Protein kinase from Mucor rouxii - Unshielding of new cyclic AMP binding sites upon dissociation of the ternary complex holoenzyme-cyclic AMP
Cyclic AMP binding to Mucor rouxii protein kinase holoenzyme and free regulatory subunit was measured by the classical membrane filtration technique and by equilibrium dialysis. The results obtained demonstrate that the filtration method can be used without loss of any cyclic AMP binding site. Both...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_03008177_v52_n1_p13_Moreno |
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todo:paper_03008177_v52_n1_p13_Moreno2023-10-03T15:17:51Z Protein kinase from Mucor rouxii - Unshielding of new cyclic AMP binding sites upon dissociation of the ternary complex holoenzyme-cyclic AMP Moreno, S. Pastori, R. Passeron, S. cyclic AMP histone protein kinase article binding site density gradient centrifugation dialysis enzymology filtration macromolecule metabolism methodology Mucor Binding Sites Centrifugation, Density Gradient Cyclic AMP Dialysis Filtration Histones Macromolecular Systems Mucor Protein Kinases Support, Non-U.S. Gov't Cyclic AMP binding to Mucor rouxii protein kinase holoenzyme and free regulatory subunit was measured by the classical membrane filtration technique and by equilibrium dialysis. The results obtained demonstrate that the filtration method can be used without loss of any cyclic AMP binding site. Both methods unambiguously demonstrate that the number of molecules of cyclic AMP bound to the holoenzyme are half of those bound to the regulatory subunit. This result suggests that unshielding of new cyclic AMP binding sites occurs upon dissociation of the ternary complex holoenzyme-cyclic AMP. © 1983 Martinus Nijhoff Publishers. Fil:Moreno, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pastori, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03008177_v52_n1_p13_Moreno |
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Universidad de Buenos Aires |
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I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
cyclic AMP histone protein kinase article binding site density gradient centrifugation dialysis enzymology filtration macromolecule metabolism methodology Mucor Binding Sites Centrifugation, Density Gradient Cyclic AMP Dialysis Filtration Histones Macromolecular Systems Mucor Protein Kinases Support, Non-U.S. Gov't |
| spellingShingle |
cyclic AMP histone protein kinase article binding site density gradient centrifugation dialysis enzymology filtration macromolecule metabolism methodology Mucor Binding Sites Centrifugation, Density Gradient Cyclic AMP Dialysis Filtration Histones Macromolecular Systems Mucor Protein Kinases Support, Non-U.S. Gov't Moreno, S. Pastori, R. Passeron, S. Protein kinase from Mucor rouxii - Unshielding of new cyclic AMP binding sites upon dissociation of the ternary complex holoenzyme-cyclic AMP |
| topic_facet |
cyclic AMP histone protein kinase article binding site density gradient centrifugation dialysis enzymology filtration macromolecule metabolism methodology Mucor Binding Sites Centrifugation, Density Gradient Cyclic AMP Dialysis Filtration Histones Macromolecular Systems Mucor Protein Kinases Support, Non-U.S. Gov't |
| description |
Cyclic AMP binding to Mucor rouxii protein kinase holoenzyme and free regulatory subunit was measured by the classical membrane filtration technique and by equilibrium dialysis. The results obtained demonstrate that the filtration method can be used without loss of any cyclic AMP binding site. Both methods unambiguously demonstrate that the number of molecules of cyclic AMP bound to the holoenzyme are half of those bound to the regulatory subunit. This result suggests that unshielding of new cyclic AMP binding sites occurs upon dissociation of the ternary complex holoenzyme-cyclic AMP. © 1983 Martinus Nijhoff Publishers. |
| format |
JOUR |
| author |
Moreno, S. Pastori, R. Passeron, S. |
| author_facet |
Moreno, S. Pastori, R. Passeron, S. |
| author_sort |
Moreno, S. |
| title |
Protein kinase from Mucor rouxii - Unshielding of new cyclic AMP binding sites upon dissociation of the ternary complex holoenzyme-cyclic AMP |
| title_short |
Protein kinase from Mucor rouxii - Unshielding of new cyclic AMP binding sites upon dissociation of the ternary complex holoenzyme-cyclic AMP |
| title_full |
Protein kinase from Mucor rouxii - Unshielding of new cyclic AMP binding sites upon dissociation of the ternary complex holoenzyme-cyclic AMP |
| title_fullStr |
Protein kinase from Mucor rouxii - Unshielding of new cyclic AMP binding sites upon dissociation of the ternary complex holoenzyme-cyclic AMP |
| title_full_unstemmed |
Protein kinase from Mucor rouxii - Unshielding of new cyclic AMP binding sites upon dissociation of the ternary complex holoenzyme-cyclic AMP |
| title_sort |
protein kinase from mucor rouxii - unshielding of new cyclic amp binding sites upon dissociation of the ternary complex holoenzyme-cyclic amp |
| url |
http://hdl.handle.net/20.500.12110/paper_03008177_v52_n1_p13_Moreno |
| work_keys_str_mv |
AT morenos proteinkinasefrommucorrouxiiunshieldingofnewcyclicampbindingsitesupondissociationoftheternarycomplexholoenzymecyclicamp AT pastorir proteinkinasefrommucorrouxiiunshieldingofnewcyclicampbindingsitesupondissociationoftheternarycomplexholoenzymecyclicamp AT passerons proteinkinasefrommucorrouxiiunshieldingofnewcyclicampbindingsitesupondissociationoftheternarycomplexholoenzymecyclicamp |
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1807324240085516288 |