Immunohistochemical Localization of Concanavalin A and Wheat Germ Lectin Receptors in the Normal Human Spermatozoa

ABSTRACT: A semiquantitative immunohistochemical technique for the detection of N‐acetyl α‐D‐neuraminic acid, N‐acetyl β‐D‐glucosamine and its β‐(1 ŕ 4)‐linked internal chains, α‐D‐glucopyranosyl and α‐D‐mannopyranosyl and its α‐(1 ŕ 2)‐linked internal chains and sterically related, nonreducing, end...

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Autores principales: DE CEREZO, J.M.S., BUENO, M.P., SKOWRONKI, B., CEREZO, A.S.
Formato: JOUR
Materias:
Human spermatozoa
lectin receptors
membrane surfaces
spermatozoal antigens
concanavalin a
lectin receptor
membrane receptor
sperm antigen
wheat germ agglutinin
cytology
drug protein binding
human
human cell
immunohistochemistry
male genital system
Cell Membrane
Human
Immunologic Techniques
Lectins
Male
Receptors, Concanavalin A
Receptors, Mitogen
Spermatozoa
Support, Non-U.S. Gov't
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_02717352_v2_n5_p246_DECEREZO
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_02717352_v2_n5_p246_DECEREZO
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spelling todo:paper_02717352_v2_n5_p246_DECEREZO2023-10-03T15:14:53Z Immunohistochemical Localization of Concanavalin A and Wheat Germ Lectin Receptors in the Normal Human Spermatozoa DE CEREZO, J.M.S. BUENO, M.P. SKOWRONKI, B. CEREZO, A.S. Human spermatozoa lectin receptors membrane surfaces spermatozoal antigens concanavalin a lectin receptor membrane receptor sperm antigen wheat germ agglutinin cytology drug protein binding human human cell immunohistochemistry male genital system Cell Membrane Human Immunologic Techniques Lectins Male Receptors, Concanavalin A Receptors, Mitogen Spermatozoa Support, Non-U.S. Gov't ABSTRACT: A semiquantitative immunohistochemical technique for the detection of N‐acetyl α‐D‐neuraminic acid, N‐acetyl β‐D‐glucosamine and its β‐(1 ŕ 4)‐linked internal chains, α‐D‐glucopyranosyl and α‐D‐mannopyranosyl and its α‐(1 ŕ 2)‐linked internal chains and sterically related, nonreducing, end‐chain residues of oligosaccharide chains of glycoproteins or glycolipids on the surface of membranes was developed using Con A and wheat germ lectins. When this method was applied to the localization of carbohydrate receptors on the membrane of the normal human spermatozoa, it was found that the Con A and wheat germ lectin receptors were mainly located in the equatorial and post nuclear cap with few receptors located in the acrosome and neck. None of them were found in the intermediate segment plus tail. Con A receptors were α‐D‐mannopyranosyl end‐chain residues and wheat germ lectin receptors were N‐acetyl β‐D‐glucosamine (1 ŕ 4)‐linked internal chains. These groups occur together in the oligomannosidic type of N‐glycosidic‐linked oligosaccharide chains of glycoproteins and so the use of both lectins on desialycated membranes or on those which contain nonclustered N‐acetyl neuraminic acid residues may be of help to localize this type of glycoprotein oligosaccharide chains. Con A receptors were not removed after proteases digestion, suggesting the possibility that they are part of intrinsic spermatozoal antigens. 1982 Munksgaard JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_02717352_v2_n5_p246_DECEREZO
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Human spermatozoa
lectin receptors
membrane surfaces
spermatozoal antigens
concanavalin a
lectin receptor
membrane receptor
sperm antigen
wheat germ agglutinin
cytology
drug protein binding
human
human cell
immunohistochemistry
male genital system
Cell Membrane
Human
Immunologic Techniques
Lectins
Male
Receptors, Concanavalin A
Receptors, Mitogen
Spermatozoa
Support, Non-U.S. Gov't
spellingShingle Human spermatozoa
lectin receptors
membrane surfaces
spermatozoal antigens
concanavalin a
lectin receptor
membrane receptor
sperm antigen
wheat germ agglutinin
cytology
drug protein binding
human
human cell
immunohistochemistry
male genital system
Cell Membrane
Human
Immunologic Techniques
Lectins
Male
Receptors, Concanavalin A
Receptors, Mitogen
Spermatozoa
Support, Non-U.S. Gov't
DE CEREZO, J.M.S.
