Histochemical characterization of oocytes in the pink Cuskeel (Genypterus blacodes)

In the present study we histochemically and lectinhistochemically characterized the growing oocytes of the pink cuskeel (Genypterus blacodes). We used histochemical methods for the localization and characterization of glycoconjugates (GCs) and lectin histochemical techniques for the identification o...

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Autores principales: Cohen, S., Petcoff, G., Freijo, R.O., Portiansky, E.L., Barbeito, C.G., Macchi, G.J., Díaz, A.O.
Formato: JOUR
Materias:
Genypterus blacodes
Glycoconjugates
Histochemistry
Lectins
Oocytes
Radiata
Teleostei
animal
cytology
female
fish
oocyte
physiology
Animals
Female
Fishes
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_02890003_v32_n4_p383_Cohen
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
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spelling todo:paper_02890003_v32_n4_p383_Cohen2023-10-03T15:17:16Z Histochemical characterization of oocytes in the pink Cuskeel (Genypterus blacodes) Cohen, S. Petcoff, G. Freijo, R.O. Portiansky, E.L. Barbeito, C.G. Macchi, G.J. Díaz, A.O. Genypterus blacodes Glycoconjugates Histochemistry Lectins Oocytes Genypterus blacodes Radiata Teleostei animal cytology female fish oocyte physiology Animals Female Fishes Oocytes In the present study we histochemically and lectinhistochemically characterized the growing oocytes of the pink cuskeel (Genypterus blacodes). We used histochemical methods for the localization and characterization of glycoconjugates (GCs) and lectin histochemical techniques for the identification of specific sugar residues. We analyzed presence and distribution of GCs in the different structures of the growing follicles (cortical alveoli, globules, yolk granules and zona radiata). During the initial stage of vitellogenesis, the oocytes presented small yolk granules composed of GCs that gradually increased during exogenous vitellogenesis. These GCs contained moderate quantities of α-D-mannose, D-glucose, N-acetylglucosamine and N-acetyl-neuraminic acid. The cortical alveoli contained both neutral and carboxylated GCs, and lectin techniques detected N-acetylgalactosamine, galactose and L-fucose. The zona radiata showed a strong positive reaction to PAS and it reacted weakly with more specific techniques, such as KOH/PAS and PA/Bh/KOH/PAS. This structure showed GCs with oxidizable vicinal diols, O-acyl sugars and sialic acid residues with different substitution types and presented N-acetylgalactosamine and L-fucose specific residues. The oocytes follicular envelope evidenced neutral and acidic non-sulfated GCs and high concentrations of α-D-mannose, D-glucose, galactose and N-acetylgalactosamine. The intergranular cytoplasmic GCs were mainly rich in α-D-mannose, D-glucose, N-acetylgalactosamine, N-acetylglucosamine and N-acetyl-neuraminic acid. These results enhance the comprehension of the structure and functionality of the pink cuskeel ovarian follicles, and provide a useful tool for the study of this tissue in other teleost species. © 2015 Zoological Society of Japan. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_02890003_v32_n4_p383_Cohen
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Genypterus blacodes
Glycoconjugates
Histochemistry
Lectins
Oocytes
Genypterus blacodes
Radiata
Teleostei
animal
cytology
female
fish
oocyte
physiology
Animals
Female
Fishes
Oocytes
spellingShingle Genypterus blacodes
Glycoconjugates
Histochemistry
Lectins
Oocytes
Genypterus blacodes
Radiata
Teleostei
animal
cytology
female
fish
oocyte
physiology
Animals
Female
Fishes
Oocytes
Cohen, S.
Petcoff, G.
Freijo, R.O.
Portiansky, E.L.
Barbeito, C.G.
Macchi, G.J.
Díaz, A.O.
Histochemical characterization of oocytes in the pink Cuskeel (Genypterus blacodes)
topic_facet Genypterus blacodes
Glycoconjugates
Histochemistry
Lectins
Oocytes
Genypterus blacodes
Radiata
Teleostei
animal
cytology
female
fish
oocyte
physiology
Animals
Female
Fishes
Oocytes
description In the present study we histochemically and lectinhistochemically characterized the growing oocytes of the pink cuskeel (Genypterus blacodes). We used histochemical methods for the localization and characterization of glycoconjugates (GCs) and lectin histochemical techniques for the identification of specific sugar residues. We analyzed presence and distribution of GCs in the different structures of the growing follicles (cortical alveoli, globules, yolk granules and zona radiata). During the initial stage of vitellogenesis, the oocytes presented small yolk granules composed of GCs that gradually increased during exogenous vitellogenesis. These GCs contained moderate quantities of α-D-mannose, D-glucose, N-acetylglucosamine and N-acetyl-neuraminic acid. The cortical alveoli contained both neutral and carboxylated GCs, and lectin techniques detected N-acetylgalactosamine, galactose and L-fucose. The zona radiata showed a strong positive reaction to PAS and it reacted weakly with more specific techniques, such as KOH/PAS and PA/Bh/KOH/PAS. This structure showed GCs with oxidizable vicinal diols, O-acyl sugars and sialic acid residues with different substitution types and presented N-acetylgalactosamine and L-fucose specific residues. The oocytes follicular envelope evidenced neutral and acidic non-sulfated GCs and high concentrations of α-D-mannose, D-glucose, galactose and N-acetylgalactosamine. The intergranular cytoplasmic GCs were mainly rich in α-D-mannose, D-glucose, N-acetylgalactosamine, N-acetylglucosamine and N-acetyl-neuraminic acid. These results enhance the comprehension of the structure and functionality of the pink cuskeel ovarian follicles, and provide a useful tool for the study of this tissue in other teleost species. © 2015 Zoological Society of Japan.
format JOUR
author Cohen, S.
Petcoff, G.
Freijo, R.O.
Portiansky, E.L.
Barbeito, C.G.
Macchi, G.J.
Díaz, A.O.
author_facet Cohen, S.
Petcoff, G.
Freijo, R.O.
Portiansky, E.L.
Barbeito, C.G.
Macchi, G.J.
Díaz, A.O.
author_sort Cohen, S.
title Histochemical characterization of oocytes in the pink Cuskeel (Genypterus blacodes)
title_short Histochemical characterization of oocytes in the pink Cuskeel (Genypterus blacodes)
title_full Histochemical characterization of oocytes in the pink Cuskeel (Genypterus blacodes)
title_fullStr Histochemical characterization of oocytes in the pink Cuskeel (Genypterus blacodes)
title_full_unstemmed Histochemical characterization of oocytes in the pink Cuskeel (Genypterus blacodes)
title_sort histochemical characterization of oocytes in the pink cuskeel (genypterus blacodes)
url http://hdl.handle.net/20.500.12110/paper_02890003_v32_n4_p383_Cohen
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AT freijoro histochemicalcharacterizationofoocytesinthepinkcuskeelgenypterusblacodes
AT portianskyel histochemicalcharacterizationofoocytesinthepinkcuskeelgenypterusblacodes
AT barbeitocg histochemicalcharacterizationofoocytesinthepinkcuskeelgenypterusblacodes
AT macchigj histochemicalcharacterizationofoocytesinthepinkcuskeelgenypterusblacodes
AT diazao histochemicalcharacterizationofoocytesinthepinkcuskeelgenypterusblacodes
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