Insulin-like growth factor-1 receptor regulation in activated human T lymphocytes
Objective: To investigate the kinetics of insulin-like growth factor-1 receptor (IGF-1R) expression in PHA-stimulated T lymphocytes. Methods: IGF-1R protein and mRNA were detected by flow cytometry and RT-PCR respectively, between 0 and 48 h after cell activation. Results: Few minutes after T lympho...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_03010163_v59_n6_p276_Segretin |
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todo:paper_03010163_v59_n6_p276_Segretin2023-10-03T15:18:16Z Insulin-like growth factor-1 receptor regulation in activated human T lymphocytes Segretin, M.E. Galeano, A. Roldán, A. Schillaci, R. Down-regulation Insulin-like growth factor-1 receptor T lymphocyte Up-regulation messenger RNA phytohemagglutinin somatomedin C receptor article cell membrane cell surface cell transport flow cytometry human human cell kinetics lymphocyte proliferation priority journal protein expression receptor upregulation reverse transcription polymerase chain reaction T lymphocyte T lymphocyte activation Down-Regulation Flow Cytometry Humans Kinetics Lymphocyte Activation Receptor, IGF Type 1 Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger T-Lymphocytes Up-Regulation Objective: To investigate the kinetics of insulin-like growth factor-1 receptor (IGF-1R) expression in PHA-stimulated T lymphocytes. Methods: IGF-1R protein and mRNA were detected by flow cytometry and RT-PCR respectively, between 0 and 48 h after cell activation. Results: Few minutes after T lymphocytes were activated, internalization of the IGF-1R from the cell membrane was observed, achieving the lower level between 1 and 6 h and was accompanied by a reduction in its mRNA. This was followed by re-expression of IGF-1R on the cell surface and an increase in IGF-1R mRNA levels in the cytoplasm, reaching levels higher than those recorded initially after 48 h activation. Conclusion: This down- and up-regulation suggests that restoration of IGF-1R would be the result of receptor recycling and de novo synthesis and highlights its importance for T lymphocyte proliferation. Copyright © 2003 S. Karger AG, Basel. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03010163_v59_n6_p276_Segretin |
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Universidad de Buenos Aires |
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I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Down-regulation Insulin-like growth factor-1 receptor T lymphocyte Up-regulation messenger RNA phytohemagglutinin somatomedin C receptor article cell membrane cell surface cell transport flow cytometry human human cell kinetics lymphocyte proliferation priority journal protein expression receptor upregulation reverse transcription polymerase chain reaction T lymphocyte T lymphocyte activation Down-Regulation Flow Cytometry Humans Kinetics Lymphocyte Activation Receptor, IGF Type 1 Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger T-Lymphocytes Up-Regulation |
| spellingShingle |
Down-regulation Insulin-like growth factor-1 receptor T lymphocyte Up-regulation messenger RNA phytohemagglutinin somatomedin C receptor article cell membrane cell surface cell transport flow cytometry human human cell kinetics lymphocyte proliferation priority journal protein expression receptor upregulation reverse transcription polymerase chain reaction T lymphocyte T lymphocyte activation Down-Regulation Flow Cytometry Humans Kinetics Lymphocyte Activation Receptor, IGF Type 1 Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger T-Lymphocytes Up-Regulation Segretin, M.E. Galeano, A. Roldán, A. Schillaci, R. Insulin-like growth factor-1 receptor regulation in activated human T lymphocytes |
| topic_facet |
Down-regulation Insulin-like growth factor-1 receptor T lymphocyte Up-regulation messenger RNA phytohemagglutinin somatomedin C receptor article cell membrane cell surface cell transport flow cytometry human human cell kinetics lymphocyte proliferation priority journal protein expression receptor upregulation reverse transcription polymerase chain reaction T lymphocyte T lymphocyte activation Down-Regulation Flow Cytometry Humans Kinetics Lymphocyte Activation Receptor, IGF Type 1 Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger T-Lymphocytes Up-Regulation |
| description |
Objective: To investigate the kinetics of insulin-like growth factor-1 receptor (IGF-1R) expression in PHA-stimulated T lymphocytes. Methods: IGF-1R protein and mRNA were detected by flow cytometry and RT-PCR respectively, between 0 and 48 h after cell activation. Results: Few minutes after T lymphocytes were activated, internalization of the IGF-1R from the cell membrane was observed, achieving the lower level between 1 and 6 h and was accompanied by a reduction in its mRNA. This was followed by re-expression of IGF-1R on the cell surface and an increase in IGF-1R mRNA levels in the cytoplasm, reaching levels higher than those recorded initially after 48 h activation. Conclusion: This down- and up-regulation suggests that restoration of IGF-1R would be the result of receptor recycling and de novo synthesis and highlights its importance for T lymphocyte proliferation. Copyright © 2003 S. Karger AG, Basel. |
| format |
JOUR |
| author |
Segretin, M.E. Galeano, A. Roldán, A. Schillaci, R. |
| author_facet |
Segretin, M.E. Galeano, A. Roldán, A. Schillaci, R. |
| author_sort |
Segretin, M.E. |
| title |
Insulin-like growth factor-1 receptor regulation in activated human T lymphocytes |
| title_short |
Insulin-like growth factor-1 receptor regulation in activated human T lymphocytes |
| title_full |
Insulin-like growth factor-1 receptor regulation in activated human T lymphocytes |
| title_fullStr |
Insulin-like growth factor-1 receptor regulation in activated human T lymphocytes |
| title_full_unstemmed |
Insulin-like growth factor-1 receptor regulation in activated human T lymphocytes |
| title_sort |
insulin-like growth factor-1 receptor regulation in activated human t lymphocytes |
| url |
http://hdl.handle.net/20.500.12110/paper_03010163_v59_n6_p276_Segretin |
| work_keys_str_mv |
AT segretinme insulinlikegrowthfactor1receptorregulationinactivatedhumantlymphocytes AT galeanoa insulinlikegrowthfactor1receptorregulationinactivatedhumantlymphocytes AT roldana insulinlikegrowthfactor1receptorregulationinactivatedhumantlymphocytes AT schillacir insulinlikegrowthfactor1receptorregulationinactivatedhumantlymphocytes |
| _version_ |
1807319997871030272 |