Modulation of macrophage inflammatory profile in pregnant nonobese diabetic (NOD) mice

During normal early pregnancy circulating monocytes are recruited to the maternal-placental interface where they differentiate to macrophages expressing different functional phenotypes for the maintenance of tissue homeostasis. Pregnancy in the nonobese diabetic (NOD) mouse model presents some patho...

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Autores principales:	Larocca, L., Hauk, V., Calafat, M., Roca, V., Fraccaroli, L., Franchi, A., Ramhorst, R., Leirós, C.P.
Formato:	JOUR
Materias:	Macrophage phenotypes NOD mice Phagocytosis Pregnancy VIP gamma interferon immunoglobulin enhancer binding protein interleukin 10 interleukin 12 synaptotagmin I vasoactive intestinal polypeptide animal cell animal experiment apoptosis article cell stimulation cellular distribution controlled study cytokine response female first trimester pregnancy mouse nonhuman peritoneum macrophage phagocyte phagocytosis phenotype pregnancy priority journal protein expression protein function Animals Apoptosis Coculture Techniques Female Inflammation Kinetics Lipopolysaccharides Macrophage Activation Macrophages Mice Mice, Inbred BALB C Mice, Inbred NOD NF-kappa B
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_03037207_v333_n2_p112_Larocca
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_03037207_v333_n2_p112_Larocca2023-10-03T15:19:53Z Modulation of macrophage inflammatory profile in pregnant nonobese diabetic (NOD) mice Larocca, L. Hauk, V. Calafat, M. Roca, V. Fraccaroli, L. Franchi, A. Ramhorst, R. Leirós, C.P. Macrophage phenotypes NOD mice Phagocytosis Pregnancy VIP gamma interferon immunoglobulin enhancer binding protein interleukin 10 interleukin 12 synaptotagmin I vasoactive intestinal polypeptide animal cell animal experiment apoptosis article cell stimulation cellular distribution controlled study cytokine response female first trimester pregnancy mouse nonhuman peritoneum macrophage phagocyte phagocytosis phenotype pregnancy priority journal protein expression protein function Animals Apoptosis Coculture Techniques Female Inflammation Kinetics Lipopolysaccharides Macrophage Activation Macrophages Mice Mice, Inbred BALB C Mice, Inbred NOD NF-kappa B Phagocytosis Pregnancy During normal early pregnancy circulating monocytes are recruited to the maternal-placental interface where they differentiate to macrophages expressing different functional phenotypes for the maintenance of tissue homeostasis. Pregnancy in the nonobese diabetic (NOD) mouse model presents some pathological features in the pre-diabetic stage. The aim of this work was to analyze the functional profile of peritoneal macrophages faced with inflammatory and phagocytic stimuli in early pregnant pre-diabetic NOD mice and their modulation by vasoactive intestinal peptide (VIP). Pregnant NOD mouse macrophages showed no basal NFκB activation, lower IL-12 and nitrites production compared with the macrophages from non-pregnant NOD mice. Their pro-inflammatory aberrant response to LPS and apoptotic cell challenge was reduced and VIP inhibited macrophage residual deleterious responses to apoptotic cells. A functional phenotype switch in macrophages during pregnancy in NOD mice and a promoting effect of VIP towards this regulatory phenotype would be in line with the central role of macrophages in the maternal-placental dialogue. © 2010 Elsevier Ireland Ltd. Fil:Larocca, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Hauk, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Calafat, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Roca, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Fraccaroli, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Franchi, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ramhorst, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Leirós, C.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03037207_v333_n2_p112_Larocca
