Polyamines and protein synthesis: Studies in various polyamine-requiring mutants of Escherichia coli

Different Escherichia coli mutants auxotrophic for polyamines were studied in order to investigate the relationships among polypeptide synthesis in cell-free systems, ribosomal distribution profiles and endogenous polyamine pools. The in vitro protein synthetic activity and the polyribosomal content...

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Detalles Bibliográficos
Autores principales: Goldemberg, S.H., Algranati, I.D.
Formato: JOUR
Materias:
cadaverine
putrescine
spermidine
auxotrophic mutant
escherichia coli
in vitro study
microorganism
protein synthesis
theoretical study
Bacterial Proteins
Cell Division
Escherichia coli
Mutation
Putrescine
Ribosomes
Species Specificity
Time Factors
Translation, Genetic
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_03008177_v16_n2-3_p71_Goldemberg
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_03008177_v16_n2-3_p71_Goldemberg
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spelling todo:paper_03008177_v16_n2-3_p71_Goldemberg2023-10-03T15:17:45Z Polyamines and protein synthesis: Studies in various polyamine-requiring mutants of Escherichia coli Goldemberg, S.H. Algranati, I.D. cadaverine putrescine spermidine auxotrophic mutant escherichia coli in vitro study microorganism protein synthesis theoretical study Bacterial Proteins Cell Division Escherichia coli Mutation Putrescine Ribosomes Species Specificity Time Factors Translation, Genetic Different Escherichia coli mutants auxotrophic for polyamines were studied in order to investigate the relationships among polypeptide synthesis in cell-free systems, ribosomal distribution profiles and endogenous polyamine pools. The in vitro protein synthetic activity and the polyribosomal content were reduced in extracts from putrescine-starved cells of the double mutants MA 255 and MA 261, but not in the arginine-conditional auxotroph DK 6. Putrescine addition to the cultures of all these strains previously starved for polyamines, provoked a shift towards monomers in the equilibrium involving ribosomal particles. Concomitant changes in the intracellular levels of polyamines were observed: putrescine and spermidine increased markedly, and cadaverine disappeared. © 1977 Dr. W. Junk b.v. Publishers. Fil:Goldemberg, S.H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Algranati, I.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03008177_v16_n2-3_p71_Goldemberg
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic cadaverine
putrescine
spermidine
auxotrophic mutant
escherichia coli
in vitro study
microorganism
protein synthesis
theoretical study
Bacterial Proteins
Cell Division
Escherichia coli
Mutation
Putrescine
Ribosomes
Species Specificity
Time Factors
Translation, Genetic
spellingShingle cadaverine
putrescine
spermidine
auxotrophic mutant
escherichia coli
in vitro study
microorganism
protein synthesis
theoretical study
Bacterial Proteins
Cell Division
Escherichia coli
Mutation
Putrescine
Ribosomes
Species Specificity
Time Factors
Translation, Genetic
Goldemberg, S.H.
Algranati, I.D.
Polyamines and protein synthesis: Studies in various polyamine-requiring mutants of Escherichia coli
topic_facet cadaverine
putrescine
spermidine
auxotrophic mutant
escherichia coli
in vitro study
microorganism
protein synthesis
theoretical study
Bacterial Proteins
Cell Division
Escherichia coli
Mutation
Putrescine
Ribosomes
Species Specificity
Time Factors
Translation, Genetic
description Different Escherichia coli mutants auxotrophic for polyamines were studied in order to investigate the relationships among polypeptide synthesis in cell-free systems, ribosomal distribution profiles and endogenous polyamine pools. The in vitro protein synthetic activity and the polyribosomal content were reduced in extracts from putrescine-starved cells of the double mutants MA 255 and MA 261, but not in the arginine-conditional auxotroph DK 6. Putrescine addition to the cultures of all these strains previously starved for polyamines, provoked a shift towards monomers in the equilibrium involving ribosomal particles. Concomitant changes in the intracellular levels of polyamines were observed: putrescine and spermidine increased markedly, and cadaverine disappeared. © 1977 Dr. W. Junk b.v. Publishers.
format JOUR
author Goldemberg, S.H.
Algranati, I.D.
author_facet Goldemberg, S.H.
Algranati, I.D.
author_sort Goldemberg, S.H.
title Polyamines and protein synthesis: Studies in various polyamine-requiring mutants of Escherichia coli
title_short Polyamines and protein synthesis: Studies in various polyamine-requiring mutants of Escherichia coli
title_full Polyamines and protein synthesis: Studies in various polyamine-requiring mutants of Escherichia coli
title_fullStr Polyamines and protein synthesis: Studies in various polyamine-requiring mutants of Escherichia coli
title_full_unstemmed Polyamines and protein synthesis: Studies in various polyamine-requiring mutants of Escherichia coli
title_sort polyamines and protein synthesis: studies in various polyamine-requiring mutants of escherichia coli
url http://hdl.handle.net/20.500.12110/paper_03008177_v16_n2-3_p71_Goldemberg
work_keys_str_mv AT goldembergsh polyaminesandproteinsynthesisstudiesinvariouspolyaminerequiringmutantsofescherichiacoli
AT algranatiid polyaminesandproteinsynthesisstudiesinvariouspolyaminerequiringmutantsofescherichiacoli
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