Roscovitine inhibits ongoing DNA synthesis in human cervical cancer

The effect of roscovitine, a purine analogue and cyclin dependent kinase inhibitor, on DNA synthesis rate in tissue mini-units obtained from human cervical cancers was investigated. Roscovitine (100 μM) gave a DNA synthesis rate inhibition by 61% (P<0.0001; range 23-93%) within 30 min of incubati...

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Autores principales:	Vitali, L., Yakisich, J.Sebastian, Vita, M.F., Fernandez, A., Settembrini, L., Siden, A., Cruz, M., Carminatti, H., Casas, O., Idoyaga Vargas, V.
Formato:	JOUR
Materias:	Cervical cancer Cyclin dependent kinases DNA synthesis Roscovitine roscovitine adult aged article cancer chemotherapy cancer staging cell proliferation clinical article concentration response controlled study DNA synthesis inhibition drug effect female human human tissue priority journal uterine cervix cancer Adult Aged Antineoplastic Agents Cyclin-Dependent Kinases DNA Dose-Response Relationship, Drug Enzyme Inhibitors Female Humans Middle Aged Purines Time Factors Uterine Cervical Neoplasms
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_03043835_v180_n1_p7_Vitali
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_03043835_v180_n1_p7_Vitali2023-10-03T15:20:04Z Roscovitine inhibits ongoing DNA synthesis in human cervical cancer Vitali, L. Yakisich, J.Sebastian Vita, M.F. Fernandez, A. Settembrini, L. Siden, A. Cruz, M. Carminatti, H. Casas, O. Idoyaga Vargas, V. Cervical cancer Cyclin dependent kinases DNA synthesis Roscovitine roscovitine adult aged article cancer chemotherapy cancer staging cell proliferation clinical article concentration response controlled study DNA synthesis DNA synthesis inhibition drug effect female human human tissue priority journal uterine cervix cancer Adult Aged Antineoplastic Agents Cyclin-Dependent Kinases DNA Dose-Response Relationship, Drug Enzyme Inhibitors Female Humans Middle Aged Purines Time Factors Uterine Cervical Neoplasms The effect of roscovitine, a purine analogue and cyclin dependent kinase inhibitor, on DNA synthesis rate in tissue mini-units obtained from human cervical cancers was investigated. Roscovitine (100 μM) gave a DNA synthesis rate inhibition by 61% (P<0.0001; range 23-93%) within 30 min of incubation. This inhibitory effect was concentration-dependent. The results suggest that the inhibition of tumor DNA synthesis rate is due to a direct effect on the DNA synthesis machinery via presently unknown mechanisms. In addition, the potential application of CDKs inhibitors as preventive agents is discussed. © 2002 Elsevier Science Ireland Ltd. All rights reserved. Fil:Vita, M.F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Carminatti, H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03043835_v180_n1_p7_Vitali
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Cervical cancer Cyclin dependent kinases DNA synthesis Roscovitine roscovitine adult aged article cancer chemotherapy cancer staging cell proliferation clinical article concentration response controlled study DNA synthesis DNA synthesis inhibition drug effect female human human tissue priority journal uterine cervix cancer Adult Aged Antineoplastic Agents Cyclin-Dependent Kinases DNA Dose-Response Relationship, Drug Enzyme Inhibitors Female Humans Middle Aged Purines Time Factors Uterine Cervical Neoplasms
spellingShingle	Cervical cancer Cyclin dependent kinases DNA synthesis Roscovitine roscovitine adult aged article cancer chemotherapy cancer staging cell proliferation clinical article concentration response controlled study DNA synthesis DNA synthesis inhibition drug effect female human human tissue priority journal uterine cervix cancer Adult Aged Antineoplastic Agents Cyclin-Dependent Kinases DNA Dose-Response Relationship, Drug Enzyme Inhibitors Female Humans Middle Aged Purines Time Factors Uterine Cervical Neoplasms Vitali, L. Yakisich, J.Sebastian Vita, M.F. Fernandez, A. Settembrini, L. Siden, A. Cruz, M. Carminatti, H. Casas, O. Idoyaga Vargas, V. Roscovitine inhibits ongoing DNA synthesis in human cervical cancer
topic_facet	Cervical cancer Cyclin dependent kinases DNA synthesis Roscovitine roscovitine adult aged article cancer chemotherapy cancer staging cell proliferation clinical article concentration response controlled study DNA synthesis DNA synthesis inhibition drug effect female human human tissue priority journal uterine cervix cancer Adult Aged Antineoplastic Agents Cyclin-Dependent Kinases DNA Dose-Response Relationship, Drug Enzyme Inhibitors Female Humans Middle Aged Purines Time Factors Uterine Cervical Neoplasms
description	The effect of roscovitine, a purine analogue and cyclin dependent kinase inhibitor, on DNA synthesis rate in tissue mini-units obtained from human cervical cancers was investigated. Roscovitine (100 μM) gave a DNA synthesis rate inhibition by 61% (P<0.0001; range 23-93%) within 30 min of incubation. This inhibitory effect was concentration-dependent. The results suggest that the inhibition of tumor DNA synthesis rate is due to a direct effect on the DNA synthesis machinery via presently unknown mechanisms. In addition, the potential application of CDKs inhibitors as preventive agents is discussed. © 2002 Elsevier Science Ireland Ltd. All rights reserved.
format	JOUR
author	Vitali, L. Yakisich, J.Sebastian Vita, M.F. Fernandez, A. Settembrini, L. Siden, A. Cruz, M. Carminatti, H. Casas, O. Idoyaga Vargas, V.
author_facet	Vitali, L. Yakisich, J.Sebastian Vita, M.F. Fernandez, A. Settembrini, L. Siden, A. Cruz, M. Carminatti, H. Casas, O. Idoyaga Vargas, V.
author_sort	Vitali, L.
title	Roscovitine inhibits ongoing DNA synthesis in human cervical cancer
title_short	Roscovitine inhibits ongoing DNA synthesis in human cervical cancer
title_full	Roscovitine inhibits ongoing DNA synthesis in human cervical cancer
title_fullStr	Roscovitine inhibits ongoing DNA synthesis in human cervical cancer
title_full_unstemmed	Roscovitine inhibits ongoing DNA synthesis in human cervical cancer
title_sort	roscovitine inhibits ongoing dna synthesis in human cervical cancer
url	http://hdl.handle.net/20.500.12110/paper_03043835_v180_n1_p7_Vitali
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_version_	1807323947768741888

Roscovitine inhibits ongoing DNA synthesis in human cervical cancer

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