Inhibition of cyclic 3′5′ monophosphate synthesis in rat testis by L-triiodothyronine

Cytosolic adenylate cyclase activity from rat seminiferous tubules is inhibited by L-triiodothyronine (L-T3). In a typical dose-response curve, using Mn-ATP as substrate, no effect is observed at 10-10 M L-T3; about 15 to 25% inhibition is found in the range between 10-9 and 10-6 M L-T3 and finally...

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Autores principales: Flawia, M.M., Kornblihtt, A.R., de Mendoza, D., Torres, H.N.
Formato: JOUR
Materias:
adenylate cyclase
cyclic amp
liothyronine
animal experiment
drug response
endocrine system
enzyme inhibition
hormone sensitivity
in vitro study
male genital system
preliminary communication
rat
seminiferous tubule
testis
Adenylate Cyclase
Animal
Cyclic AMP
Cytosol
Dose-Response Relationship, Drug
Hydrogen-Ion Concentration
Kinetics
Magnesium
Male
Manganese
Rats
Subcellular Fractions
Support, Non-U.S. Gov't
Testis
Triiodothyronine
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_03008177_v34_n3_p185_Flawia
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_03008177_v34_n3_p185_Flawia
record_format dspace
spelling todo:paper_03008177_v34_n3_p185_Flawia2023-10-03T15:17:47Z Inhibition of cyclic 3′5′ monophosphate synthesis in rat testis by L-triiodothyronine Flawia, M.M. Kornblihtt, A.R. de Mendoza, D. Torres, H.N. adenylate cyclase cyclic amp liothyronine animal experiment drug response endocrine system enzyme inhibition hormone sensitivity in vitro study male genital system preliminary communication rat seminiferous tubule testis Adenylate Cyclase Animal Cyclic AMP Cytosol Dose-Response Relationship, Drug Hydrogen-Ion Concentration Kinetics Magnesium Male Manganese Rats Subcellular Fractions Support, Non-U.S. Gov't Testis Triiodothyronine Cytosolic adenylate cyclase activity from rat seminiferous tubules is inhibited by L-triiodothyronine (L-T3). In a typical dose-response curve, using Mn-ATP as substrate, no effect is observed at 10-10 M L-T3; about 15 to 25% inhibition is found in the range between 10-9 and 10-6 M L-T3 and finally a sharp enzyme inhibition is evident at increasing hormone concentrations from 10-6 to 10-4 M. Incubation of decapsulated testes with L-T3 leads to a decrease of intracellular cyclic AMP levels. Dose-response relationships for such effect are similar to those found for adenylate cyclase activity. In this case a clear response is observed at 10-8 M L-T3. © 1981 Martinus Nijhoff Dr. W. Juk Publishers B.V. Fil:Flawia, M.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Kornblihtt, A.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Torres, H.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03008177_v34_n3_p185_Flawia
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic adenylate cyclase
cyclic amp
liothyronine
animal experiment
drug response
endocrine system
enzyme inhibition
hormone sensitivity
in vitro study
male genital system
preliminary communication
rat
seminiferous tubule
testis
Adenylate Cyclase
Animal
Cyclic AMP
Cytosol
Dose-Response Relationship, Drug
Hydrogen-Ion Concentration
Kinetics
Magnesium
Male
Manganese
Rats
Subcellular Fractions
Support, Non-U.S. Gov't
Testis
Triiodothyronine
spellingShingle adenylate cyclase
cyclic amp
liothyronine
animal experiment
drug response
endocrine system
enzyme inhibition
hormone sensitivity
in vitro study
male genital system
preliminary communication
rat
seminiferous tubule
testis
Adenylate Cyclase
Animal
Cyclic AMP
Cytosol
Dose-Response Relationship, Drug
Hydrogen-Ion Concentration
Kinetics
Magnesium
Male
Manganese
Rats
Subcellular Fractions
Support, Non-U.S. Gov't
Testis
Triiodothyronine
Flawia, M.M.
Kornblihtt, A.R.
de Mendoza, D.
Torres, H.N.
Inhibition of cyclic 3′5′ monophosphate synthesis in rat testis by L-triiodothyronine
topic_facet adenylate cyclase
cyclic amp
liothyronine
animal experiment
drug response
endocrine system
enzyme inhibition
hormone sensitivity
in vitro study
male genital system
preliminary communication
rat
seminiferous tubule
testis
Adenylate Cyclase
Animal
Cyclic AMP
Cytosol
Dose-Response Relationship, Drug
Hydrogen-Ion Concentration
Kinetics
Magnesium
Male
Manganese
Rats
Subcellular Fractions
Support, Non-U.S. Gov't
Testis
Triiodothyronine
description Cytosolic adenylate cyclase activity from rat seminiferous tubules is inhibited by L-triiodothyronine (L-T3). In a typical dose-response curve, using Mn-ATP as substrate, no effect is observed at 10-10 M L-T3; about 15 to 25% inhibition is found in the range between 10-9 and 10-6 M L-T3 and finally a sharp enzyme inhibition is evident at increasing hormone concentrations from 10-6 to 10-4 M. Incubation of decapsulated testes with L-T3 leads to a decrease of intracellular cyclic AMP levels. Dose-response relationships for such effect are similar to those found for adenylate cyclase activity. In this case a clear response is observed at 10-8 M L-T3. © 1981 Martinus Nijhoff Dr. W. Juk Publishers B.V.
format JOUR
author Flawia, M.M.
Kornblihtt, A.R.
de Mendoza, D.
Torres, H.N.
author_facet Flawia, M.M.
Kornblihtt, A.R.
de Mendoza, D.
Torres, H.N.
author_sort Flawia, M.M.
title Inhibition of cyclic 3′5′ monophosphate synthesis in rat testis by L-triiodothyronine
title_short Inhibition of cyclic 3′5′ monophosphate synthesis in rat testis by L-triiodothyronine
title_full Inhibition of cyclic 3′5′ monophosphate synthesis in rat testis by L-triiodothyronine
title_fullStr Inhibition of cyclic 3′5′ monophosphate synthesis in rat testis by L-triiodothyronine
title_full_unstemmed Inhibition of cyclic 3′5′ monophosphate synthesis in rat testis by L-triiodothyronine
title_sort inhibition of cyclic 3′5′ monophosphate synthesis in rat testis by l-triiodothyronine
url http://hdl.handle.net/20.500.12110/paper_03008177_v34_n3_p185_Flawia
work_keys_str_mv AT flawiamm inhibitionofcyclic35monophosphatesynthesisinrattestisbyltriiodothyronine
AT kornblihttar inhibitionofcyclic35monophosphatesynthesisinrattestisbyltriiodothyronine
AT demendozad inhibitionofcyclic35monophosphatesynthesisinrattestisbyltriiodothyronine
AT torreshn inhibitionofcyclic35monophosphatesynthesisinrattestisbyltriiodothyronine
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