Protective effect of vanilloids against chemical stress on the white-rot fungus Ganoderma lucidum
Bioremediation of contaminated sites by biosorption of pollutants onto a wide range of materials has emerged as a promising treatment for recalcitrant aromatic compounds or heavy metals. When adsorption occurs on living white-rot fungi mycelia, the pollutants may be degraded by ligninolytic enzymes....
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todo:paper_03014797_v124_n_p1_Kuhar2023-10-03T15:18:23Z Protective effect of vanilloids against chemical stress on the white-rot fungus Ganoderma lucidum Kuhar, F. Papinutti, L. Bioremediation Biosorption Exopolysaccharide Fungal growth Ligninolytic enzymes White rot fungi cadmium clotrimazole crystal violet guaiacol malachite green vanillic acid benzaldehyde derivative coumaric acid ferulic acid fungicide pollutant vanillic acid vanillin adsorption biodegradation bioremediation cadmium concentration (composition) contaminated land enzyme activity fungus lignin organic acid polysaccharide toxicity adsorption antifungal resistance article bioremediation biosorption chemical stress concentration (parameters) controlled study Ganoderma lucidum liquid culture nonhuman oxidation protection drug effects Ganoderma lucidum growth, development and aging kinetics metabolism pollutant toxicity Fungi Ganoderma lucidum Gentiana Adsorption Benzaldehydes Biodegradation, Environmental Coumaric Acids Environmental Pollutants Fungicides, Industrial Kinetics Reishi Vanillic Acid Bioremediation of contaminated sites by biosorption of pollutants onto a wide range of materials has emerged as a promising treatment for recalcitrant aromatic compounds or heavy metals. When adsorption occurs on living white-rot fungi mycelia, the pollutants may be degraded by ligninolytic enzymes. However, the survival of mycelia in harsh conditions is one of the drawbacks of those methodologies. In this study, it was demonstrated that culture media supplemented with several guaiacol derivatives (vanilloids) increased the resistance of Ganoderma lucidum E47 cultures to chemical stress by enhancing the adsorptive capacity of the extracellular mucilaginous material (ECMM). The toxicity of the fungicides gentian violet (GV), malachite green (MG) and clotrimazole, and the heavy metal Cadmium was noticeably diminished in fungal cultures supplemented with the guaiacol derivative vanillic acid (VA). No degradation of the tested compounds was detected. The activity of the oxidative enzymatic systems like laccase, a well-known oxidase associated to dye degradation, was only detectable after complete growth on plates. Extremely low concentrations of VA caused a significant protective effect, radial extension of the growth halo in plates supplemented with 0.0001mM of VA plus GV was up to 20% to that obtained in control plates (without addition of GV and VA). Therefore, the protective effect could not be attributable to VA per se. ECMM separated from the mycelium exhibited a much higher increase in the adsorptive capacity when isolated from liquid cultures containing VA, while that obtained from unsupplemented cultures showed an almost null adsorptive capacity. © 2013 Elsevier Ltd. Fil:Kuhar, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Papinutti, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03014797_v124_n_p1_Kuhar |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Bioremediation Biosorption Exopolysaccharide Fungal growth Ligninolytic enzymes White rot fungi cadmium clotrimazole crystal violet guaiacol malachite green vanillic acid benzaldehyde derivative coumaric acid ferulic acid fungicide pollutant vanillic acid vanillin adsorption biodegradation bioremediation cadmium concentration (composition) contaminated land enzyme activity fungus lignin organic acid polysaccharide toxicity adsorption antifungal resistance article bioremediation biosorption chemical stress concentration (parameters) controlled study Ganoderma lucidum liquid culture nonhuman oxidation protection drug effects Ganoderma lucidum growth, development and aging kinetics metabolism pollutant toxicity Fungi Ganoderma lucidum Gentiana Adsorption Benzaldehydes Biodegradation, Environmental Coumaric Acids Environmental Pollutants Fungicides, Industrial Kinetics Reishi Vanillic Acid |
| spellingShingle |
