VIP induces the decidualization program and conditions the immunoregulation of the implantation process
The decidualization process involves phenotype and functional changes on endometrial cells and the modulation of mediators with immunoregulatory properties as the vasoactive intestinal peptide (VIP). We investigate VIP contribution to the decidualization program and to immunoregulation throughout th...
Guardado en:
| Autores principales: | , , , , , , , , , , , |
|---|---|
| Formato: | JOUR |
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_03037207_v460_n_p63_Grasso |
| Aporte de: |
| id |
todo:paper_03037207_v460_n_p63_Grasso |
|---|---|
| record_format |
dspace |
| spelling |
todo:paper_03037207_v460_n_p63_Grasso2023-10-03T15:19:59Z VIP induces the decidualization program and conditions the immunoregulation of the implantation process Grasso, E. Gori, S. Paparini, D. Soczewski, E. Fernández, L. Gallino, L. Salamone, G. Martinez, G. Irigoyen, M. Ruhlmann, C. Pérez Leirós, C. Ramhorst, R. Decidualization Immunoregulation Implantation VIP cysteine rich protein 61 dibutyrylcytidine 3',5' phosphate interleukin 8 medroxyprogesterone membrane protein monocyte chemotactic protein 1 prolactin protein KLF13 stromal cell derived factor 1 unclassified drug vasoactive intestinal polypeptide vasoactive intestinal polypeptide receptor biological marker vasoactive intestinal polypeptide Article blastocyst CD4+ T lymphocyte controlled study decidualization dendritic cell human human cell immunoregulation in vitro study nidation priority journal protein expression regulatory T lymphocyte stromal cell line tumor spheroid biological model cell line conditioned medium cytology decidua drug effect endometrium female immunological tolerance immunology metabolism nidation pharmacology stroma cell trophoblast tumor microenvironment Biomarkers Blastocyst Cell Line Cellular Microenvironment Culture Media, Conditioned Decidua Embryo Implantation Endometrium Female Humans Immune Tolerance Models, Biological Stromal Cells Trophoblasts Vasoactive Intestinal Peptide The decidualization process involves phenotype and functional changes on endometrial cells and the modulation of mediators with immunoregulatory properties as the vasoactive intestinal peptide (VIP). We investigate VIP contribution to the decidualization program and to immunoregulation throughout the human embryo implantation process. The decidualization of Human endometrial stromal cell line (HESC) with Medroxyprogesterone-dibutyryl-cAMP increased VIP/VPAC-receptors system. In fact, VIP could induce decidualization increasing differentiation markers (IGFBP1, PRL, KLF13/KLF9 ratio, CXCL12, CXCL8 and CCL2) and allowing Blastocyst-like spheroids (BLS) invasion in an in vitro model of embryo implantation. Focus on the tolerogenic effects, decidualized cells induced a semi-mature profile on maternal dendritic cells; restrained CD4+ cells recruitment while increased regulatory T-cells recruitment. Interestingly, the human blastocyst conditioned media from developmentally impaired embryos diminished the invasion and T-regulatory cells recruitment in these settings. These evidences suggest that VIP contributes to the implantation process inducing decidualization, allowing BLS invasion and favoring a tolerogenic micro-environment. © 2017 Elsevier B.V. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03037207_v460_n_p63_Grasso |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Decidualization Immunoregulation Implantation VIP cysteine rich protein 61 dibutyrylcytidine 3',5' phosphate interleukin 8 medroxyprogesterone membrane protein monocyte chemotactic protein 1 prolactin protein KLF13 stromal cell derived factor 1 unclassified drug vasoactive intestinal polypeptide vasoactive intestinal polypeptide receptor biological marker vasoactive intestinal polypeptide Article blastocyst CD4+ T lymphocyte controlled study decidualization dendritic cell human human cell immunoregulation in vitro study nidation priority journal protein expression regulatory T lymphocyte stromal cell line tumor spheroid biological model cell line conditioned medium cytology decidua drug effect endometrium female immunological tolerance immunology metabolism nidation pharmacology stroma cell trophoblast tumor microenvironment Biomarkers Blastocyst Cell Line Cellular Microenvironment Culture Media, Conditioned Decidua Embryo Implantation Endometrium Female Humans Immune Tolerance Models, Biological Stromal Cells Trophoblasts Vasoactive Intestinal Peptide |
| spellingShingle |
Decidualization Immunoregulation Implantation VIP cysteine rich protein 61 dibutyrylcytidine 3',5' phosphate interleukin 8 medroxyprogesterone membrane protein monocyte chemotactic protein 1 prolactin protein KLF13 stromal cell derived factor 1 unclassified drug vasoactive intestinal polypeptide vasoactive intestinal polypeptide receptor biological marker vasoactive intestinal polypeptide Article blastocyst CD4+ T lymphocyte controlled study decidualization dendritic cell human human cell immunoregulation in vitro study nidation priority journal protein expression regulatory T lymphocyte stromal cell line tumor spheroid biological model cell line conditioned medium cytology decidua drug effect endometrium female immunological tolerance immunology metabolism nidation pharmacology stroma cell trophoblast tumor microenvironment Biomarkers Blastocyst Cell Line Cellular Microenvironment Culture Media, Conditioned Decidua Embryo Implantation Endometrium Female Humans Immune Tolerance Models, Biological Stromal Cells Trophoblasts Vasoactive Intestinal Peptide Grasso, E. Gori, S. Paparini, D. Soczewski, E. Fernández, L. Gallino, L. Salamone, G. Martinez, G. Irigoyen, M. Ruhlmann, C. Pérez Leirós, C. Ramhorst, R. VIP induces the decidualization program and conditions the immunoregulation of the implantation process |
