Glucocorticoid regulation of motoneuronal parameters in rats with spinal cord injury
1. Glucocorticoids exert beneficial effects after acute CNS injury in humans and experimental animals. To elucidate potential mechanisms of glucocorticoid action in the lesioned spinal cord, we have studied if treatment with dexamethasone (DEX) modulated the neurotrophin binding receptor p75 (p75(NT...
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todo:paper_02724340_v19_n5_p597_Gonzalez2023-10-03T15:14:59Z Glucocorticoid regulation of motoneuronal parameters in rats with spinal cord injury Gonzalez, S.L. Saravia, F. Gonzalez Deniselle, M.C. Lima, A.E. De Nicola, A.F. Choline acetyltransferase Glucocorticoids Motoneurons Neuroprotection p75 neurotrophin receptor Spinal cord transection dexamethasone glucocorticoid neurotropin protein p75 unclassified drug animal cell antiinflammatory activity article controlled study densitometry drug mechanism immunoreactivity male neuroprotection nonhuman priority journal quantitative assay rat spinal cord injury spinal cord motoneuron steroid metabolism subcutaneous drug administration Animals Choline O-Acetyltransferase Dexamethasone Glucocorticoids Male Motor Neurons Rats Rats, Sprague-Dawley Receptor, Nerve Growth Factor Receptors, Nerve Growth Factor Spinal Cord Injuries Animalia 1. Glucocorticoids exert beneficial effects after acute CNS injury in humans and experimental animals. To elucidate potential mechanisms of glucocorticoid action in the lesioned spinal cord, we have studied if treatment with dexamethasone (DEX) modulated the neurotrophin binding receptor p75 (p75(NTR)) and choline acetyltransferase (ChAT), a marker of neuronal functional viability. 2. Rats with a sham operation or with spinal cord transection at the thoracic level received vehicle or DEX several times postlesion and were sacrificed 48 hr after surgery. The lumbar region caudal to the lesion was processed for p75(NTR) and ChAT immunoreactivity (IR) using quantitative densitometric analysis. 3. We observed that p75(NTR)-IR was absent from ventral horn motoneurons of sham-operated rats, in contrast to strong staining of neuronal perikaryon in TRX rats. Administration of DEX to TRX rats had no effect on the number of neuronal cell bodies expressing p75(NTR)-IR but significantly increased the number and length of immunostained neuronal processes. 4. Furthermore, spinal cord transection reduced ChAT immunostaining of motoneurons by 50%, whereas DEX treatment reverted this pattern to cells with a strong immunoreaction intensity in perikaryon and cell processes. 5. It is hypothesized that increased expression of p75(NTR) in cell processes and of ChAT in motoneurons may be part of a mechanism by which glucocorticoids afford neuroprotection, in addition to their known antiinflammatory effects. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_02724340_v19_n5_p597_Gonzalez |
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Universidad de Buenos Aires |
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I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Choline acetyltransferase Glucocorticoids Motoneurons Neuroprotection p75 neurotrophin receptor Spinal cord transection dexamethasone glucocorticoid neurotropin protein p75 unclassified drug animal cell antiinflammatory activity article controlled study densitometry drug mechanism immunoreactivity male neuroprotection nonhuman priority journal quantitative assay rat spinal cord injury spinal cord motoneuron steroid metabolism subcutaneous drug administration Animals Choline O-Acetyltransferase Dexamethasone Glucocorticoids Male Motor Neurons Rats Rats, Sprague-Dawley Receptor, Nerve Growth Factor Receptors, Nerve Growth Factor Spinal Cord Injuries Animalia |
| spellingShingle |
