Inhibition of cyclic 3′5′ monophosphate synthesis in rat testis by L-triiodothyronine

Cytosolic adenylate cyclase activity from rat seminiferous tubules is inhibited by L-triiodothyronine (L-T3). In a typical dose-response curve, using Mn-ATP as substrate, no effect is observed at 10-10 M L-T3; about 15 to 25% inhibition is found in the range between 10-9 and 10-6 M L-T3 and finally...

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Detalles Bibliográficos
Autores principales: Flawia, M.M., Kornblihtt, A.R., de Mendoza, D., Torres, H.N.
Formato: JOUR
Materias:
adenylate cyclase
cyclic amp
liothyronine
animal experiment
drug response
endocrine system
enzyme inhibition
hormone sensitivity
in vitro study
male genital system
preliminary communication
rat
seminiferous tubule
testis
Adenylate Cyclase
Animal
Cyclic AMP
Cytosol
Dose-Response Relationship, Drug
Hydrogen-Ion Concentration
Kinetics
Magnesium
Male
Manganese
Rats
Subcellular Fractions
Support, Non-U.S. Gov't
Testis
Triiodothyronine
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_03008177_v34_n3_p185_Flawia
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:Cytosolic adenylate cyclase activity from rat seminiferous tubules is inhibited by L-triiodothyronine (L-T3). In a typical dose-response curve, using Mn-ATP as substrate, no effect is observed at 10-10 M L-T3; about 15 to 25% inhibition is found in the range between 10-9 and 10-6 M L-T3 and finally a sharp enzyme inhibition is evident at increasing hormone concentrations from 10-6 to 10-4 M. Incubation of decapsulated testes with L-T3 leads to a decrease of intracellular cyclic AMP levels. Dose-response relationships for such effect are similar to those found for adenylate cyclase activity. In this case a clear response is observed at 10-8 M L-T3. © 1981 Martinus Nijhoff Dr. W. Juk Publishers B.V.

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