Relationship of ovarian stimulation response with vascular endothelial growth factor and degree of granulosa cell apoptosis
Background: The aim of this study was to evaluate the concentration of vascular endothelial growth factor (VEGF) in follicular fluid and in granulosa cell cultures in relation to the degree of apoptosis in granulosa cells from patients with different types of ovarian response to controlled ovarian h...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_02681161_v16_n9_p1814_Quintana |
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todo:paper_02681161_v16_n9_p1814_Quintana2023-10-03T15:13:54Z Relationship of ovarian stimulation response with vascular endothelial growth factor and degree of granulosa cell apoptosis Quintana, R. Kopcow, L. Marconi, G. Sueldo, C. Speranza, G. Barañao, R.I. Apoptosis Controlled ovarian hyperstimulation (COH) Granulosa cells (GC) IVF Vascular endothelial growth factor (VEGF) chorionic gonadotropin human menopausal gonadotropin leuprorelin recombinant follitropin vasculotropin adult apoptosis article cell culture clinical article concentration response controlled study dose response female female infertility follicle fluid granular cell human oocyte ovary hyperstimulation ovulation induction supernatant Background: The aim of this study was to evaluate the concentration of vascular endothelial growth factor (VEGF) in follicular fluid and in granulosa cell cultures in relation to the degree of apoptosis in granulosa cells from patients with different types of ovarian response to controlled ovarian hyperstimulation. Methods: We studied 30 women who underwent controlled ovarian hyperstimulation and oocyte retrieval. Group A comprised patients with 1-4 follicles (n = 10), group B patients with 5-14 follicles (n = 10) and group C patients with >15 follicles (n = 10). Results: Mean (±SD) VEGF concentrations in follicular fluid were 1232 ± 209, 813 ± 198 and 396 ± 103 pg/ml for groups A, B and C respectively (P > 0.01). Concentrations of VEGF in granulosa cell supernatant were 684 ± 316, 1101 ± 295 and 1596 ± 227 pg/ml respectively (P < 0.05). Percentages of apoptotic cells in granulosa cells culture was 55.02 ± 7.5, 23.98 ± 4.4 and 14.2 ± 2.3% respectively (A versus B, P < 0.01, A versus C, P < 0.006, B versus C, NS). Conclusions: Our findings showed that in patients with decreased ovarian response to controlled ovarian hyperstimulation, follicular fluid VEGF concentration is elevated, the concentration from granulosa cells culture supernatant is decreased and the percentage of apoptotic granulosa cells is increased, while opposite findings occurred in patients with normal or hyper-responses. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_02681161_v16_n9_p1814_Quintana |
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Universidad de Buenos Aires |
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I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Apoptosis Controlled ovarian hyperstimulation (COH) Granulosa cells (GC) IVF Vascular endothelial growth factor (VEGF) chorionic gonadotropin human menopausal gonadotropin leuprorelin recombinant follitropin vasculotropin adult apoptosis article cell culture clinical article concentration response controlled study dose response female female infertility follicle fluid granular cell human oocyte ovary hyperstimulation ovulation induction supernatant |
| spellingShingle |
Apoptosis Controlled ovarian hyperstimulation (COH) Granulosa cells (GC) IVF Vascular endothelial growth factor (VEGF) chorionic gonadotropin human menopausal gonadotropin leuprorelin recombinant follitropin vasculotropin adult apoptosis article cell culture clinical article concentration response controlled study dose response female female infertility follicle fluid granular cell human oocyte ovary hyperstimulation ovulation induction supernatant Quintana, R. Kopcow, L. Marconi, G. Sueldo, C. Speranza, G. Barañao, R.I. Relationship of ovarian stimulation response with vascular endothelial growth factor and degree of granulosa cell apoptosis |
| topic_facet |
Apoptosis Controlled ovarian hyperstimulation (COH) Granulosa cells (GC) IVF Vascular endothelial growth factor (VEGF) chorionic gonadotropin human menopausal gonadotropin leuprorelin recombinant follitropin vasculotropin adult apoptosis article cell culture clinical article concentration response controlled study dose response female female infertility follicle fluid granular cell human oocyte ovary hyperstimulation ovulation induction supernatant |
| description |
Background: The aim of this study was to evaluate the concentration of vascular endothelial growth factor (VEGF) in follicular fluid and in granulosa cell cultures in relation to the degree of apoptosis in granulosa cells from patients with different types of ovarian response to controlled ovarian hyperstimulation. Methods: We studied 30 women who underwent controlled ovarian hyperstimulation and oocyte retrieval. Group A comprised patients with 1-4 follicles (n = 10), group B patients with 5-14 follicles (n = 10) and group C patients with >15 follicles (n = 10). Results: Mean (±SD) VEGF concentrations in follicular fluid were 1232 ± 209, 813 ± 198 and 396 ± 103 pg/ml for groups A, B and C respectively (P > 0.01). Concentrations of VEGF in granulosa cell supernatant were 684 ± 316, 1101 ± 295 and 1596 ± 227 pg/ml respectively (P < 0.05). Percentages of apoptotic cells in granulosa cells culture was 55.02 ± 7.5, 23.98 ± 4.4 and 14.2 ± 2.3% respectively (A versus B, P < 0.01, A versus C, P < 0.006, B versus C, NS). Conclusions: Our findings showed that in patients with decreased ovarian response to controlled ovarian hyperstimulation, follicular fluid VEGF concentration is elevated, the concentration from granulosa cells culture supernatant is decreased and the percentage of apoptotic granulosa cells is increased, while opposite findings occurred in patients with normal or hyper-responses. |
| format |
JOUR |
| author |
Quintana, R. Kopcow, L. Marconi, G. Sueldo, C. Speranza, G. Barañao, R.I. |
| author_facet |
Quintana, R. Kopcow, L. Marconi, G. Sueldo, C. Speranza, G. Barañao, R.I. |
| author_sort |
Quintana, R. |
| title |
Relationship of ovarian stimulation response with vascular endothelial growth factor and degree of granulosa cell apoptosis |
| title_short |
Relationship of ovarian stimulation response with vascular endothelial growth factor and degree of granulosa cell apoptosis |
| title_full |
Relationship of ovarian stimulation response with vascular endothelial growth factor and degree of granulosa cell apoptosis |
| title_fullStr |
Relationship of ovarian stimulation response with vascular endothelial growth factor and degree of granulosa cell apoptosis |
| title_full_unstemmed |
Relationship of ovarian stimulation response with vascular endothelial growth factor and degree of granulosa cell apoptosis |
| title_sort |
relationship of ovarian stimulation response with vascular endothelial growth factor and degree of granulosa cell apoptosis |
| url |
http://hdl.handle.net/20.500.12110/paper_02681161_v16_n9_p1814_Quintana |
| work_keys_str_mv |
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