Isolation, partial characterization and biological activity of mannosyl glycopeptides from seminal plasma

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Affinity chromatography on Concanavalin-A Sepharose, followed by gel filtration and hydrophobic interaction chromatography, permits the isolation of low molecular weight N-glycosidically linked oligomannosidic glycopeptides (MGp) from the autoproteolysis products of human seminal plasma. The monosac...

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Autores principales:	Lopes, C.H.G.L., Mazzini, M.N., Tortorella, H., Konrath, R.A., Brandelli, A.
Formato:	JOUR
Materias:	Carbohydrate Exocytosis Glycoconjugate Human Semen Sperm glycopeptide mannose acrosome affinity chromatography article carbohydrate analysis exocytosis human male normal human priority journal seminal plasma Acrosome Reaction Carbohydrate Conformation Chromatography, Affinity Chromatography, Gas Chromatography, Gel Glycopeptides Humans Male Mannose Oligosaccharides
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_02820080_v15_n5_p477_Lopes
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_02820080_v15_n5_p477_Lopes
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spelling	todo:paper_02820080_v15_n5_p477_Lopes2023-10-03T15:17:13Z Isolation, partial characterization and biological activity of mannosyl glycopeptides from seminal plasma Lopes, C.H.G.L. Mazzini, M.N. Tortorella, H. Konrath, R.A. Brandelli, A. Carbohydrate Exocytosis Glycoconjugate Human Semen Sperm glycopeptide mannose acrosome affinity chromatography article carbohydrate analysis exocytosis human male normal human priority journal seminal plasma Acrosome Reaction Carbohydrate Conformation Chromatography, Affinity Chromatography, Gas Chromatography, Gel Exocytosis Glycopeptides Humans Male Mannose Oligosaccharides Semen Affinity chromatography on Concanavalin-A Sepharose, followed by gel filtration and hydrophobic interaction chromatography, permits the isolation of low molecular weight N-glycosidically linked oligomannosidic glycopeptides (MGp) from the autoproteolysis products of human seminal plasma. The monosaccharide composition of MGp showed only mannose, N-acetylglucosamine and a small amount of galactose. Structural studies were carried out by methylation analysis and chromium trioxide oxidation, and results were consistent with the structures accepted for high-mannose N-glycans. MGp was capable of inhibiting the sperm acrosomal exocytosis mediated by sperm- surface receptors. These data suggest that MGp act as a 'decapacitation' factor preventing premature sperm exocytosis. Fil:Mazzini, M.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Tortorella, H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Brandelli, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_02820080_v15_n5_p477_Lopes
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Carbohydrate Exocytosis Glycoconjugate Human Semen Sperm glycopeptide mannose acrosome affinity chromatography article carbohydrate analysis exocytosis human male normal human priority journal seminal plasma Acrosome Reaction Carbohydrate Conformation Chromatography, Affinity Chromatography, Gas Chromatography, Gel Exocytosis Glycopeptides Humans Male Mannose Oligosaccharides Semen
spellingShingle	Carbohydrate Exocytosis Glycoconjugate Human Semen Sperm glycopeptide mannose acrosome affinity chromatography article carbohydrate analysis exocytosis human male normal human priority journal seminal plasma Acrosome Reaction Carbohydrate Conformation Chromatography, Affinity Chromatography, Gas Chromatography, Gel Exocytosis Glycopeptides Humans Male Mannose Oligosaccharides Semen Lopes, C.H.G.L. Mazzini, M.N. Tortorella, H. Konrath, R.A. Brandelli, A. Isolation, partial characterization and biological activity of mannosyl glycopeptides from seminal plasma
topic_facet	Carbohydrate Exocytosis Glycoconjugate Human Semen Sperm glycopeptide mannose acrosome affinity chromatography article carbohydrate analysis exocytosis human male normal human priority journal seminal plasma Acrosome Reaction Carbohydrate Conformation Chromatography, Affinity Chromatography, Gas Chromatography, Gel Exocytosis Glycopeptides Humans Male Mannose Oligosaccharides Semen
description	Affinity chromatography on Concanavalin-A Sepharose, followed by gel filtration and hydrophobic interaction chromatography, permits the isolation of low molecular weight N-glycosidically linked oligomannosidic glycopeptides (MGp) from the autoproteolysis products of human seminal plasma. The monosaccharide composition of MGp showed only mannose, N-acetylglucosamine and a small amount of galactose. Structural studies were carried out by methylation analysis and chromium trioxide oxidation, and results were consistent with the structures accepted for high-mannose N-glycans. MGp was capable of inhibiting the sperm acrosomal exocytosis mediated by sperm- surface receptors. These data suggest that MGp act as a 'decapacitation' factor preventing premature sperm exocytosis.
format	JOUR
author	Lopes, C.H.G.L. Mazzini, M.N. Tortorella, H. Konrath, R.A. Brandelli, A.
author_facet	Lopes, C.H.G.L. Mazzini, M.N. Tortorella, H. Konrath, R.A. Brandelli, A.
author_sort	Lopes, C.H.G.L.
title	Isolation, partial characterization and biological activity of mannosyl glycopeptides from seminal plasma
title_short	Isolation, partial characterization and biological activity of mannosyl glycopeptides from seminal plasma
title_full	Isolation, partial characterization and biological activity of mannosyl glycopeptides from seminal plasma
title_fullStr	Isolation, partial characterization and biological activity of mannosyl glycopeptides from seminal plasma
title_full_unstemmed	Isolation, partial characterization and biological activity of mannosyl glycopeptides from seminal plasma
title_sort	isolation, partial characterization and biological activity of mannosyl glycopeptides from seminal plasma
url	http://hdl.handle.net/20.500.12110/paper_02820080_v15_n5_p477_Lopes
work_keys_str_mv	AT lopeschgl isolationpartialcharacterizationandbiologicalactivityofmannosylglycopeptidesfromseminalplasma AT mazzinimn isolationpartialcharacterizationandbiologicalactivityofmannosylglycopeptidesfromseminalplasma AT tortorellah isolationpartialcharacterizationandbiologicalactivityofmannosylglycopeptidesfromseminalplasma AT konrathra isolationpartialcharacterizationandbiologicalactivityofmannosylglycopeptidesfromseminalplasma AT brandellia isolationpartialcharacterizationandbiologicalactivityofmannosylglycopeptidesfromseminalplasma
_version_	1807319922681839616

Isolation, partial characterization and biological activity of mannosyl glycopeptides from seminal plasma

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