Bioremediation Biosorption Exopolysaccharide Fungal growth Ligninolytic enzymes White rot fungi cadmium clotrimazole crystal violet guaiacol malachite green vanillic acid benzaldehyde derivative coumaric acid ferulic acid fungicide pollutant vanillic acid vanillin adsorption biodegradation bioremediation cadmium concentration (composition) contaminated land enzyme activity fungus lignin organic acid polysaccharide toxicity adsorption antifungal resistance article bioremediation biosorption chemical stress concentration (parameters) controlled study Ganoderma lucidum liquid culture nonhuman oxidation protection drug effects Ganoderma lucidum growth, development and aging kinetics metabolism pollutant toxicity Fungi Ganoderma lucidum Gentiana Adsorption Benzaldehydes Biodegradation, Environmental Coumaric Acids Environmental Pollutants Fungicides, Industrial Kinetics Reishi Vanillic Acid Kuhar, F. Papinutti, L. Protective effect of vanilloids against chemical stress on the white-rot fungus Ganoderma lucidum |
| topic_facet |
Bioremediation Biosorption Exopolysaccharide Fungal growth Ligninolytic enzymes White rot fungi cadmium clotrimazole crystal violet guaiacol malachite green vanillic acid benzaldehyde derivative coumaric acid ferulic acid fungicide pollutant vanillic acid vanillin adsorption biodegradation bioremediation cadmium concentration (composition) contaminated land enzyme activity fungus lignin organic acid polysaccharide toxicity adsorption antifungal resistance article bioremediation biosorption chemical stress concentration (parameters) controlled study Ganoderma lucidum liquid culture nonhuman oxidation protection drug effects Ganoderma lucidum growth, development and aging kinetics metabolism pollutant toxicity Fungi Ganoderma lucidum Gentiana Adsorption Benzaldehydes Biodegradation, Environmental Coumaric Acids Environmental Pollutants Fungicides, Industrial Kinetics Reishi Vanillic Acid |
| description |
Bioremediation of contaminated sites by biosorption of pollutants onto a wide range of materials has emerged as a promising treatment for recalcitrant aromatic compounds or heavy metals. When adsorption occurs on living white-rot fungi mycelia, the pollutants may be degraded by ligninolytic enzymes. However, the survival of mycelia in harsh conditions is one of the drawbacks of those methodologies. In this study, it was demonstrated that culture media supplemented with several guaiacol derivatives (vanilloids) increased the resistance of Ganoderma lucidum E47 cultures to chemical stress by enhancing the adsorptive capacity of the extracellular mucilaginous material (ECMM). The toxicity of the fungicides gentian violet (GV), malachite green (MG) and clotrimazole, and the heavy metal Cadmium was noticeably diminished in fungal cultures supplemented with the guaiacol derivative vanillic acid (VA). No degradation of the tested compounds was detected. The activity of the oxidative enzymatic systems like laccase, a well-known oxidase associated to dye degradation, was only detectable after complete growth on plates. Extremely low concentrations of VA caused a significant protective effect, radial extension of the growth halo in plates supplemented with 0.0001mM of VA plus GV was up to 20% to that obtained in control plates (without addition of GV and VA). Therefore, the protective effect could not be attributable to VA per se. ECMM separated from the mycelium exhibited a much higher increase in the adsorptive capacity when isolated from liquid cultures containing VA, while that obtained from unsupplemented cultures showed an almost null adsorptive capacity. © 2013 Elsevier Ltd. |
| format |
JOUR |
| author |
Kuhar, F. Papinutti, L. |
| author_facet |
Kuhar, F. Papinutti, L. |
| author_sort |
Kuhar, F. |
| title |
Protective effect of vanilloids against chemical stress on the white-rot fungus Ganoderma lucidum |
| title_short |
Protective effect of vanilloids against chemical stress on the white-rot fungus Ganoderma lucidum |
| title_full |
Protective effect of vanilloids against chemical stress on the white-rot fungus Ganoderma lucidum |
| title_fullStr |
Protective effect of vanilloids against chemical stress on the white-rot fungus Ganoderma lucidum |
| title_full_unstemmed |
Protective effect of vanilloids against chemical stress on the white-rot fungus Ganoderma lucidum |
| title_sort |
protective effect of vanilloids against chemical stress on the white-rot fungus ganoderma lucidum |
| url |
http://hdl.handle.net/20.500.12110/paper_03014797_v124_n_p1_Kuhar |
| work_keys_str_mv |
AT kuharf protectiveeffectofvanilloidsagainstchemicalstressonthewhiterotfungusganodermalucidum AT papinuttil protectiveeffectofvanilloidsagainstchemicalstressonthewhiterotfungusganodermalucidum |
| _version_ |
1782024212889731072 |