Glycogen synthesis in the fungus Neurospora crassa
An enzymic activity, obtained from Neurospora crassa, catalyzing the incorporation of [14C]glucose from ADP-[14C]glucose into a glucan of the glycogen type, is described. The properties of the ADPglucose: glycogen glucosyltransferase as compared with those of the already known UDP glucose: glycogen...
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| Formato: | JOUR |
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_03044165_v540_n2_p183_Fontana |
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todo:paper_03044165_v540_n2_p183_Fontana2023-10-03T15:20:49Z Glycogen synthesis in the fungus Neurospora crassa Fontana, J.D. Krisman, C.R. glycogen radioisotope adenosine diphosphate glucose c 14 animal experiment fungus glucose c 14 in vitro study neurospora crassa Adenosine Diphosphate Glucose Comparative Study Enzyme Activation Glycogen Synthase Neurospora Neurospora crassa Uridine Diphosphate Glucose An enzymic activity, obtained from Neurospora crassa, catalyzing the incorporation of [14C]glucose from ADP-[14C]glucose into a glucan of the glycogen type, is described. The properties of the ADPglucose: glycogen glucosyltransferase as compared with those of the already known UDP glucose: glycogen glucosyltransferase were studied. The radioactive products obtained with UDP-[14C]glucose or ADP-[14C]glucose released all the radioactivity as maltose after α or β amylase treatment. Glucose 6-phosphate stimulated the synthetase when UDP-[14C]glucose was the substrate but the stimulation was much greater with ADP-[14C]glucose as glucosyl donor. Glucose 6-phosphate plus EGTA gave maximal stimulation. The system was completely dependent on the presence of a 'primer' of the α 1 → 4 glucan type. © 1978. Fil:Fontana, J.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03044165_v540_n2_p183_Fontana |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
glycogen radioisotope adenosine diphosphate glucose c 14 animal experiment fungus glucose c 14 in vitro study neurospora crassa Adenosine Diphosphate Glucose Comparative Study Enzyme Activation Glycogen Synthase Neurospora Neurospora crassa Uridine Diphosphate Glucose |
| spellingShingle |
glycogen radioisotope adenosine diphosphate glucose c 14 animal experiment fungus glucose c 14 in vitro study neurospora crassa Adenosine Diphosphate Glucose Comparative Study Enzyme Activation Glycogen Synthase Neurospora Neurospora crassa Uridine Diphosphate Glucose Fontana, J.D. Krisman, C.R. Glycogen synthesis in the fungus Neurospora crassa |
| topic_facet |
glycogen radioisotope adenosine diphosphate glucose c 14 animal experiment fungus glucose c 14 in vitro study neurospora crassa Adenosine Diphosphate Glucose Comparative Study Enzyme Activation Glycogen Synthase Neurospora Neurospora crassa Uridine Diphosphate Glucose |
| description |
An enzymic activity, obtained from Neurospora crassa, catalyzing the incorporation of [14C]glucose from ADP-[14C]glucose into a glucan of the glycogen type, is described. The properties of the ADPglucose: glycogen glucosyltransferase as compared with those of the already known UDP glucose: glycogen glucosyltransferase were studied. The radioactive products obtained with UDP-[14C]glucose or ADP-[14C]glucose released all the radioactivity as maltose after α or β amylase treatment. Glucose 6-phosphate stimulated the synthetase when UDP-[14C]glucose was the substrate but the stimulation was much greater with ADP-[14C]glucose as glucosyl donor. Glucose 6-phosphate plus EGTA gave maximal stimulation. The system was completely dependent on the presence of a 'primer' of the α 1 → 4 glucan type. © 1978. |
| format |
JOUR |
| author |
Fontana, J.D. Krisman, C.R. |
| author_facet |
Fontana, J.D. Krisman, C.R. |
| author_sort |
Fontana, J.D. |
| title |
Glycogen synthesis in the fungus Neurospora crassa |
| title_short |
Glycogen synthesis in the fungus Neurospora crassa |
| title_full |
Glycogen synthesis in the fungus Neurospora crassa |
| title_fullStr |
Glycogen synthesis in the fungus Neurospora crassa |
| title_full_unstemmed |
Glycogen synthesis in the fungus Neurospora crassa |
| title_sort |
glycogen synthesis in the fungus neurospora crassa |
| url |
http://hdl.handle.net/20.500.12110/paper_03044165_v540_n2_p183_Fontana |
| work_keys_str_mv |
AT fontanajd glycogensynthesisinthefungusneurosporacrassa AT krismancr glycogensynthesisinthefungusneurosporacrassa |
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1782028142635909120 |