminolevulinic acid synthase, ferrochelatase and cytochrome P-450 hemoproteins in isolated rat hepatocytes by phenobarbital and lead |
| topic_facet |
(Rat hepatocyte) Cytochrome P-450 Enzyme induction Ferrochelatase Glucose δ-Aminolevulinate synthase 5 aminolevulinate synthase adrenalin bucladesine cycloheximide cytochrome p450 ferrochelatase glucose insulin lead phenobarbital dose response dose time effect relation drug response in vitro study liver liver cell nonhuman priority journal 5-Aminolevulinate Synthetase Animal Bucladesine Cells, Cultured Cycloheximide Cytochrome P-450 Enzyme System Enzyme Induction Epinephrine Ferrochelatase Glucose Hemeproteins Insulin Lead Liver Lyases Male Phenobarbital Rats Subcellular Fractions Support, Non-U.S. Gov't Theophylline |
| description |
In the present work we have been able to demonstrate the phenobarbital and lead exert an inducing effect on the biosynthesis of δ-aminovulenic acid synthase, ferrochelatase and cytochrome P-450 hemoproteins in isolated rat hepatocytes of normal adult rats. Dibutyryl cyclic AMP enhances the induction effect produced by phenobarbital in this in vitro system. Glucose inhibits the induction of δ-aminolevulinic acid synthase and ferrochelatase. This repression effect can be reversed with increasing concentrations of dibutyryl cyclic AMP. No glucose effect was observed on the phenobarbital- and lead-mediated inductions of cytochrome P-450. The present results add more experimental evidence to support the concept that the last enzyme of the heme pathway is inducible, and as such may have a significant role in regulatory mechanisms of porphyrin and heme biosynthesis. © 1985. |
| format |
JOUR |
| author |
Canepa, E.T. Llambias, E.B.C. Grinstein, M. |
| author_facet |
Canepa, E.T. Llambias, E.B.C. Grinstein, M. |
| author_sort |
Canepa, E.T. |
| title |
Effect of glucose on induction of δ-aminolevulinic acid synthase, ferrochelatase and cytochrome P-450 hemoproteins in isolated rat hepatocytes by phenobarbital and lead |
| title_short |
Effect of glucose on induction of δ-aminolevulinic acid synthase, ferrochelatase and cytochrome P-450 hemoproteins in isolated rat hepatocytes by phenobarbital and lead |
| title_full |
Effect of glucose on induction of δ-aminolevulinic acid synthase, ferrochelatase and cytochrome P-450 hemoproteins in isolated rat hepatocytes by phenobarbital and lead |
| title_fullStr |
Effect of glucose on induction of δ-aminolevulinic acid synthase, ferrochelatase and cytochrome P-450 hemoproteins in isolated rat hepatocytes by phenobarbital and lead |
| title_full_unstemmed |
Effect of glucose on induction of δ-aminolevulinic acid synthase, ferrochelatase and cytochrome P-450 hemoproteins in isolated rat hepatocytes by phenobarbital and lead |
| title_sort |
effect of glucose on induction of δ-aminolevulinic acid synthase, ferrochelatase and cytochrome p-450 hemoproteins in isolated rat hepatocytes by phenobarbital and lead |
| url |
http://hdl.handle.net/20.500.12110/paper_03044165_v841_n2_p186_Canepa |
| work_keys_str_mv |
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| _version_ |
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