| topic_facet |
Decidualization Immunoregulation Implantation VIP cysteine rich protein 61 dibutyrylcytidine 3',5' phosphate interleukin 8 medroxyprogesterone membrane protein monocyte chemotactic protein 1 prolactin protein KLF13 stromal cell derived factor 1 unclassified drug vasoactive intestinal polypeptide vasoactive intestinal polypeptide receptor biological marker vasoactive intestinal polypeptide Article blastocyst CD4+ T lymphocyte controlled study decidualization dendritic cell human human cell immunoregulation in vitro study nidation priority journal protein expression regulatory T lymphocyte stromal cell line tumor spheroid biological model cell line conditioned medium cytology decidua drug effect endometrium female immunological tolerance immunology metabolism nidation pharmacology stroma cell trophoblast tumor microenvironment Biomarkers Blastocyst Cell Line Cellular Microenvironment Culture Media, Conditioned Decidua Embryo Implantation Endometrium Female Humans Immune Tolerance Models, Biological Stromal Cells Trophoblasts Vasoactive Intestinal Peptide |
| description |
The decidualization process involves phenotype and functional changes on endometrial cells and the modulation of mediators with immunoregulatory properties as the vasoactive intestinal peptide (VIP). We investigate VIP contribution to the decidualization program and to immunoregulation throughout the human embryo implantation process. The decidualization of Human endometrial stromal cell line (HESC) with Medroxyprogesterone-dibutyryl-cAMP increased VIP/VPAC-receptors system. In fact, VIP could induce decidualization increasing differentiation markers (IGFBP1, PRL, KLF13/KLF9 ratio, CXCL12, CXCL8 and CCL2) and allowing Blastocyst-like spheroids (BLS) invasion in an in vitro model of embryo implantation. Focus on the tolerogenic effects, decidualized cells induced a semi-mature profile on maternal dendritic cells; restrained CD4+ cells recruitment while increased regulatory T-cells recruitment. Interestingly, the human blastocyst conditioned media from developmentally impaired embryos diminished the invasion and T-regulatory cells recruitment in these settings. These evidences suggest that VIP contributes to the implantation process inducing decidualization, allowing BLS invasion and favoring a tolerogenic micro-environment. © 2017 Elsevier B.V. |
| format |
JOUR |
| author |
Grasso, E. Gori, S. Paparini, D. Soczewski, E. Fernández, L. Gallino, L. Salamone, G. Martinez, G. Irigoyen, M. Ruhlmann, C. Pérez Leirós, C. Ramhorst, R. |
| author_facet |
Grasso, E. Gori, S. Paparini, D. Soczewski, E. Fernández, L. Gallino, L. Salamone, G. Martinez, G. Irigoyen, M. Ruhlmann, C. Pérez Leirós, C. Ramhorst, R. |
| author_sort |
Grasso, E. |
| title |
VIP induces the decidualization program and conditions the immunoregulation of the implantation process |
| title_short |
VIP induces the decidualization program and conditions the immunoregulation of the implantation process |
| title_full |
VIP induces the decidualization program and conditions the immunoregulation of the implantation process |
| title_fullStr |
VIP induces the decidualization program and conditions the immunoregulation of the implantation process |
| title_full_unstemmed |
VIP induces the decidualization program and conditions the immunoregulation of the implantation process |
| title_sort |
vip induces the decidualization program and conditions the immunoregulation of the implantation process |
| url |
http://hdl.handle.net/20.500.12110/paper_03037207_v460_n_p63_Grasso |
| work_keys_str_mv |
AT grassoe vipinducesthedecidualizationprogramandconditionstheimmunoregulationoftheimplantationprocess AT goris vipinducesthedecidualizationprogramandconditionstheimmunoregulationoftheimplantationprocess AT paparinid vipinducesthedecidualizationprogramandconditionstheimmunoregulationoftheimplantationprocess AT soczewskie vipinducesthedecidualizationprogramandconditionstheimmunoregulationoftheimplantationprocess AT fernandezl vipinducesthedecidualizationprogramandconditionstheimmunoregulationoftheimplantationprocess AT gallinol vipinducesthedecidualizationprogramandconditionstheimmunoregulationoftheimplantationprocess AT salamoneg vipinducesthedecidualizationprogramandconditionstheimmunoregulationoftheimplantationprocess AT martinezg vipinducesthedecidualizationprogramandconditionstheimmunoregulationoftheimplantationprocess AT irigoyenm vipinducesthedecidualizationprogramandconditionstheimmunoregulationoftheimplantationprocess AT ruhlmannc vipinducesthedecidualizationprogramandconditionstheimmunoregulationoftheimplantationprocess AT perezleirosc vipinducesthedecidualizationprogramandconditionstheimmunoregulationoftheimplantationprocess AT ramhorstr vipinducesthedecidualizationprogramandconditionstheimmunoregulationoftheimplantationprocess |
| _version_ |
1807317775196094464 |