Choline acetyltransferase Glucocorticoids Motoneurons Neuroprotection p75 neurotrophin receptor Spinal cord transection dexamethasone glucocorticoid neurotropin protein p75 unclassified drug animal cell antiinflammatory activity article controlled study densitometry drug mechanism immunoreactivity male neuroprotection nonhuman priority journal quantitative assay rat spinal cord injury spinal cord motoneuron steroid metabolism subcutaneous drug administration Animals Choline O-Acetyltransferase Dexamethasone Glucocorticoids Male Motor Neurons Rats Rats, Sprague-Dawley Receptor, Nerve Growth Factor Receptors, Nerve Growth Factor Spinal Cord Injuries Animalia Gonzalez, S.L. Saravia, F. Gonzalez Deniselle, M.C. Lima, A.E. De Nicola, A.F. Glucocorticoid regulation of motoneuronal parameters in rats with spinal cord injury |
| topic_facet |
Choline acetyltransferase Glucocorticoids Motoneurons Neuroprotection p75 neurotrophin receptor Spinal cord transection dexamethasone glucocorticoid neurotropin protein p75 unclassified drug animal cell antiinflammatory activity article controlled study densitometry drug mechanism immunoreactivity male neuroprotection nonhuman priority journal quantitative assay rat spinal cord injury spinal cord motoneuron steroid metabolism subcutaneous drug administration Animals Choline O-Acetyltransferase Dexamethasone Glucocorticoids Male Motor Neurons Rats Rats, Sprague-Dawley Receptor, Nerve Growth Factor Receptors, Nerve Growth Factor Spinal Cord Injuries Animalia |
| description |
1. Glucocorticoids exert beneficial effects after acute CNS injury in humans and experimental animals. To elucidate potential mechanisms of glucocorticoid action in the lesioned spinal cord, we have studied if treatment with dexamethasone (DEX) modulated the neurotrophin binding receptor p75 (p75(NTR)) and choline acetyltransferase (ChAT), a marker of neuronal functional viability. 2. Rats with a sham operation or with spinal cord transection at the thoracic level received vehicle or DEX several times postlesion and were sacrificed 48 hr after surgery. The lumbar region caudal to the lesion was processed for p75(NTR) and ChAT immunoreactivity (IR) using quantitative densitometric analysis. 3. We observed that p75(NTR)-IR was absent from ventral horn motoneurons of sham-operated rats, in contrast to strong staining of neuronal perikaryon in TRX rats. Administration of DEX to TRX rats had no effect on the number of neuronal cell bodies expressing p75(NTR)-IR but significantly increased the number and length of immunostained neuronal processes. 4. Furthermore, spinal cord transection reduced ChAT immunostaining of motoneurons by 50%, whereas DEX treatment reverted this pattern to cells with a strong immunoreaction intensity in perikaryon and cell processes. 5. It is hypothesized that increased expression of p75(NTR) in cell processes and of ChAT in motoneurons may be part of a mechanism by which glucocorticoids afford neuroprotection, in addition to their known antiinflammatory effects. |
| format |
JOUR |
| author |
Gonzalez, S.L. Saravia, F. Gonzalez Deniselle, M.C. Lima, A.E. De Nicola, A.F. |
| author_facet |
Gonzalez, S.L. Saravia, F. Gonzalez Deniselle, M.C. Lima, A.E. De Nicola, A.F. |
| author_sort |
Gonzalez, S.L. |
| title |
Glucocorticoid regulation of motoneuronal parameters in rats with spinal cord injury |
| title_short |
Glucocorticoid regulation of motoneuronal parameters in rats with spinal cord injury |
| title_full |
Glucocorticoid regulation of motoneuronal parameters in rats with spinal cord injury |
| title_fullStr |
Glucocorticoid regulation of motoneuronal parameters in rats with spinal cord injury |
| title_full_unstemmed |
Glucocorticoid regulation of motoneuronal parameters in rats with spinal cord injury |
| title_sort |
glucocorticoid regulation of motoneuronal parameters in rats with spinal cord injury |
| url |
http://hdl.handle.net/20.500.12110/paper_02724340_v19_n5_p597_Gonzalez |
| work_keys_str_mv |
AT gonzalezsl glucocorticoidregulationofmotoneuronalparametersinratswithspinalcordinjury AT saraviaf glucocorticoidregulationofmotoneuronalparametersinratswithspinalcordinjury AT gonzalezdenisellemc glucocorticoidregulationofmotoneuronalparametersinratswithspinalcordinjury AT limaae glucocorticoidregulationofmotoneuronalparametersinratswithspinalcordinjury AT denicolaaf glucocorticoidregulationofmotoneuronalparametersinratswithspinalcordinjury |
| _version_ |
1782024067697606656 |