Roscovitine inhibits ongoing DNA synthesis in human cervical cancer

The effect of roscovitine, a purine analogue and cyclin dependent kinase inhibitor, on DNA synthesis rate in tissue mini-units obtained from human cervical cancers was investigated. Roscovitine (100 μM) gave a DNA synthesis rate inhibition by 61% (P<0.0001; range 23-93%) within 30 min of incubati...

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Detalles Bibliográficos
Autores principales: Vitali, L., Yakisich, J.Sebastian, Vita, M.F., Fernandez, A., Settembrini, L., Siden, A., Cruz, M., Carminatti, H., Casas, O., Idoyaga Vargas, V.
Formato: JOUR
Materias:
Cervical cancer
Cyclin dependent kinases
DNA synthesis
Roscovitine
roscovitine
adult
aged
article
cancer chemotherapy
cancer staging
cell proliferation
clinical article
concentration response
controlled study
DNA synthesis inhibition
drug effect
female
human
human tissue
priority journal
uterine cervix cancer
Adult
Aged
Antineoplastic Agents
Cyclin-Dependent Kinases
DNA
Dose-Response Relationship, Drug
Enzyme Inhibitors
Female
Humans
Middle Aged
Purines
Time Factors
Uterine Cervical Neoplasms
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_03043835_v180_n1_p7_Vitali
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:The effect of roscovitine, a purine analogue and cyclin dependent kinase inhibitor, on DNA synthesis rate in tissue mini-units obtained from human cervical cancers was investigated. Roscovitine (100 μM) gave a DNA synthesis rate inhibition by 61% (P<0.0001; range 23-93%) within 30 min of incubation. This inhibitory effect was concentration-dependent. The results suggest that the inhibition of tumor DNA synthesis rate is due to a direct effect on the DNA synthesis machinery via presently unknown mechanisms. In addition, the potential application of CDKs inhibitors as preventive agents is discussed. © 2002 Elsevier Science Ireland Ltd. All rights reserved.

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