Typing clinical and animal environment Aspergillus fumigatus gliotoxin producer strains isolated from Brazil by PCR-RFLP markers

Aspergillus fumigatus, a well-known human and animal pathogen causing aspergillosis, has been historically identified by morphological and microscopic features. However, recent studies have shown that species identification on the basis of morphology alone is problematic. The aim of this work was to...

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Detalles Bibliográficos
Autores principales: Soleiro, C.A., Pena, G.A., Cavaglieri, L.R., Coelho, I., Keller, L.M., Dalcero, A.M., Rosa, C.A.R.
Formato: JOUR
Lenguaje:English
Materias:
Animal environment
Aspergillus fumigatus
Clinical (animal and human)
Restriction enzymes
Taxonomic state
BccI enzyme
gliotoxin
MspI enzyme
restriction endonuclease
Sau3AI enzyme
unclassified drug
animal
bioassay
biomarker
isolated population
maize
pathogen
polymerase chain reaction
sorghum
taxonomy
animal food
article
aspergillosis
bovine mastitis
cereal
computer model
fungal strain
fungus isolation
morphology
nucleotide sequence
productivity
restriction fragment length polymorphism
reverse transcription polymerase chain reaction
risk assessment
rural population
silage
species identification
Brazil
Animalia
Bovinae
Zea mays
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_02668254_v57_n6_p484_Soleiro
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:Aspergillus fumigatus, a well-known human and animal pathogen causing aspergillosis, has been historically identified by morphological and microscopic features. However, recent studies have shown that species identification on the basis of morphology alone is problematic. The aim of this work was to confirm the taxonomic state at specie level of a set of clinical (human and animal) and animal environment A. fumigatus strains identified by morphological criteria applying a PCR-RFLP assay by an in silico and in situ analysis with three restriction enzymes. The A. fumigatus gliotoxin-producing ability was also determined. Previous to the in situ PCR-RFLP analysis, an in silico assay with BccI, MspI and Sau3AI restriction enzymes was carried out. After that, these enzymes were used for in situ assay. All A. fumigatus strains isolated from corn silage, human aspergillosis and bovine mastitis and high per cent of the strains isolated from cereals, animal feedstuff and sorghum silage were able to produce high gliotoxin levels. Also, all these strains identified by morphological criteria as A. fumigatus, regardless of its isolation source, had band patterns according to A. fumigatus sensu stricto by PCR-RFLP markers. © 2013 The Society for Applied Microbiology.

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