Deoxycorticosterone stimulates the activity of nicotinamide adenine dinucleotide phosphate-diaphorase/nitric oxide synthase immunoreactivity in hypothalamic nuclei of rats

Mineralocorticoids (MC) play an important role in development of salt appetite. Part of this effect involves the hypothalamic paraventricular (PVN) and supraoptic (SON) nuclei, in which MC treatment increases arginine vasopressin (AVP) synthesis and release. Since the AVP system is also modulated by...

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Autores principales: Saravia, F.E., Pietranera, L., Lima, A., Roig, P., Revsin, Y., De Nicola, A.F.
Formato: JOUR
Materias:
Mineralocorticoids
Nicotinamide dinucleotide diphoshate-diaphorase
Nitric oxide synthase
Salt appetite
argipressin
cytochrome b5 reductase
deoxycorticosterone acetate
messenger RNA
nicotinamide adenine dinucleotide phosphate
nitric oxide
nitric oxide synthase
sodium chloride
animal cell
animal experiment
animal tissue
anterior hypothalamus
appetite
brain nerve cell
cell count
controlled study
drug effect
enzyme activation
enzyme activity
food intake
hypothalamus nucleus
immunocompetent cell
immunoreactivity
male
nonhuman
priority journal
rat
review
supraoptic nucleus
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_03043940_v329_n3_p344_Saravia
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
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spelling todo:paper_03043940_v329_n3_p344_Saravia2023-10-03T15:20:18Z Deoxycorticosterone stimulates the activity of nicotinamide adenine dinucleotide phosphate-diaphorase/nitric oxide synthase immunoreactivity in hypothalamic nuclei of rats Saravia, F.E. Pietranera, L. Lima, A. Roig, P. Revsin, Y. De Nicola, A.F. Mineralocorticoids Nicotinamide dinucleotide diphoshate-diaphorase Nitric oxide synthase Salt appetite argipressin cytochrome b5 reductase deoxycorticosterone acetate messenger RNA nicotinamide adenine dinucleotide phosphate nitric oxide nitric oxide synthase sodium chloride animal cell animal experiment animal tissue anterior hypothalamus appetite brain nerve cell cell count controlled study drug effect enzyme activation enzyme activity food intake hypothalamus nucleus immunocompetent cell immunoreactivity male nonhuman priority journal rat review supraoptic nucleus Mineralocorticoids (MC) play an important role in development of salt appetite. Part of this effect involves the hypothalamic paraventricular (PVN) and supraoptic (SON) nuclei, in which MC treatment increases arginine vasopressin (AVP) synthesis and release. Since the AVP system is also modulated by nitric oxide (NO), we studied if deoxycorticosterone acetate (DOCA) treatment changed the number of nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) active neurons and neuronal NO synthase (nNOS)-immunoreactive (IR) cells in the PVN and SON. After four injections of DOCA (10 mg/rat per day), rats developed a salt appetite and increased NADPH-d active and nNOS-IR neurons in both nuclei. A single DOCA injection did not change salt consumption or nNOS-IR cells, but increased the number of NADPH-d positive neurons in the PVN only. Therefore, while acute MC treatment stimulated the activity of pre-existing enzyme, chronic steroid treatment recruited additional neurons showing nNOS immunoreactivity/NADPH-d activity. These data suggest a role for NO produced in the PVN and SON in DOCA stimulatory effects on AVP mRNA and salt appetite. © 2002 Published by Elsevier Science Ireland Ltd. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03043940_v329_n3_p344_Saravia
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Mineralocorticoids
Nicotinamide dinucleotide diphoshate-diaphorase
Nitric oxide synthase
Salt appetite
argipressin
cytochrome b5 reductase
deoxycorticosterone acetate
messenger RNA
nicotinamide adenine dinucleotide phosphate
nitric oxide
nitric oxide synthase
sodium chloride
animal cell
animal experiment
animal tissue
anterior hypothalamus
appetite
brain nerve cell
cell count
controlled study
drug effect
enzyme activation
enzyme activity
food intake
hypothalamus nucleus
immunocompetent cell
immunoreactivity
male
nonhuman
priority journal
rat
review
supraoptic nucleus
spellingShingle Mineralocorticoids
Nicotinamide dinucleotide diphoshate-diaphorase
Nitric oxide synthase
Salt appetite
argipressin
cytochrome b5 reductase
deoxycorticosterone acetate
messenger RNA
nicotinamide adenine dinucleotide phosphate
nitric oxide
nitric oxide synthase
sodium chloride
animal cell
animal experiment
animal tissue
anterior hypothalamus
appetite
brain nerve cell
cell count
controlled study
drug effect
enzyme activation
enzyme activity
food intake
hypothalamus nucleus
immunocompetent cell
immunoreactivity
male
nonhuman
priority journal
rat
review
supraoptic nucleus
Saravia, F.E.
