Lipid carriers in the synthesis of high-mannose glycoproteins in algae
Particulate preparations from the chlorophyta Prototheca zopfii catalyze the incorporation of mannose and N-acetylglucosamine into glycolipids. These had been characterized as lipid monophosphate mannose, lipid pyrophosphate N,N′-diacetylchitobiose and various lipid-linked oligosaccharides containin...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_03044165_v586_n3_p545_Romero |
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todo:paper_03044165_v586_n3_p545_Romero2023-10-03T15:20:50Z Lipid carriers in the synthesis of high-mannose glycoproteins in algae Romero, P.A. Hopp, H.E. Pont Lezica, R. (Alga) Bacitracin Dolichyl phosphate Glycoprotein synthesis High-mannose glycoprotein bacitracin glycolipid glycoprotein mannose n acetylglucosamine oligosaccharide alga article biosynthesis intracellular membrane metabolism Acetylglucosamine Algae, Green Bacitracin Glycolipids Glycoproteins Intracellular Membranes Mannose Oligosaccharides Particulate preparations from the chlorophyta Prototheca zopfii catalyze the incorporation of mannose and N-acetylglucosamine into glycolipids. These had been characterized as lipid monophosphate mannose, lipid pyrophosphate N,N′-diacetylchitobiose and various lipid-linked oligosaccharides containing two N-acetylglucosamine residues plus a variable number of mannose residues. The lipid moiety has the properties expected for dolichyl phosphate. The oligosaccharide-linked lipids serve as precursors for the formation of a polymer sensible to pronase digestion. The oligosaccharide is linked by N-glycosidic linkage to an asparagine residue. In longer incubation periods, a polymer insensitive to pronase hydrolysis, but precipitable by copper salts such as cell wall mannans is formed. Polymer formation is inhibited by 1 mM bacitracin. The reactions leading to the formation of the mannoprotein were found associated to the rough endoplasmic reticulum. The synthesis of mannans was found to occur in the Golgi vesicles. © 1979. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03044165_v586_n3_p545_Romero |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
(Alga) Bacitracin Dolichyl phosphate Glycoprotein synthesis High-mannose glycoprotein bacitracin glycolipid glycoprotein mannose n acetylglucosamine oligosaccharide alga article biosynthesis intracellular membrane metabolism Acetylglucosamine Algae, Green Bacitracin Glycolipids Glycoproteins Intracellular Membranes Mannose Oligosaccharides |
| spellingShingle |
(Alga) Bacitracin Dolichyl phosphate Glycoprotein synthesis High-mannose glycoprotein bacitracin glycolipid glycoprotein mannose n acetylglucosamine oligosaccharide alga article biosynthesis intracellular membrane metabolism Acetylglucosamine Algae, Green Bacitracin Glycolipids Glycoproteins Intracellular Membranes Mannose Oligosaccharides Romero, P.A. Hopp, H.E. Pont Lezica, R. Lipid carriers in the synthesis of high-mannose glycoproteins in algae |
| topic_facet |
(Alga) Bacitracin Dolichyl phosphate Glycoprotein synthesis High-mannose glycoprotein bacitracin glycolipid glycoprotein mannose n acetylglucosamine oligosaccharide alga article biosynthesis intracellular membrane metabolism Acetylglucosamine Algae, Green Bacitracin Glycolipids Glycoproteins Intracellular Membranes Mannose Oligosaccharides |
| description |
Particulate preparations from the chlorophyta Prototheca zopfii catalyze the incorporation of mannose and N-acetylglucosamine into glycolipids. These had been characterized as lipid monophosphate mannose, lipid pyrophosphate N,N′-diacetylchitobiose and various lipid-linked oligosaccharides containing two N-acetylglucosamine residues plus a variable number of mannose residues. The lipid moiety has the properties expected for dolichyl phosphate. The oligosaccharide-linked lipids serve as precursors for the formation of a polymer sensible to pronase digestion. The oligosaccharide is linked by N-glycosidic linkage to an asparagine residue. In longer incubation periods, a polymer insensitive to pronase hydrolysis, but precipitable by copper salts such as cell wall mannans is formed. Polymer formation is inhibited by 1 mM bacitracin. The reactions leading to the formation of the mannoprotein were found associated to the rough endoplasmic reticulum. The synthesis of mannans was found to occur in the Golgi vesicles. © 1979. |
| format |
JOUR |
| author |
Romero, P.A. Hopp, H.E. Pont Lezica, R. |
| author_facet |
Romero, P.A. Hopp, H.E. Pont Lezica, R. |
| author_sort |
Romero, P.A. |
| title |
Lipid carriers in the synthesis of high-mannose glycoproteins in algae |
| title_short |
Lipid carriers in the synthesis of high-mannose glycoproteins in algae |
| title_full |
Lipid carriers in the synthesis of high-mannose glycoproteins in algae |
| title_fullStr |
Lipid carriers in the synthesis of high-mannose glycoproteins in algae |
| title_full_unstemmed |
Lipid carriers in the synthesis of high-mannose glycoproteins in algae |
| title_sort |
lipid carriers in the synthesis of high-mannose glycoproteins in algae |
| url |
http://hdl.handle.net/20.500.12110/paper_03044165_v586_n3_p545_Romero |
| work_keys_str_mv |
AT romeropa lipidcarriersinthesynthesisofhighmannoseglycoproteinsinalgae AT hopphe lipidcarriersinthesynthesisofhighmannoseglycoproteinsinalgae AT pontlezicar lipidcarriersinthesynthesisofhighmannoseglycoproteinsinalgae |
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1807317274158170112 |