Mitochondrial biosynthesis of cholesterol in leydig cells from rat testis

The subcellular location of some enzymes responsible for cholesterol biosynthesis was studied in metrizamide-purified rat Leydig cells.The highest activity of 3-hydroxy-3-methyl glutaryl coenzyme A reductase (HMG-CoA reductase), a key regulatory enzyme in the cholesterol pathway, was associated with...

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Autores principales: Pignataro, O.P., Radicella, J.P., Calvo, J.C., Charreau, E.H.
Formato: JOUR
Materias:
cholesterol biosynthesis
HMG-CoA reductase
Leydig cell
hydroxymethylglutaryl coenzyme a reductase
radioisotope
acetyl coenzyme a c 14
animal experiment
biological model
cholesterol synthesis
endocrine system
leydig cell
male genital system
mitochondrion
nonhuman
rat
Acetates
Acetyl Coenzyme A
Animal
Cell Compartmentation
Cholesterol
Hydroxymethylglutaryl CoA Reductases
Leydig Cells
Male
Mitochondria
Rats
Support, Non-U.S. Gov't
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_03037207_v33_n1_p53_Pignataro
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_03037207_v33_n1_p53_Pignataro
record_format dspace
spelling todo:paper_03037207_v33_n1_p53_Pignataro2023-10-03T15:19:54Z Mitochondrial biosynthesis of cholesterol in leydig cells from rat testis Pignataro, O.P. Radicella, J.P. Calvo, J.C. Charreau, E.H. cholesterol biosynthesis HMG-CoA reductase Leydig cell hydroxymethylglutaryl coenzyme a reductase radioisotope acetyl coenzyme a c 14 animal experiment biological model cholesterol synthesis endocrine system leydig cell male genital system mitochondrion nonhuman rat Acetates Acetyl Coenzyme A Animal Cell Compartmentation Cholesterol Hydroxymethylglutaryl CoA Reductases Leydig Cells Male Mitochondria Rats Support, Non-U.S. Gov't The subcellular location of some enzymes responsible for cholesterol biosynthesis was studied in metrizamide-purified rat Leydig cells.The highest activity of 3-hydroxy-3-methyl glutaryl coenzyme A reductase (HMG-CoA reductase), a key regulatory enzyme in the cholesterol pathway, was associated with highly enriched mitochondrial fractions with recovery of 62% of the total activity and was located on the inner membrane. A significant part of the activity (35%) was also present in the cytoplasm. The activity of this enzyme in the other subcellular fractions was negligible. The HMG-CoA synthase activity was also found almost entirely in the mitochondria (90%). Otherwise no detectable activity of HMG-CoA lyase was present in the subcellular fractions studied. Furthermore, cholesterol may be synthesized from acetyl-CoA inside the mitochondrion, since a significant incorporation (90%) of [14C]acetyl-CoA into digitonin-precipitable sterols was observed in this organelle and only 10% in the cytoplasmic fraction. The evidence strongly suggests that much of the cholesterol biosynthesis that takes place in Leydig cells is carried out within the mitochondria. © 1983. Fil:Pignataro, O.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Radicella, J.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Calvo, J.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Charreau, E.H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03037207_v33_n1_p53_Pignataro
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic cholesterol biosynthesis
HMG-CoA reductase
Leydig cell
hydroxymethylglutaryl coenzyme a reductase
radioisotope
acetyl coenzyme a c 14
animal experiment
biological model
cholesterol synthesis
endocrine system
leydig cell
male genital system
mitochondrion
nonhuman
rat
Acetates
Acetyl Coenzyme A
Animal
Cell Compartmentation
Cholesterol
Hydroxymethylglutaryl CoA Reductases
Leydig Cells
Male
Mitochondria
Rats
Support, Non-U.S. Gov't
spellingShingle cholesterol biosynthesis
HMG-CoA reductase
Leydig cell
hydroxymethylglutaryl coenzyme a reductase
radioisotope
acetyl coenzyme a c 14
animal experiment
biological model
cholesterol synthesis
endocrine system
leydig cell
male genital system
mitochondrion
nonhuman
rat
Acetates
Acetyl Coenzyme A
Animal
Cell Compartmentation
Cholesterol
Hydroxymethylglutaryl CoA Reductases
Leydig Cells
Male
Mitochondria
Rats
Support, Non-U.S. Gov't
Pignataro, O.P.
Radicella, J.P.
Calvo, J.C.
Charreau, E.H.
Mitochondrial biosynthesis of cholesterol in leydig cells from rat testis
topic_facet cholesterol biosynthesis
HMG-CoA reductase
Leydig cell
hydroxymethylglutaryl coenzyme a reductase
radioisotope
acetyl coenzyme a c 14
animal experiment
biological model
cholesterol synthesis
endocrine system
leydig cell
male genital system
mitochondrion
nonhuman
rat
Acetates
Acetyl Coenzyme A
Animal
Cell Compartmentation
Cholesterol
Hydroxymethylglutaryl CoA Reductases
Leydig Cells
Male
Mitochondria
Rats
Support, Non-U.S. Gov't
description The subcellular location of some enzymes responsible for cholesterol biosynthesis was studied in metrizamide-purified rat Leydig cells.The highest activity of 3-hydroxy-3-methyl glutaryl coenzyme A reductase (HMG-CoA reductase), a key regulatory enzyme in the cholesterol pathway, was associated with highly enriched mitochondrial fractions with recovery of 62% of the total activity and was located on the inner membrane. A significant part of the activity (35%) was also present in the cytoplasm. The activity of this enzyme in the other subcellular fractions was negligible. The HMG-CoA synthase activity was also found almost entirely in the mitochondria (90%). Otherwise no detectable activity of HMG-CoA lyase was present in the subcellular fractions studied. Furthermore, cholesterol may be synthesized from acetyl-CoA inside the mitochondrion, since a significant incorporation (90%) of [14C]acetyl-CoA into digitonin-precipitable sterols was observed in this organelle and only 10% in the cytoplasmic fraction. The evidence strongly suggests that much of the cholesterol biosynthesis that takes place in Leydig cells is carried out within the mitochondria. © 1983.
format JOUR
author Pignataro, O.P.
Radicella, J.P.
Calvo, J.C.
Charreau, E.H.
author_facet Pignataro, O.P.
Radicella, J.P.
Calvo, J.C.
Charreau, E.H.
author_sort Pignataro, O.P.
title Mitochondrial biosynthesis of cholesterol in leydig cells from rat testis
title_short Mitochondrial biosynthesis of cholesterol in leydig cells from rat testis
title_full Mitochondrial biosynthesis of cholesterol in leydig cells from rat testis
title_fullStr Mitochondrial biosynthesis of cholesterol in leydig cells from rat testis
title_full_unstemmed Mitochondrial biosynthesis of cholesterol in leydig cells from rat testis
title_sort mitochondrial biosynthesis of cholesterol in leydig cells from rat testis
url http://hdl.handle.net/20.500.12110/paper_03037207_v33_n1_p53_Pignataro
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AT radicellajp mitochondrialbiosynthesisofcholesterolinleydigcellsfromrattestis
AT calvojc mitochondrialbiosynthesisofcholesterolinleydigcellsfromrattestis
AT charreaueh mitochondrialbiosynthesisofcholesterolinleydigcellsfromrattestis
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