Pietranera, L.
Lima, A.
Roig, P.
Revsin, Y.
De Nicola, A.F.
Deoxycorticosterone stimulates the activity of nicotinamide adenine dinucleotide phosphate-diaphorase/nitric oxide synthase immunoreactivity in hypothalamic nuclei of rats
topic_facet Mineralocorticoids
Nicotinamide dinucleotide diphoshate-diaphorase
Nitric oxide synthase
Salt appetite
argipressin
cytochrome b5 reductase
deoxycorticosterone acetate
messenger RNA
nicotinamide adenine dinucleotide phosphate
nitric oxide
nitric oxide synthase
sodium chloride
animal cell
animal experiment
animal tissue
anterior hypothalamus
appetite
brain nerve cell
cell count
controlled study
drug effect
enzyme activation
enzyme activity
food intake
hypothalamus nucleus
immunocompetent cell
immunoreactivity
male
nonhuman
priority journal
rat
review
supraoptic nucleus
description Mineralocorticoids (MC) play an important role in development of salt appetite. Part of this effect involves the hypothalamic paraventricular (PVN) and supraoptic (SON) nuclei, in which MC treatment increases arginine vasopressin (AVP) synthesis and release. Since the AVP system is also modulated by nitric oxide (NO), we studied if deoxycorticosterone acetate (DOCA) treatment changed the number of nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) active neurons and neuronal NO synthase (nNOS)-immunoreactive (IR) cells in the PVN and SON. After four injections of DOCA (10 mg/rat per day), rats developed a salt appetite and increased NADPH-d active and nNOS-IR neurons in both nuclei. A single DOCA injection did not change salt consumption or nNOS-IR cells, but increased the number of NADPH-d positive neurons in the PVN only. Therefore, while acute MC treatment stimulated the activity of pre-existing enzyme, chronic steroid treatment recruited additional neurons showing nNOS immunoreactivity/NADPH-d activity. These data suggest a role for NO produced in the PVN and SON in DOCA stimulatory effects on AVP mRNA and salt appetite. © 2002 Published by Elsevier Science Ireland Ltd.
format JOUR
author Saravia, F.E.
Pietranera, L.
Lima, A.
Roig, P.
Revsin, Y.
De Nicola, A.F.
author_facet Saravia, F.E.
Pietranera, L.
Lima, A.
Roig, P.
Revsin, Y.
De Nicola, A.F.
author_sort Saravia, F.E.
title Deoxycorticosterone stimulates the activity of nicotinamide adenine dinucleotide phosphate-diaphorase/nitric oxide synthase immunoreactivity in hypothalamic nuclei of rats
title_short Deoxycorticosterone stimulates the activity of nicotinamide adenine dinucleotide phosphate-diaphorase/nitric oxide synthase immunoreactivity in hypothalamic nuclei of rats
title_full Deoxycorticosterone stimulates the activity of nicotinamide adenine dinucleotide phosphate-diaphorase/nitric oxide synthase immunoreactivity in hypothalamic nuclei of rats
title_fullStr Deoxycorticosterone stimulates the activity of nicotinamide adenine dinucleotide phosphate-diaphorase/nitric oxide synthase immunoreactivity in hypothalamic nuclei of rats
title_full_unstemmed Deoxycorticosterone stimulates the activity of nicotinamide adenine dinucleotide phosphate-diaphorase/nitric oxide synthase immunoreactivity in hypothalamic nuclei of rats
title_sort deoxycorticosterone stimulates the activity of nicotinamide adenine dinucleotide phosphate-diaphorase/nitric oxide synthase immunoreactivity in hypothalamic nuclei of rats
url http://hdl.handle.net/20.500.12110/paper_03043940_v329_n3_p344_Saravia
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AT pietraneral deoxycorticosteronestimulatestheactivityofnicotinamideadeninedinucleotidephosphatediaphorasenitricoxidesynthaseimmunoreactivityinhypothalamicnucleiofrats
AT limaa deoxycorticosteronestimulatestheactivityofnicotinamideadeninedinucleotidephosphatediaphorasenitricoxidesynthaseimmunoreactivityinhypothalamicnucleiofrats
AT roigp deoxycorticosteronestimulatestheactivityofnicotinamideadeninedinucleotidephosphatediaphorasenitricoxidesynthaseimmunoreactivityinhypothalamicnucleiofrats
AT revsiny deoxycorticosteronestimulatestheactivityofnicotinamideadeninedinucleotidephosphatediaphorasenitricoxidesynthaseimmunoreactivityinhypothalamicnucleiofrats
AT denicolaaf deoxycorticosteronestimulatestheactivityofnicotinamideadeninedinucleotidephosphatediaphorasenitricoxidesynthaseimmunoreactivityinhypothalamicnucleiofrats
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