Surface proteins in different isolates of Trypanosoma cruzi epimastigotes

Epimastigotes from several Trypanosoma cruzi stocks were labeled by iodination with Chloramine T and their proteins detected by gel electrophoresis and autoradiography. The labeled proteins from the parasite surface were detected after immunoprecipitation with antisera against fixed trypanosomes or...

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Detalles Bibliográficos
Autores principales: Gonzalez, N.S., Sanchez, D.O., Frasch, A.C.C., Algranati, I.D.
Formato: JOUR
Materias:
membrane protein
parasite antigen
animal
article
immunology
molecular weight
Trypanosoma cruzi
Animal
Antigens, Protozoan
Membrane Proteins
Molecular Weight
Support, Non-U.S. Gov't
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_03008177_v63_n2_p157_Gonzalez
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_03008177_v63_n2_p157_Gonzalez
record_format dspace
spelling todo:paper_03008177_v63_n2_p157_Gonzalez2023-10-03T15:17:52Z Surface proteins in different isolates of Trypanosoma cruzi epimastigotes Gonzalez, N.S. Sanchez, D.O. Frasch, A.C.C. Algranati, I.D. membrane protein parasite antigen animal article immunology molecular weight Trypanosoma cruzi Animal Antigens, Protozoan Membrane Proteins Molecular Weight Support, Non-U.S. Gov't Trypanosoma cruzi Epimastigotes from several Trypanosoma cruzi stocks were labeled by iodination with Chloramine T and their proteins detected by gel electrophoresis and autoradiography. The labeled proteins from the parasite surface were detected after immunoprecipitation with antisera against fixed trypanosomes or from infected rabbits. These antisera were able to recognize one or more proteins in all T. cruzi isolates analyzed, but the individual patterns differed from each other. Variations in the surface protein patterns were also observed in two Tulahuen stocks kept during several years under different conditions. Growth medium as well as the stage of growth at which the parasites were collected had also an effect upon the relative amount of the observed labeled proteins. © 1984 Martinus Nijhoff Publishers. Fil:Gonzalez, N.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Sanchez, D.O. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Algranati, I.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03008177_v63_n2_p157_Gonzalez
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic membrane protein
parasite antigen
animal
article
immunology
molecular weight
Trypanosoma cruzi
Animal
Antigens, Protozoan
Membrane Proteins
Molecular Weight
Support, Non-U.S. Gov't
Trypanosoma cruzi
spellingShingle membrane protein
parasite antigen
animal
article
immunology
molecular weight
Trypanosoma cruzi
Animal
Antigens, Protozoan
Membrane Proteins
Molecular Weight
Support, Non-U.S. Gov't
Trypanosoma cruzi
Gonzalez, N.S.
Sanchez, D.O.
Frasch, A.C.C.
Algranati, I.D.
Surface proteins in different isolates of Trypanosoma cruzi epimastigotes
topic_facet membrane protein
parasite antigen
animal
article
immunology
molecular weight
Trypanosoma cruzi
Animal
Antigens, Protozoan
Membrane Proteins
Molecular Weight
Support, Non-U.S. Gov't
Trypanosoma cruzi
description Epimastigotes from several Trypanosoma cruzi stocks were labeled by iodination with Chloramine T and their proteins detected by gel electrophoresis and autoradiography. The labeled proteins from the parasite surface were detected after immunoprecipitation with antisera against fixed trypanosomes or from infected rabbits. These antisera were able to recognize one or more proteins in all T. cruzi isolates analyzed, but the individual patterns differed from each other. Variations in the surface protein patterns were also observed in two Tulahuen stocks kept during several years under different conditions. Growth medium as well as the stage of growth at which the parasites were collected had also an effect upon the relative amount of the observed labeled proteins. © 1984 Martinus Nijhoff Publishers.
format JOUR
author Gonzalez, N.S.
Sanchez, D.O.
Frasch, A.C.C.
Algranati, I.D.
author_facet Gonzalez, N.S.
Sanchez, D.O.
Frasch, A.C.C.
Algranati, I.D.
author_sort Gonzalez, N.S.
title Surface proteins in different isolates of Trypanosoma cruzi epimastigotes
title_short Surface proteins in different isolates of Trypanosoma cruzi epimastigotes
title_full Surface proteins in different isolates of Trypanosoma cruzi epimastigotes
title_fullStr Surface proteins in different isolates of Trypanosoma cruzi epimastigotes
title_full_unstemmed Surface proteins in different isolates of Trypanosoma cruzi epimastigotes
title_sort surface proteins in different isolates of trypanosoma cruzi epimastigotes
url http://hdl.handle.net/20.500.12110/paper_03008177_v63_n2_p157_Gonzalez
work_keys_str_mv AT gonzalezns surfaceproteinsindifferentisolatesoftrypanosomacruziepimastigotes
AT sanchezdo surfaceproteinsindifferentisolatesoftrypanosomacruziepimastigotes
AT fraschacc surfaceproteinsindifferentisolatesoftrypanosomacruziepimastigotes
AT algranatiid surfaceproteinsindifferentisolatesoftrypanosomacruziepimastigotes
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