Study of phenformin metabolism in rat liver microsomes by HPLC, CE and on-line HPLC-electrospray ionization mass spectrometry

Methods for the analysis of phenformin and its metabolite by high-performance liquid chromatography (HPLC), capillary electrophoresis (CE) and high-performance liquid chromatography-electrospray ionization mass spectrometry (HPLC-ESIMS) are developed. The effects of pH, buffer concentration and prop...

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Autores principales:	Llambias, E.B.C., Luo, J.
Formato:	JOUR
Materias:	phenformin animal cell article capillary electrophoresis concentration response controlled study drug metabolism high performance liquid chromatography incubation time ionization liver microsome mass spectrometry metabolic inhibition metabolite nonhuman ph rat Acetates Animals Buffers Chromatography, High Pressure Liquid Electrophoresis, Capillary Female Hydrogen-Ion Concentration Hypoglycemic Agents Mass Spectrometry Metformin Methanol Microsomes, Liver Online Systems Osmolar Concentration Phenformin Rats Spectrophotometry, Ultraviolet Time Factors Animalia
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_02693879_v10_n4_p155_Llambias
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_02693879_v10_n4_p155_Llambias
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spelling	todo:paper_02693879_v10_n4_p155_Llambias2023-10-03T15:14:11Z Study of phenformin metabolism in rat liver microsomes by HPLC, CE and on-line HPLC-electrospray ionization mass spectrometry Llambias, E.B.C. Luo, J. phenformin animal cell article capillary electrophoresis concentration response controlled study drug metabolism high performance liquid chromatography incubation time ionization liver microsome mass spectrometry metabolic inhibition metabolite nonhuman ph rat Acetates Animals Buffers Chromatography, High Pressure Liquid Electrophoresis, Capillary Female Hydrogen-Ion Concentration Hypoglycemic Agents Mass Spectrometry Metformin Methanol Microsomes, Liver Online Systems Osmolar Concentration Phenformin Rats Spectrophotometry, Ultraviolet Time Factors Animalia Methods for the analysis of phenformin and its metabolite by high-performance liquid chromatography (HPLC), capillary electrophoresis (CE) and high-performance liquid chromatography-electrospray ionization mass spectrometry (HPLC-ESIMS) are developed. The effects of pH, buffer concentration and proportion of organic modifier on the retention of the compounds in HPLC have been studied. The optimum condition was used for the separation and identification of phenformin and its metabolite in microsomal metabolism by HPLC-ESIMS. A simple CE method is also described for the separation of these compounds. Optimum incubation conditions and cofactor requirements for the formation of 4-hydroxyphenformin by microsomal preparations of rat liver were determined. A linear response in the formation of product was found with increasing concentrations of protein and up to 15 min incubation. High concentrations of phenformin inhibited its metabolite formation, and K(m) was 4 μM. Fil:Llambias, E.B.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_02693879_v10_n4_p155_Llambias
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	phenformin animal cell article capillary electrophoresis concentration response controlled study drug metabolism high performance liquid chromatography incubation time ionization liver microsome mass spectrometry metabolic inhibition metabolite nonhuman ph rat Acetates Animals Buffers Chromatography, High Pressure Liquid Electrophoresis, Capillary Female Hydrogen-Ion Concentration Hypoglycemic Agents Mass Spectrometry Metformin Methanol Microsomes, Liver Online Systems Osmolar Concentration Phenformin Rats Spectrophotometry, Ultraviolet Time Factors Animalia
spellingShingle	phenformin animal cell article capillary electrophoresis concentration response controlled study drug metabolism high performance liquid chromatography incubation time ionization liver microsome mass spectrometry metabolic inhibition metabolite nonhuman ph rat Acetates Animals Buffers Chromatography, High Pressure Liquid Electrophoresis, Capillary Female Hydrogen-Ion Concentration Hypoglycemic Agents Mass Spectrometry Metformin Methanol Microsomes, Liver Online Systems Osmolar Concentration Phenformin Rats Spectrophotometry, Ultraviolet Time Factors Animalia Llambias, E.B.C. Luo, J. Study of phenformin metabolism in rat liver microsomes by HPLC, CE and on-line HPLC-electrospray ionization mass spectrometry
topic_facet	phenformin animal cell article capillary electrophoresis concentration response controlled study drug metabolism high performance liquid chromatography incubation time ionization liver microsome mass spectrometry metabolic inhibition metabolite nonhuman ph rat Acetates Animals Buffers Chromatography, High Pressure Liquid Electrophoresis, Capillary Female Hydrogen-Ion Concentration Hypoglycemic Agents Mass Spectrometry Metformin Methanol Microsomes, Liver Online Systems Osmolar Concentration Phenformin Rats Spectrophotometry, Ultraviolet Time Factors Animalia
description	Methods for the analysis of phenformin and its metabolite by high-performance liquid chromatography (HPLC), capillary electrophoresis (CE) and high-performance liquid chromatography-electrospray ionization mass spectrometry (HPLC-ESIMS) are developed. The effects of pH, buffer concentration and proportion of organic modifier on the retention of the compounds in HPLC have been studied. The optimum condition was used for the separation and identification of phenformin and its metabolite in microsomal metabolism by HPLC-ESIMS. A simple CE method is also described for the separation of these compounds. Optimum incubation conditions and cofactor requirements for the formation of 4-hydroxyphenformin by microsomal preparations of rat liver were determined. A linear response in the formation of product was found with increasing concentrations of protein and up to 15 min incubation. High concentrations of phenformin inhibited its metabolite formation, and K(m) was 4 μM.
format	JOUR
author	Llambias, E.B.C. Luo, J.
author_facet	Llambias, E.B.C. Luo, J.
author_sort	Llambias, E.B.C.
title	Study of phenformin metabolism in rat liver microsomes by HPLC, CE and on-line HPLC-electrospray ionization mass spectrometry
title_short	Study of phenformin metabolism in rat liver microsomes by HPLC, CE and on-line HPLC-electrospray ionization mass spectrometry
title_full	Study of phenformin metabolism in rat liver microsomes by HPLC, CE and on-line HPLC-electrospray ionization mass spectrometry
title_fullStr	Study of phenformin metabolism in rat liver microsomes by HPLC, CE and on-line HPLC-electrospray ionization mass spectrometry
title_full_unstemmed	Study of phenformin metabolism in rat liver microsomes by HPLC, CE and on-line HPLC-electrospray ionization mass spectrometry
title_sort	study of phenformin metabolism in rat liver microsomes by hplc, ce and on-line hplc-electrospray ionization mass spectrometry
url	http://hdl.handle.net/20.500.12110/paper_02693879_v10_n4_p155_Llambias
work_keys_str_mv	AT llambiasebc studyofphenforminmetabolisminratlivermicrosomesbyhplcceandonlinehplcelectrosprayionizationmassspectrometry AT luoj studyofphenforminmetabolisminratlivermicrosomesbyhplcceandonlinehplcelectrosprayionizationmassspectrometry
_version_	1782023973809160192

Study of phenformin metabolism in rat liver microsomes by HPLC, CE and on-line HPLC-electrospray ionization mass spectrometry

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