Inhibition of cholinesterase activity by azinphos-methyl in two freshwater invertebrates: Biomphalaria glabrata and Lumbriculus variegatus
In this study, some biochemical features and the extent of inhibition induced by the organophosphorous pesticide azinphos-methyl on the cholinesterase (ChE) activity present in whole soft tissue of two freshwater invertebrate species, the gastropod Biomphalaria glabrata and the oligochaete Lumbricul...
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todo:paper_0300483X_v222_n3_p185_Kristoff2023-10-03T15:17:35Z Inhibition of cholinesterase activity by azinphos-methyl in two freshwater invertebrates: Biomphalaria glabrata and Lumbriculus variegatus Kristoff, G. Guerrero, N.V. de D'Angelo, A.M.P. Cochón, A.C. Azinphos-methyl Biomphalaria glabrata Cholinesterase Lumbriculus variegatus acetylthiocholine azinphos methyl butyrylthiocholine cholinesterase insecticide organophosphate pesticide outer membrane protein C physostigmine water animal experiment annelid worm article Biomphalaria glabrata cellular distribution centrifugation concentration (parameters) controlled study enzyme activity enzyme inhibition enzyme specificity freshwater environment gastropod invertebrate lumbriculus variegatus mortality nonhuman priority journal soft tissue supernatant Animals Azinphosmethyl Biological Markers Cholinesterase Inhibitors Cholinesterases Fresh Water Insecticides Oligochaeta Snails Species Specificity Animalia Annelida Biomphalaria glabrata Gastropoda Invertebrata Lumbriculus variegatus Oligochaeta (Metazoa) In this study, some biochemical features and the extent of inhibition induced by the organophosphorous pesticide azinphos-methyl on the cholinesterase (ChE) activity present in whole soft tissue of two freshwater invertebrate species, the gastropod Biomphalaria glabrata and the oligochaete Lumbriculus variegatus were investigated. Both invertebrate organisms presented marked differences in ChE activity, type of enzymes and subcellular location. Acetylthiocholine was the substrate preferred by B. glabrata ChE. The enzyme activity was located preferentially in the supernatant of 11,000 × g centrifugation and was inhibited by increasing concentrations of substrate but not by iso-OMPA. Results showed that there were progressive inhibitions of the enzyme activity, with values 21%, 59%, 72%, 76%, and 82% lower than the control at levels of 1, 10, 50, 100 and 1000 μM of eserine, respectively. In contrast, L. variegatus ChE activity was distributed both in the supernatant and pellet fractions, with values approximately 6 and 20 times higher than B. glabrata, respectively. Studies with butyrylthiocholine and iso-OMPA suggested that about 72% of the activity corresponded to butyrylcholinesterase. A strong enzyme inhibition (88-94%) was found at low eserine concentrations (1-10 μM). ChE activity from L. variegatus and B. glabrata was inhibited by in vivo exposure to azinphos-methyl suggesting that both species can form the oxon derivative of this pesticide. However, both invertebrate species showed a very different susceptibility to the insecticide. The NOEC and EIC50 values were 500 and 1000 times lower for L. variegatus than for B. glabrata, reflecting that the oligochaetes were much more sensitive organisms. A different pattern was also observed for the recovery of the enzymatic activity when the organisms were transferred to clean water. The recuperation process was faster for the oligochaetes than for the gastropods. Mortality was not observed in either of the experimental conditions assayed, not even at concentrations that induced 90% of ChE inhibition. The differences in substrate specificity, sensitivity to inhibitors, and subcellular location between the ChEs of B. glabrata and L. variegatus could be the main factors contributing to the differential susceptibility to azinphos-methyl ChE inhibition found in the present study. © 2006 Elsevier Ireland Ltd. All rights reserved. Fil:Kristoff, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cochón, A.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0300483X_v222_n3_p185_Kristoff |
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Universidad de Buenos Aires |
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I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Azinphos-methyl Biomphalaria glabrata Cholinesterase Lumbriculus variegatus acetylthiocholine azinphos methyl butyrylthiocholine cholinesterase insecticide organophosphate pesticide outer membrane protein C physostigmine water animal experiment annelid worm article Biomphalaria glabrata cellular distribution centrifugation concentration (parameters) controlled study enzyme activity enzyme inhibition enzyme specificity freshwater environment gastropod invertebrate lumbriculus variegatus mortality nonhuman priority journal soft tissue supernatant Animals Azinphosmethyl Biological Markers Cholinesterase Inhibitors Cholinesterases Fresh Water Insecticides Oligochaeta Snails Species Specificity Animalia Annelida Biomphalaria glabrata Gastropoda Invertebrata Lumbriculus variegatus Oligochaeta (Metazoa) |
| spellingShingle |
