Effect of culture supernatants from two cancer cell lines on healthy donors' monocytes

Background: it is well known that culture cells secrete to their medium different factors that can alter their own as well as other cells' metabolism and functions. In the present study we evaluated the effect of culture supernatants originated from two cancer cell lines, Calu-1 (lung epidermoi...

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Autores principales: Novellino, P.S., Trejo, Y.G., Beviacqua, M., Bordenave, R.H., Rumi, L.S.
Formato: JOUR
Materias:
Culture supernatants
Lung cancer cell lines
Monocytes
HLA DR antigen
article
cell culture
culture medium
drug effect
fluorescent antibody technique
human
monocyte
phagocytosis
secretion
Culture Media, Conditioned
Fluorescent Antibody Technique, Indirect
HLA-DR Antigens
Humans
Phagocytosis
Tumor Cells, Cultured
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_03010546_v27_n4_p195_Novellino
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_03010546_v27_n4_p195_Novellino
record_format dspace
spelling todo:paper_03010546_v27_n4_p195_Novellino2023-10-03T15:18:17Z Effect of culture supernatants from two cancer cell lines on healthy donors' monocytes Novellino, P.S. Trejo, Y.G. Beviacqua, M. Bordenave, R.H. Rumi, L.S. Culture supernatants Lung cancer cell lines Monocytes HLA DR antigen article cell culture culture medium drug effect fluorescent antibody technique human monocyte phagocytosis secretion Culture Media, Conditioned Fluorescent Antibody Technique, Indirect HLA-DR Antigens Humans Monocytes Phagocytosis Tumor Cells, Cultured Background: it is well known that culture cells secrete to their medium different factors that can alter their own as well as other cells' metabolism and functions. In the present study we evaluated the effect of culture supernatants originated from two cancer cell lines, Calu-1 (lung epidermoid carcinoma) and A-427 (lung adenocarcinoma) on peripheral blood monocytes (MO) from 20 healthy donors (HD). Methods and results: MO were incubated with supernatants or with medium only (controls) during 2 or 18 hours. There were determined the expression of HLA-DR antigen by indirect immunofluorescence technique, the formation of reactive oxygen intermediates (NBT reduction capacity) and the phagocytic capacity (Candida albicans-anti Candida albicans system). These determinations were made also in MO from 16 patients with advanced lung cancer (LCP). Percentages of expression (media ± SE) were: HLA-DR (+) MO: Calu-1 = 30 ± 2; A-427 = 31 ± 2; LCP = 52 ± 3 (p < 0.0001 vs HD: 77 ± 1%) NBT (+)MO: Calu-1 = 47 ± 1; A-427 = 49 ± 1; LCP = 56 ± 2 (p < 0.01 vs HD: 45 ± 2%), Phagocytic MO: Calu-1 = 38 ± 3; A-427 = 39 ± 2; LCP = 32 ± 3 (p < 0.0001 vs HD: 50 ± 1%). Conclusions: we conclude that this in vitro model reproduced characteristics found in MO from lung cancer patients, since culture supernatants induced in normal MO phenotypic and functional characteristics also found in MO from patients analyzed. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03010546_v27_n4_p195_Novellino
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Culture supernatants
Lung cancer cell lines
Monocytes
HLA DR antigen
article
cell culture
culture medium
drug effect
fluorescent antibody technique
human
monocyte
phagocytosis
secretion
Culture Media, Conditioned
Fluorescent Antibody Technique, Indirect
HLA-DR Antigens
Humans
Monocytes
Phagocytosis
Tumor Cells, Cultured
spellingShingle Culture supernatants
Lung cancer cell lines
Monocytes
HLA DR antigen
article
cell culture
culture medium
drug effect
fluorescent antibody technique
human
monocyte
phagocytosis
secretion
Culture Media, Conditioned
Fluorescent Antibody Technique, Indirect
HLA-DR Antigens
Humans
Monocytes
Phagocytosis
Tumor Cells, Cultured
Novellino, P.S.
Trejo, Y.G.
Beviacqua, M.
Bordenave, R.H.
Rumi, L.S.
Effect of culture supernatants from two cancer cell lines on healthy donors' monocytes
topic_facet Culture supernatants
Lung cancer cell lines
Monocytes
HLA DR antigen
article
cell culture
culture medium
drug effect
fluorescent antibody technique
human
monocyte
phagocytosis
secretion
Culture Media, Conditioned
Fluorescent Antibody Technique, Indirect
HLA-DR Antigens
Humans
Monocytes
Phagocytosis
Tumor Cells, Cultured
description Background: it is well known that culture cells secrete to their medium different factors that can alter their own as well as other cells' metabolism and functions. In the present study we evaluated the effect of culture supernatants originated from two cancer cell lines, Calu-1 (lung epidermoid carcinoma) and A-427 (lung adenocarcinoma) on peripheral blood monocytes (MO) from 20 healthy donors (HD). Methods and results: MO were incubated with supernatants or with medium only (controls) during 2 or 18 hours. There were determined the expression of HLA-DR antigen by indirect immunofluorescence technique, the formation of reactive oxygen intermediates (NBT reduction capacity) and the phagocytic capacity (Candida albicans-anti Candida albicans system). These determinations were made also in MO from 16 patients with advanced lung cancer (LCP). Percentages of expression (media ± SE) were: HLA-DR (+) MO: Calu-1 = 30 ± 2; A-427 = 31 ± 2; LCP = 52 ± 3 (p < 0.0001 vs HD: 77 ± 1%) NBT (+)MO: Calu-1 = 47 ± 1; A-427 = 49 ± 1; LCP = 56 ± 2 (p < 0.01 vs HD: 45 ± 2%), Phagocytic MO: Calu-1 = 38 ± 3; A-427 = 39 ± 2; LCP = 32 ± 3 (p < 0.0001 vs HD: 50 ± 1%). Conclusions: we conclude that this in vitro model reproduced characteristics found in MO from lung cancer patients, since culture supernatants induced in normal MO phenotypic and functional characteristics also found in MO from patients analyzed.
format JOUR
author Novellino, P.S.
Trejo, Y.G.
Beviacqua, M.
Bordenave, R.H.
Rumi, L.S.
author_facet Novellino, P.S.
Trejo, Y.G.
Beviacqua, M.
Bordenave, R.H.
Rumi, L.S.
author_sort Novellino, P.S.
title Effect of culture supernatants from two cancer cell lines on healthy donors' monocytes
title_short Effect of culture supernatants from two cancer cell lines on healthy donors' monocytes
title_full Effect of culture supernatants from two cancer cell lines on healthy donors' monocytes
title_fullStr Effect of culture supernatants from two cancer cell lines on healthy donors' monocytes
title_full_unstemmed Effect of culture supernatants from two cancer cell lines on healthy donors' monocytes
title_sort effect of culture supernatants from two cancer cell lines on healthy donors' monocytes
url http://hdl.handle.net/20.500.12110/paper_03010546_v27_n4_p195_Novellino
work_keys_str_mv AT novellinops effectofculturesupernatantsfromtwocancercelllinesonhealthydonorsmonocytes
AT trejoyg effectofculturesupernatantsfromtwocancercelllinesonhealthydonorsmonocytes
AT beviacquam effectofculturesupernatantsfromtwocancercelllinesonhealthydonorsmonocytes
AT bordenaverh effectofculturesupernatantsfromtwocancercelllinesonhealthydonorsmonocytes
AT rumils effectofculturesupernatantsfromtwocancercelllinesonhealthydonorsmonocytes
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