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Macrophage phenotypes NOD mice Phagocytosis Pregnancy VIP gamma interferon immunoglobulin enhancer binding protein interleukin 10 interleukin 12 synaptotagmin I vasoactive intestinal polypeptide animal cell animal experiment apoptosis article cell stimulation cellular distribution controlled study cytokine response female first trimester pregnancy mouse nonhuman peritoneum macrophage phagocyte phagocytosis phenotype pregnancy priority journal protein expression protein function Animals Apoptosis Coculture Techniques Female Inflammation Kinetics Lipopolysaccharides Macrophage Activation Macrophages Mice Mice, Inbred BALB C Mice, Inbred NOD NF-kappa B Phagocytosis Pregnancy
spellingShingle	Macrophage phenotypes NOD mice Phagocytosis Pregnancy VIP gamma interferon immunoglobulin enhancer binding protein interleukin 10 interleukin 12 synaptotagmin I vasoactive intestinal polypeptide animal cell animal experiment apoptosis article cell stimulation cellular distribution controlled study cytokine response female first trimester pregnancy mouse nonhuman peritoneum macrophage phagocyte phagocytosis phenotype pregnancy priority journal protein expression protein function Animals Apoptosis Coculture Techniques Female Inflammation Kinetics Lipopolysaccharides Macrophage Activation Macrophages Mice Mice, Inbred BALB C Mice, Inbred NOD NF-kappa B Phagocytosis Pregnancy Larocca, L. Hauk, V. Calafat, M. Roca, V. Fraccaroli, L. Franchi, A. Ramhorst, R. Leirós, C.P. Modulation of macrophage inflammatory profile in pregnant nonobese diabetic (NOD) mice
topic_facet	Macrophage phenotypes NOD mice Phagocytosis Pregnancy VIP gamma interferon immunoglobulin enhancer binding protein interleukin 10 interleukin 12 synaptotagmin I vasoactive intestinal polypeptide animal cell animal experiment apoptosis article cell stimulation cellular distribution controlled study cytokine response female first trimester pregnancy mouse nonhuman peritoneum macrophage phagocyte phagocytosis phenotype pregnancy priority journal protein expression protein function Animals Apoptosis Coculture Techniques Female Inflammation Kinetics Lipopolysaccharides Macrophage Activation Macrophages Mice Mice, Inbred BALB C Mice, Inbred NOD NF-kappa B Phagocytosis Pregnancy
description	During normal early pregnancy circulating monocytes are recruited to the maternal-placental interface where they differentiate to macrophages expressing different functional phenotypes for the maintenance of tissue homeostasis. Pregnancy in the nonobese diabetic (NOD) mouse model presents some pathological features in the pre-diabetic stage. The aim of this work was to analyze the functional profile of peritoneal macrophages faced with inflammatory and phagocytic stimuli in early pregnant pre-diabetic NOD mice and their modulation by vasoactive intestinal peptide (VIP). Pregnant NOD mouse macrophages showed no basal NFκB activation, lower IL-12 and nitrites production compared with the macrophages from non-pregnant NOD mice. Their pro-inflammatory aberrant response to LPS and apoptotic cell challenge was reduced and VIP inhibited macrophage residual deleterious responses to apoptotic cells. A functional phenotype switch in macrophages during pregnancy in NOD mice and a promoting effect of VIP towards this regulatory phenotype would be in line with the central role of macrophages in the maternal-placental dialogue. © 2010 Elsevier Ireland Ltd.
format	JOUR
author	Larocca, L. Hauk, V. Calafat, M. Roca, V. Fraccaroli, L. Franchi, A. Ramhorst, R. Leirós, C.P.
author_facet	Larocca, L. Hauk, V. Calafat, M. Roca, V. Fraccaroli, L. Franchi, A. Ramhorst, R. Leirós, C.P.
author_sort	Larocca, L.
title	Modulation of macrophage inflammatory profile in pregnant nonobese diabetic (NOD) mice
title_short	Modulation of macrophage inflammatory profile in pregnant nonobese diabetic (NOD) mice
title_full	Modulation of macrophage inflammatory profile in pregnant nonobese diabetic (NOD) mice
title_fullStr	Modulation of macrophage inflammatory profile in pregnant nonobese diabetic (NOD) mice
title_full_unstemmed	Modulation of macrophage inflammatory profile in pregnant nonobese diabetic (NOD) mice
title_sort	modulation of macrophage inflammatory profile in pregnant nonobese diabetic (nod) mice
url	http://hdl.handle.net/20.500.12110/paper_03037207_v333_n2_p112_Larocca
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Modulation of macrophage inflammatory profile in pregnant nonobese diabetic (NOD) mice

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