BUENO, M.P.
SKOWRONKI, B.
CEREZO, A.S.
Immunohistochemical Localization of Concanavalin A and Wheat Germ Lectin Receptors in the Normal Human Spermatozoa
topic_facet Human spermatozoa
lectin receptors
membrane surfaces
spermatozoal antigens
concanavalin a
lectin receptor
membrane receptor
sperm antigen
wheat germ agglutinin
cytology
drug protein binding
human
human cell
immunohistochemistry
male genital system
Cell Membrane
Human
Immunologic Techniques
Lectins
Male
Receptors, Concanavalin A
Receptors, Mitogen
Spermatozoa
Support, Non-U.S. Gov't
description ABSTRACT: A semiquantitative immunohistochemical technique for the detection of N‐acetyl α‐D‐neuraminic acid, N‐acetyl β‐D‐glucosamine and its β‐(1 ŕ 4)‐linked internal chains, α‐D‐glucopyranosyl and α‐D‐mannopyranosyl and its α‐(1 ŕ 2)‐linked internal chains and sterically related, nonreducing, end‐chain residues of oligosaccharide chains of glycoproteins or glycolipids on the surface of membranes was developed using Con A and wheat germ lectins. When this method was applied to the localization of carbohydrate receptors on the membrane of the normal human spermatozoa, it was found that the Con A and wheat germ lectin receptors were mainly located in the equatorial and post nuclear cap with few receptors located in the acrosome and neck. None of them were found in the intermediate segment plus tail. Con A receptors were α‐D‐mannopyranosyl end‐chain residues and wheat germ lectin receptors were N‐acetyl β‐D‐glucosamine (1 ŕ 4)‐linked internal chains. These groups occur together in the oligomannosidic type of N‐glycosidic‐linked oligosaccharide chains of glycoproteins and so the use of both lectins on desialycated membranes or on those which contain nonclustered N‐acetyl neuraminic acid residues may be of help to localize this type of glycoprotein oligosaccharide chains. Con A receptors were not removed after proteases digestion, suggesting the possibility that they are part of intrinsic spermatozoal antigens. 1982 Munksgaard
format JOUR
author DE CEREZO, J.M.S.
BUENO, M.P.
SKOWRONKI, B.
CEREZO, A.S.
author_facet DE CEREZO, J.M.S.
BUENO, M.P.
SKOWRONKI, B.
CEREZO, A.S.
author_sort DE CEREZO, J.M.S.
title Immunohistochemical Localization of Concanavalin A and Wheat Germ Lectin Receptors in the Normal Human Spermatozoa
title_short Immunohistochemical Localization of Concanavalin A and Wheat Germ Lectin Receptors in the Normal Human Spermatozoa
title_full Immunohistochemical Localization of Concanavalin A and Wheat Germ Lectin Receptors in the Normal Human Spermatozoa
title_fullStr Immunohistochemical Localization of Concanavalin A and Wheat Germ Lectin Receptors in the Normal Human Spermatozoa
title_full_unstemmed Immunohistochemical Localization of Concanavalin A and Wheat Germ Lectin Receptors in the Normal Human Spermatozoa
title_sort immunohistochemical localization of concanavalin a and wheat germ lectin receptors in the normal human spermatozoa
url http://hdl.handle.net/20.500.12110/paper_02717352_v2_n5_p246_DECEREZO
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AT buenomp immunohistochemicallocalizationofconcanavalinaandwheatgermlectinreceptorsinthenormalhumanspermatozoa
AT skowronkib immunohistochemicallocalizationofconcanavalinaandwheatgermlectinreceptorsinthenormalhumanspermatozoa
AT cerezoas immunohistochemicallocalizationofconcanavalinaandwheatgermlectinreceptorsinthenormalhumanspermatozoa
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