Anti-SOS effects induced in Bacillus subtilis by a φ{symbol}105 mutant prophage
The presence of the mutant prophage φ{symbol}105cts23 in Bacillus subtilis strains strongly affected several biological parameters including the viability of protoplasts and the establishment of plasmid pC194. A defective inducibility of the prophage after treatments that de-repress the SOS-like res...
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todo:paper_03028933_v160_n6_p486_Rubinstein2023-10-03T15:18:43Z Anti-SOS effects induced in Bacillus subtilis by a φ{symbol}105 mutant prophage Rubinstein, C.P. Coso, O.A. Ruzal, S. Sanchez-Rivas, C. Bacillus subtilis Bacteriophage φ{symbol}105 Mutant repressor Recombination SOS effects article bacillus subtilis bacteriophage nonhuman osmotic stress priority journal prophage stress virus mutant Bacillus Phages Bacillus subtilis Mutation Plasmids Protoplasts Recombination, Genetic SOS Response (Genetics) Support, Non-U.S. Gov't Transformation, Bacterial Bacillus subtilis The presence of the mutant prophage φ{symbol}105cts23 in Bacillus subtilis strains strongly affected several biological parameters including the viability of protoplasts and the establishment of plasmid pC194. A defective inducibility of the prophage after treatments that de-repress the SOS-like response were also observed. Although these alterations suggested a Rec-deficient phenotype, homologous recombination was not impaired in these lysogenic derivatives. In fact, chromosomal DNA transformation in these competent cells was more efficient than in cells carrying the wild type prophage: cell death due to prophage induction upon competence development was lower than expected. Alterations in the response to SOS-inducing agents and to osmotic stress correlated with the presence of this particular mutant prophage or the cloned thermosensitive repressor at the permissive temperature. The induction of an anti-SOS effect is discussed. © 1993 Springer-Verlag. Fil:Rubinstein, C.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Coso, O.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ruzal, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03028933_v160_n6_p486_Rubinstein |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Bacillus subtilis Bacteriophage φ{symbol}105 Mutant repressor Recombination SOS effects article bacillus subtilis bacteriophage nonhuman osmotic stress priority journal prophage stress virus mutant Bacillus Phages Bacillus subtilis Mutation Plasmids Protoplasts Recombination, Genetic SOS Response (Genetics) Support, Non-U.S. Gov't Transformation, Bacterial Bacillus subtilis |
| spellingShingle |
Bacillus subtilis Bacteriophage φ{symbol}105 Mutant repressor Recombination SOS effects article bacillus subtilis bacteriophage nonhuman osmotic stress priority journal prophage stress virus mutant Bacillus Phages Bacillus subtilis Mutation Plasmids Protoplasts Recombination, Genetic SOS Response (Genetics) Support, Non-U.S. Gov't Transformation, Bacterial Bacillus subtilis Rubinstein, C.P. Coso, O.A. Ruzal, S. Sanchez-Rivas, C. Anti-SOS effects induced in Bacillus subtilis by a φ{symbol}105 mutant prophage |
| topic_facet |
Bacillus subtilis Bacteriophage φ{symbol}105 Mutant repressor Recombination SOS effects article bacillus subtilis bacteriophage nonhuman osmotic stress priority journal prophage stress virus mutant Bacillus Phages Bacillus subtilis Mutation Plasmids Protoplasts Recombination, Genetic SOS Response (Genetics) Support, Non-U.S. Gov't Transformation, Bacterial Bacillus subtilis |
| description |
The presence of the mutant prophage φ{symbol}105cts23 in Bacillus subtilis strains strongly affected several biological parameters including the viability of protoplasts and the establishment of plasmid pC194. A defective inducibility of the prophage after treatments that de-repress the SOS-like response were also observed. Although these alterations suggested a Rec-deficient phenotype, homologous recombination was not impaired in these lysogenic derivatives. In fact, chromosomal DNA transformation in these competent cells was more efficient than in cells carrying the wild type prophage: cell death due to prophage induction upon competence development was lower than expected. Alterations in the response to SOS-inducing agents and to osmotic stress correlated with the presence of this particular mutant prophage or the cloned thermosensitive repressor at the permissive temperature. The induction of an anti-SOS effect is discussed. © 1993 Springer-Verlag. |
| format |
JOUR |
| author |
Rubinstein, C.P. Coso, O.A. Ruzal, S. Sanchez-Rivas, C. |
| author_facet |
Rubinstein, C.P. Coso, O.A. Ruzal, S. Sanchez-Rivas, C. |
| author_sort |
Rubinstein, C.P. |
| title |
Anti-SOS effects induced in Bacillus subtilis by a φ{symbol}105 mutant prophage |
| title_short |
Anti-SOS effects induced in Bacillus subtilis by a φ{symbol}105 mutant prophage |
| title_full |
Anti-SOS effects induced in Bacillus subtilis by a φ{symbol}105 mutant prophage |
| title_fullStr |
Anti-SOS effects induced in Bacillus subtilis by a φ{symbol}105 mutant prophage |
| title_full_unstemmed |
Anti-SOS effects induced in Bacillus subtilis by a φ{symbol}105 mutant prophage |
| title_sort |
anti-sos effects induced in bacillus subtilis by a φ{symbol}105 mutant prophage |
| url |
http://hdl.handle.net/20.500.12110/paper_03028933_v160_n6_p486_Rubinstein |
| work_keys_str_mv |
AT rubinsteincp antisoseffectsinducedinbacillussubtilisbyaphsymbol105mutantprophage AT cosooa antisoseffectsinducedinbacillussubtilisbyaphsymbol105mutantprophage AT ruzals antisoseffectsinducedinbacillussubtilisbyaphsymbol105mutantprophage AT sanchezrivasc antisoseffectsinducedinbacillussubtilisbyaphsymbol105mutantprophage |
| _version_ |
1807317634088173568 |