Azinphos-methyl Biomphalaria glabrata Cholinesterase Lumbriculus variegatus acetylthiocholine azinphos methyl butyrylthiocholine cholinesterase insecticide organophosphate pesticide outer membrane protein C physostigmine water animal experiment annelid worm article Biomphalaria glabrata cellular distribution centrifugation concentration (parameters) controlled study enzyme activity enzyme inhibition enzyme specificity freshwater environment gastropod invertebrate lumbriculus variegatus mortality nonhuman priority journal soft tissue supernatant Animals Azinphosmethyl Biological Markers Cholinesterase Inhibitors Cholinesterases Fresh Water Insecticides Oligochaeta Snails Species Specificity Animalia Annelida Biomphalaria glabrata Gastropoda Invertebrata Lumbriculus variegatus Oligochaeta (Metazoa) Kristoff, G. Guerrero, N.V. de D'Angelo, A.M.P. Cochón, A.C. Inhibition of cholinesterase activity by azinphos-methyl in two freshwater invertebrates: Biomphalaria glabrata and Lumbriculus variegatus |
| topic_facet |
Azinphos-methyl Biomphalaria glabrata Cholinesterase Lumbriculus variegatus acetylthiocholine azinphos methyl butyrylthiocholine cholinesterase insecticide organophosphate pesticide outer membrane protein C physostigmine water animal experiment annelid worm article Biomphalaria glabrata cellular distribution centrifugation concentration (parameters) controlled study enzyme activity enzyme inhibition enzyme specificity freshwater environment gastropod invertebrate lumbriculus variegatus mortality nonhuman priority journal soft tissue supernatant Animals Azinphosmethyl Biological Markers Cholinesterase Inhibitors Cholinesterases Fresh Water Insecticides Oligochaeta Snails Species Specificity Animalia Annelida Biomphalaria glabrata Gastropoda Invertebrata Lumbriculus variegatus Oligochaeta (Metazoa) |
| description |
In this study, some biochemical features and the extent of inhibition induced by the organophosphorous pesticide azinphos-methyl on the cholinesterase (ChE) activity present in whole soft tissue of two freshwater invertebrate species, the gastropod Biomphalaria glabrata and the oligochaete Lumbriculus variegatus were investigated. Both invertebrate organisms presented marked differences in ChE activity, type of enzymes and subcellular location. Acetylthiocholine was the substrate preferred by B. glabrata ChE. The enzyme activity was located preferentially in the supernatant of 11,000 × g centrifugation and was inhibited by increasing concentrations of substrate but not by iso-OMPA. Results showed that there were progressive inhibitions of the enzyme activity, with values 21%, 59%, 72%, 76%, and 82% lower than the control at levels of 1, 10, 50, 100 and 1000 μM of eserine, respectively. In contrast, L. variegatus ChE activity was distributed both in the supernatant and pellet fractions, with values approximately 6 and 20 times higher than B. glabrata, respectively. Studies with butyrylthiocholine and iso-OMPA suggested that about 72% of the activity corresponded to butyrylcholinesterase. A strong enzyme inhibition (88-94%) was found at low eserine concentrations (1-10 μM). ChE activity from L. variegatus and B. glabrata was inhibited by in vivo exposure to azinphos-methyl suggesting that both species can form the oxon derivative of this pesticide. However, both invertebrate species showed a very different susceptibility to the insecticide. The NOEC and EIC50 values were 500 and 1000 times lower for L. variegatus than for B. glabrata, reflecting that the oligochaetes were much more sensitive organisms. A different pattern was also observed for the recovery of the enzymatic activity when the organisms were transferred to clean water. The recuperation process was faster for the oligochaetes than for the gastropods. Mortality was not observed in either of the experimental conditions assayed, not even at concentrations that induced 90% of ChE inhibition. The differences in substrate specificity, sensitivity to inhibitors, and subcellular location between the ChEs of B. glabrata and L. variegatus could be the main factors contributing to the differential susceptibility to azinphos-methyl ChE inhibition found in the present study. © 2006 Elsevier Ireland Ltd. All rights reserved. |
| format |
JOUR |
| author |
Kristoff, G. Guerrero, N.V. de D'Angelo, A.M.P. Cochón, A.C. |
| author_facet |
Kristoff, G. Guerrero, N.V. de D'Angelo, A.M.P. Cochón, A.C. |
| author_sort |
Kristoff, G. |
| title |
Inhibition of cholinesterase activity by azinphos-methyl in two freshwater invertebrates: Biomphalaria glabrata and Lumbriculus variegatus |
| title_short |
Inhibition of cholinesterase activity by azinphos-methyl in two freshwater invertebrates: Biomphalaria glabrata and Lumbriculus variegatus |
| title_full |
Inhibition of cholinesterase activity by azinphos-methyl in two freshwater invertebrates: Biomphalaria glabrata and Lumbriculus variegatus |
| title_fullStr |
Inhibition of cholinesterase activity by azinphos-methyl in two freshwater invertebrates: Biomphalaria glabrata and Lumbriculus variegatus |
| title_full_unstemmed |
Inhibition of cholinesterase activity by azinphos-methyl in two freshwater invertebrates: Biomphalaria glabrata and Lumbriculus variegatus |
| title_sort |
inhibition of cholinesterase activity by azinphos-methyl in two freshwater invertebrates: biomphalaria glabrata and lumbriculus variegatus |
| url |
http://hdl.handle.net/20.500.12110/paper_0300483X_v222_n3_p185_Kristoff |
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