Hexachlorobenzene-induced alterations of rat hepatic microsomal membrane function
The time and dose-dependent effects of the in vivo administration of hexachlorobenzene (HCB), on hepatic microsomal membrane functions, were studied in female Wistar rats. Administration of HCB (100 mg/100 g b.w.) resulted in time-dependent decreases in the activity of two membrane-bound enzymes: 5&...
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todo:paper_0300483X_v125_n2-3_p83_Randi2023-10-03T15:17:33Z Hexachlorobenzene-induced alterations of rat hepatic microsomal membrane function Randi, A.S. Sancovich, H.A. Ferramola De Sancovich, A.M. Loaiza, A. Krawiec, L. Kleiman De Pisarev, D.L. Epidermal growth factor receptor Hexachlorobenzene Mechanism of action Membrane protein phosphorylation Protein tyrosine kinase epidermal growth factor receptor hexachlorobenzene protein tyrosine kinase animal experiment article dose response drug effect enzyme activation enzyme activity female liver microsome liver toxicity nonhuman priority journal protein phosphorylation rat 5'-Nucleotidase Animals Dose-Response Relationship, Drug Epidermal Growth Factor Female Hexachlorobenzene Intracellular Membranes Microsomes, Liver Na(+)-K(+)-Exchanging ATPase Phosphorylation Protein-Tyrosine Kinases Rats Rats, Wistar Receptor, Epidermal Growth Factor Soil Pollutants The time and dose-dependent effects of the in vivo administration of hexachlorobenzene (HCB), on hepatic microsomal membrane functions, were studied in female Wistar rats. Administration of HCB (100 mg/100 g b.w.) resulted in time-dependent decreases in the activity of two membrane-bound enzymes: 5'nucleotidase and Na+/K+ ATPase. HCB was found to cause a significant rise in protein tyrosine kinase (PTK) activity during the early stages of intoxication (day 2), followed by a significant decrease at 10 days, returning to control levels after 20 days of treatment. A stimulatory effect of HCB on in vitro endogenous microsomal protein phosphorylation was observed from 2 days of intoxication up to 30 days of treatment, with an important stimulation of phosphorylation at 5 days. Administration of HCB (100 mg/100 g b.w.) for 10 days caused a 50% reduction in epidermal growth factor receptor (EGF-R) ligand binding. The effects of known specific inhibitors of protein phosphatases on endogenous protein phosphorylation were studied. HCB affected the labelling of several bands, as well as the 5'nucleotidase and PTK activities, in a dose-dependent manner. In conclusion, this study indicated that the in vivo administration of HCB results in a significant alteration of membrane function. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0300483X_v125_n2-3_p83_Randi |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Epidermal growth factor receptor Hexachlorobenzene Mechanism of action Membrane protein phosphorylation Protein tyrosine kinase epidermal growth factor receptor hexachlorobenzene protein tyrosine kinase animal experiment article dose response drug effect enzyme activation enzyme activity female liver microsome liver toxicity nonhuman priority journal protein phosphorylation rat 5'-Nucleotidase Animals Dose-Response Relationship, Drug Epidermal Growth Factor Female Hexachlorobenzene Intracellular Membranes Microsomes, Liver Na(+)-K(+)-Exchanging ATPase Phosphorylation Protein-Tyrosine Kinases Rats Rats, Wistar Receptor, Epidermal Growth Factor Soil Pollutants |
| spellingShingle |
Epidermal growth factor receptor Hexachlorobenzene Mechanism of action Membrane protein phosphorylation Protein tyrosine kinase epidermal growth factor receptor hexachlorobenzene protein tyrosine kinase animal experiment article dose response drug effect enzyme activation enzyme activity female liver microsome liver toxicity nonhuman priority journal protein phosphorylation rat 5'-Nucleotidase Animals Dose-Response Relationship, Drug Epidermal Growth Factor Female Hexachlorobenzene Intracellular Membranes Microsomes, Liver Na(+)-K(+)-Exchanging ATPase Phosphorylation Protein-Tyrosine Kinases Rats Rats, Wistar Receptor, Epidermal Growth Factor Soil Pollutants Randi, A.S. Sancovich, H.A. Ferramola De Sancovich, A.M. Loaiza, A. Krawiec, L. Kleiman De Pisarev, D.L. Hexachlorobenzene-induced alterations of rat hepatic microsomal membrane function |
| topic_facet |
Epidermal growth factor receptor Hexachlorobenzene Mechanism of action Membrane protein phosphorylation Protein tyrosine kinase epidermal growth factor receptor hexachlorobenzene protein tyrosine kinase animal experiment article dose response drug effect enzyme activation enzyme activity female liver microsome liver toxicity nonhuman priority journal protein phosphorylation rat 5'-Nucleotidase Animals Dose-Response Relationship, Drug Epidermal Growth Factor Female Hexachlorobenzene Intracellular Membranes Microsomes, Liver Na(+)-K(+)-Exchanging ATPase Phosphorylation Protein-Tyrosine Kinases Rats Rats, Wistar Receptor, Epidermal Growth Factor Soil Pollutants |
| description |
The time and dose-dependent effects of the in vivo administration of hexachlorobenzene (HCB), on hepatic microsomal membrane functions, were studied in female Wistar rats. Administration of HCB (100 mg/100 g b.w.) resulted in time-dependent decreases in the activity of two membrane-bound enzymes: 5'nucleotidase and Na+/K+ ATPase. HCB was found to cause a significant rise in protein tyrosine kinase (PTK) activity during the early stages of intoxication (day 2), followed by a significant decrease at 10 days, returning to control levels after 20 days of treatment. A stimulatory effect of HCB on in vitro endogenous microsomal protein phosphorylation was observed from 2 days of intoxication up to 30 days of treatment, with an important stimulation of phosphorylation at 5 days. Administration of HCB (100 mg/100 g b.w.) for 10 days caused a 50% reduction in epidermal growth factor receptor (EGF-R) ligand binding. The effects of known specific inhibitors of protein phosphatases on endogenous protein phosphorylation were studied. HCB affected the labelling of several bands, as well as the 5'nucleotidase and PTK activities, in a dose-dependent manner. In conclusion, this study indicated that the in vivo administration of HCB results in a significant alteration of membrane function. |
| format |
JOUR |
| author |
Randi, A.S. Sancovich, H.A. Ferramola De Sancovich, A.M. Loaiza, A. Krawiec, L. Kleiman De Pisarev, D.L. |
| author_facet |
Randi, A.S. Sancovich, H.A. Ferramola De Sancovich, A.M. Loaiza, A. Krawiec, L. Kleiman De Pisarev, D.L. |
| author_sort |
Randi, A.S. |
| title |
Hexachlorobenzene-induced alterations of rat hepatic microsomal membrane function |
| title_short |
Hexachlorobenzene-induced alterations of rat hepatic microsomal membrane function |
| title_full |
Hexachlorobenzene-induced alterations of rat hepatic microsomal membrane function |
| title_fullStr |
Hexachlorobenzene-induced alterations of rat hepatic microsomal membrane function |
| title_full_unstemmed |
Hexachlorobenzene-induced alterations of rat hepatic microsomal membrane function |
| title_sort |
hexachlorobenzene-induced alterations of rat hepatic microsomal membrane function |
| url |
http://hdl.handle.net/20.500.12110/paper_0300483X_v125_n2-3_p83_Randi |
| work_keys_str_mv |
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| _version_ |
1807317633583808512 |