Biosynthesis of cyclic β-(1-3),β-(1-6) glucan in Bradyrhizobium spp.
Inner membranes of Bradyrhizobium japonicum strain USDA 110 produced in vitro soluble and insoluble β-(1-3),β-(1-6) glucansA preliminary account of this work was presented at the 5th International Symposium on the Molecular Genetics of Plant-Microbe Interactions, Interlaken, Switzerland, September 1...
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todo:paper_03028933_v159_n1_p30_InondeIannino2023-10-03T15:18:43Z Biosynthesis of cyclic β-(1-3),β-(1-6) glucan in Bradyrhizobium spp. Iñón de Iannino, N. Ugalde, R.A. Bradyrhizobium japonicum Bradyrhizobium spp. Cyclic β-(1-3),β-(1-6) glucan Glucan Laminarin Protein intermediate glucan laminaran article bacterial membrane biosynthesis bradyrhizobium nonhuman priority journal Cell Membrane Glucans Membrane Proteins Rhizobiaceae Support, Non-U.S. Gov't Agrobacterium tumefaciens Bacteria (microorganisms) Bradyrhizobium Bradyrhizobium japonicum Bradyrhizobium japonicum USDA 110 Mesorhizobium loti Rhizobium Sinorhizobium meliloti Inner membranes of Bradyrhizobium japonicum strain USDA 110 produced in vitro soluble and insoluble β-(1-3),β-(1-6) glucansA preliminary account of this work was presented at the 5th International Symposium on the Molecular Genetics of Plant-Microbe Interactions, Interlaken, Switzerland, September 1990. The reaction proceeded through a 90 kDa inner membrane intermediate protein; used UDP-glucose as sugar donor and required Mg2+. Gel chromatography of soluble glucans resolved a cyclic β-(1-3) glucan with a degree of polymerization of eleven from a family of β-(1-3),β-(1-6) glucans with variable degree of polymerization higher than eleven. Bradyrhizobium strains BR4406 and BR8404 isolated from tree legume nodules in Southeast Brazil produce β-(1-3),β-(1-6) glucans very similar to that of B. japonicum. A 100 kDa protein was identified in these strains as intermediates in the synthesis of these glucans. Inner membranes of B. japonicum USDA110, B. japonicum I17, and Bradyrhizobium strains BR4406 and BR8404 incubated with UDP-glucose were unable to synthesize β-(1-2) glucan and lacked the 235 kDa intermediate protein known to be involved in the synthesis of β-(1-2) glucan in Agrobacterium tumefaciens, Rhizobium meliloti and Rhizobium loti. © 1993 Springer-Verlag. Fil:Ugalde, R.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03028933_v159_n1_p30_InondeIannino |
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Universidad de Buenos Aires |
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I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Bradyrhizobium japonicum Bradyrhizobium spp. Cyclic β-(1-3),β-(1-6) glucan Glucan Laminarin Protein intermediate glucan laminaran article bacterial membrane biosynthesis bradyrhizobium nonhuman priority journal Cell Membrane Glucans Membrane Proteins Rhizobiaceae Support, Non-U.S. Gov't Agrobacterium tumefaciens Bacteria (microorganisms) Bradyrhizobium Bradyrhizobium japonicum Bradyrhizobium japonicum USDA 110 Mesorhizobium loti Rhizobium Sinorhizobium meliloti |
| spellingShingle |
Bradyrhizobium japonicum Bradyrhizobium spp. Cyclic β-(1-3),β-(1-6) glucan Glucan Laminarin Protein intermediate glucan laminaran article bacterial membrane biosynthesis bradyrhizobium nonhuman priority journal Cell Membrane Glucans Membrane Proteins Rhizobiaceae Support, Non-U.S. Gov't Agrobacterium tumefaciens Bacteria (microorganisms) Bradyrhizobium Bradyrhizobium japonicum Bradyrhizobium japonicum USDA 110 Mesorhizobium loti Rhizobium Sinorhizobium meliloti Iñón de Iannino, N. Ugalde, R.A. Biosynthesis of cyclic β-(1-3),β-(1-6) glucan in Bradyrhizobium spp. |
| topic_facet |
Bradyrhizobium japonicum Bradyrhizobium spp. Cyclic β-(1-3),β-(1-6) glucan Glucan Laminarin Protein intermediate glucan laminaran article bacterial membrane biosynthesis bradyrhizobium nonhuman priority journal Cell Membrane Glucans Membrane Proteins Rhizobiaceae Support, Non-U.S. Gov't Agrobacterium tumefaciens Bacteria (microorganisms) Bradyrhizobium Bradyrhizobium japonicum Bradyrhizobium japonicum USDA 110 Mesorhizobium loti Rhizobium Sinorhizobium meliloti |
| description |
Inner membranes of Bradyrhizobium japonicum strain USDA 110 produced in vitro soluble and insoluble β-(1-3),β-(1-6) glucansA preliminary account of this work was presented at the 5th International Symposium on the Molecular Genetics of Plant-Microbe Interactions, Interlaken, Switzerland, September 1990. The reaction proceeded through a 90 kDa inner membrane intermediate protein; used UDP-glucose as sugar donor and required Mg2+. Gel chromatography of soluble glucans resolved a cyclic β-(1-3) glucan with a degree of polymerization of eleven from a family of β-(1-3),β-(1-6) glucans with variable degree of polymerization higher than eleven. Bradyrhizobium strains BR4406 and BR8404 isolated from tree legume nodules in Southeast Brazil produce β-(1-3),β-(1-6) glucans very similar to that of B. japonicum. A 100 kDa protein was identified in these strains as intermediates in the synthesis of these glucans. Inner membranes of B. japonicum USDA110, B. japonicum I17, and Bradyrhizobium strains BR4406 and BR8404 incubated with UDP-glucose were unable to synthesize β-(1-2) glucan and lacked the 235 kDa intermediate protein known to be involved in the synthesis of β-(1-2) glucan in Agrobacterium tumefaciens, Rhizobium meliloti and Rhizobium loti. © 1993 Springer-Verlag. |
| format |
JOUR |
| author |
Iñón de Iannino, N. Ugalde, R.A. |
| author_facet |
Iñón de Iannino, N. Ugalde, R.A. |
| author_sort |
Iñón de Iannino, N. |
| title |
Biosynthesis of cyclic β-(1-3),β-(1-6) glucan in Bradyrhizobium spp. |
| title_short |
Biosynthesis of cyclic β-(1-3),β-(1-6) glucan in Bradyrhizobium spp. |
| title_full |
Biosynthesis of cyclic β-(1-3),β-(1-6) glucan in Bradyrhizobium spp. |
| title_fullStr |
Biosynthesis of cyclic β-(1-3),β-(1-6) glucan in Bradyrhizobium spp. |
| title_full_unstemmed |
Biosynthesis of cyclic β-(1-3),β-(1-6) glucan in Bradyrhizobium spp. |
| title_sort |
biosynthesis of cyclic β-(1-3),β-(1-6) glucan in bradyrhizobium spp. |
| url |
http://hdl.handle.net/20.500.12110/paper_03028933_v159_n1_p30_InondeIannino |
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AT inondeianninon biosynthesisofcyclicb13b16glucaninbradyrhizobiumspp AT ugaldera biosynthesisofcyclicb13b16glucaninbradyrhizobiumspp |
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1782026845980459008 |