Deoxycorticosterone stimulates the activity of nicotinamide adenine dinucleotide phosphate-diaphorase/nitric oxide synthase immunoreactivity in hypothalamic nuclei of rats

Mineralocorticoids (MC) play an important role in development of salt appetite. Part of this effect involves the hypothalamic paraventricular (PVN) and supraoptic (SON) nuclei, in which MC treatment increases arginine vasopressin (AVP) synthesis and release. Since the AVP system is also modulated by...

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Detalles Bibliográficos
Autores principales: Saravia, F.E., Pietranera, L., Lima, A., Roig, P., Revsin, Y., De Nicola, A.F.
Formato: JOUR
Materias:
Mineralocorticoids
Nicotinamide dinucleotide diphoshate-diaphorase
Nitric oxide synthase
Salt appetite
argipressin
cytochrome b5 reductase
deoxycorticosterone acetate
messenger RNA
nicotinamide adenine dinucleotide phosphate
nitric oxide
nitric oxide synthase
sodium chloride
animal cell
animal experiment
animal tissue
anterior hypothalamus
appetite
brain nerve cell
cell count
controlled study
drug effect
enzyme activation
enzyme activity
food intake
hypothalamus nucleus
immunocompetent cell
immunoreactivity
male
nonhuman
priority journal
rat
review
supraoptic nucleus
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_03043940_v329_n3_p344_Saravia
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:Mineralocorticoids (MC) play an important role in development of salt appetite. Part of this effect involves the hypothalamic paraventricular (PVN) and supraoptic (SON) nuclei, in which MC treatment increases arginine vasopressin (AVP) synthesis and release. Since the AVP system is also modulated by nitric oxide (NO), we studied if deoxycorticosterone acetate (DOCA) treatment changed the number of nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) active neurons and neuronal NO synthase (nNOS)-immunoreactive (IR) cells in the PVN and SON. After four injections of DOCA (10 mg/rat per day), rats developed a salt appetite and increased NADPH-d active and nNOS-IR neurons in both nuclei. A single DOCA injection did not change salt consumption or nNOS-IR cells, but increased the number of NADPH-d positive neurons in the PVN only. Therefore, while acute MC treatment stimulated the activity of pre-existing enzyme, chronic steroid treatment recruited additional neurons showing nNOS immunoreactivity/NADPH-d activity. These data suggest a role for NO produced in the PVN and SON in DOCA stimulatory effects on AVP mRNA and salt appetite. © 2002 Published by Elsevier Science Ireland Ltd.

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