Glycogen synthesis in the fungus Neurospora crassa

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An enzymic activity, obtained from Neurospora crassa, catalyzing the incorporation of [14C]glucose from ADP-[14C]glucose into a glucan of the glycogen type, is described. The properties of the ADPglucose: glycogen glucosyltransferase as compared with those of the already known UDP glucose: glycogen...

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Detalles Bibliográficos
Autores principales: Fontana, J.D., Krisman, C.R.
Formato: JOUR
Materias:
glycogen
radioisotope
adenosine diphosphate glucose c 14
animal experiment
fungus
glucose c 14
in vitro study
neurospora crassa
Adenosine Diphosphate Glucose
Comparative Study
Enzyme Activation
Glycogen Synthase
Neurospora
Neurospora crassa
Uridine Diphosphate Glucose
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_03044165_v540_n2_p183_Fontana
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:An enzymic activity, obtained from Neurospora crassa, catalyzing the incorporation of [14C]glucose from ADP-[14C]glucose into a glucan of the glycogen type, is described. The properties of the ADPglucose: glycogen glucosyltransferase as compared with those of the already known UDP glucose: glycogen glucosyltransferase were studied. The radioactive products obtained with UDP-[14C]glucose or ADP-[14C]glucose released all the radioactivity as maltose after α or β amylase treatment. Glucose 6-phosphate stimulated the synthetase when UDP-[14C]glucose was the substrate but the stimulation was much greater with ADP-[14C]glucose as glucosyl donor. Glucose 6-phosphate plus EGTA gave maximal stimulation. The system was completely dependent on the presence of a 'primer' of the α 1 → 4 glucan type. © 1978.

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