Typing clinical and animal environment Aspergillus fumigatus gliotoxin producer strains isolated from Brazil by PCR-RFLP markers

Aspergillus fumigatus, a well-known human and animal pathogen causing aspergillosis, has been historically identified by morphological and microscopic features. However, recent studies have shown that species identification on the basis of morphology alone is problematic. The aim of this work was to...

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Autores principales:	Soleiro, C.A., Pena, G.A., Cavaglieri, L.R., Coelho, I., Keller, L.M., Dalcero, A.M., Rosa, C.A.R.
Formato:	JOUR
Lenguaje:	English
Materias:	Animal environment Aspergillus fumigatus Clinical (animal and human) Restriction enzymes Taxonomic state BccI enzyme gliotoxin MspI enzyme restriction endonuclease Sau3AI enzyme unclassified drug animal bioassay biomarker isolated population maize pathogen polymerase chain reaction sorghum taxonomy animal food article aspergillosis bovine mastitis cereal computer model fungal strain fungus isolation morphology nucleotide sequence productivity restriction fragment length polymorphism reverse transcription polymerase chain reaction risk assessment rural population silage species identification Brazil Animalia Bovinae Zea mays
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_02668254_v57_n6_p484_Soleiro
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_02668254_v57_n6_p484_Soleiro
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spelling	todo:paper_02668254_v57_n6_p484_Soleiro2023-10-03T15:13:27Z Typing clinical and animal environment Aspergillus fumigatus gliotoxin producer strains isolated from Brazil by PCR-RFLP markers Soleiro, C.A. Pena, G.A. Cavaglieri, L.R. Coelho, I. Keller, L.M. Dalcero, A.M. Rosa, C.A.R. Animal environment Aspergillus fumigatus Clinical (animal and human) Restriction enzymes Taxonomic state BccI enzyme gliotoxin MspI enzyme restriction endonuclease Sau3AI enzyme unclassified drug animal bioassay biomarker isolated population maize pathogen polymerase chain reaction sorghum taxonomy animal food article aspergillosis Aspergillus fumigatus bovine mastitis cereal computer model fungal strain fungus isolation maize morphology nucleotide sequence productivity restriction fragment length polymorphism reverse transcription polymerase chain reaction risk assessment rural population silage sorghum species identification Brazil Animalia Aspergillus fumigatus Bovinae Zea mays Aspergillus fumigatus, a well-known human and animal pathogen causing aspergillosis, has been historically identified by morphological and microscopic features. However, recent studies have shown that species identification on the basis of morphology alone is problematic. The aim of this work was to confirm the taxonomic state at specie level of a set of clinical (human and animal) and animal environment A. fumigatus strains identified by morphological criteria applying a PCR-RFLP assay by an in silico and in situ analysis with three restriction enzymes. The A. fumigatus gliotoxin-producing ability was also determined. Previous to the in situ PCR-RFLP analysis, an in silico assay with BccI, MspI and Sau3AI restriction enzymes was carried out. After that, these enzymes were used for in situ assay. All A. fumigatus strains isolated from corn silage, human aspergillosis and bovine mastitis and high per cent of the strains isolated from cereals, animal feedstuff and sorghum silage were able to produce high gliotoxin levels. Also, all these strains identified by morphological criteria as A. fumigatus, regardless of its isolation source, had band patterns according to A. fumigatus sensu stricto by PCR-RFLP markers. © 2013 The Society for Applied Microbiology. JOUR English info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_02668254_v57_n6_p484_Soleiro
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
language	English
orig_language_str_mv	English
topic	Animal environment Aspergillus fumigatus Clinical (animal and human) Restriction enzymes Taxonomic state BccI enzyme gliotoxin MspI enzyme restriction endonuclease Sau3AI enzyme unclassified drug animal bioassay biomarker isolated population maize pathogen polymerase chain reaction sorghum taxonomy animal food article aspergillosis Aspergillus fumigatus bovine mastitis cereal computer model fungal strain fungus isolation maize morphology nucleotide sequence productivity restriction fragment length polymorphism reverse transcription polymerase chain reaction risk assessment rural population silage sorghum species identification Brazil Animalia Aspergillus fumigatus Bovinae Zea mays
spellingShingle	Animal environment Aspergillus fumigatus Clinical (animal and human) Restriction enzymes Taxonomic state BccI enzyme gliotoxin MspI enzyme restriction endonuclease Sau3AI enzyme unclassified drug animal bioassay biomarker isolated population maize pathogen polymerase chain reaction sorghum taxonomy animal food article aspergillosis Aspergillus fumigatus bovine mastitis cereal computer model fungal strain fungus isolation maize morphology nucleotide sequence productivity restriction fragment length polymorphism reverse transcription polymerase chain reaction risk assessment rural population silage sorghum species identification Brazil Animalia Aspergillus fumigatus Bovinae Zea mays Soleiro, C.A. Pena, G.A. Cavaglieri, L.R. Coelho, I. Keller, L.M. Dalcero, A.M. Rosa, C.A.R. Typing clinical and animal environment Aspergillus fumigatus gliotoxin producer strains isolated from Brazil by PCR-RFLP markers
topic_facet	Animal environment Aspergillus fumigatus Clinical (animal and human) Restriction enzymes Taxonomic state BccI enzyme gliotoxin MspI enzyme restriction endonuclease Sau3AI enzyme unclassified drug animal bioassay biomarker isolated population maize pathogen polymerase chain reaction sorghum taxonomy animal food article aspergillosis Aspergillus fumigatus bovine mastitis cereal computer model fungal strain fungus isolation maize morphology nucleotide sequence productivity restriction fragment length polymorphism reverse transcription polymerase chain reaction risk assessment rural population silage sorghum species identification Brazil Animalia Aspergillus fumigatus Bovinae Zea mays
description	Aspergillus fumigatus, a well-known human and animal pathogen causing aspergillosis, has been historically identified by morphological and microscopic features. However, recent studies have shown that species identification on the basis of morphology alone is problematic. The aim of this work was to confirm the taxonomic state at specie level of a set of clinical (human and animal) and animal environment A. fumigatus strains identified by morphological criteria applying a PCR-RFLP assay by an in silico and in situ analysis with three restriction enzymes. The A. fumigatus gliotoxin-producing ability was also determined. Previous to the in situ PCR-RFLP analysis, an in silico assay with BccI, MspI and Sau3AI restriction enzymes was carried out. After that, these enzymes were used for in situ assay. All A. fumigatus strains isolated from corn silage, human aspergillosis and bovine mastitis and high per cent of the strains isolated from cereals, animal feedstuff and sorghum silage were able to produce high gliotoxin levels. Also, all these strains identified by morphological criteria as A. fumigatus, regardless of its isolation source, had band patterns according to A. fumigatus sensu stricto by PCR-RFLP markers. © 2013 The Society for Applied Microbiology.
format	JOUR
author	Soleiro, C.A. Pena, G.A. Cavaglieri, L.R. Coelho, I. Keller, L.M. Dalcero, A.M. Rosa, C.A.R.
author_facet	Soleiro, C.A. Pena, G.A. Cavaglieri, L.R. Coelho, I. Keller, L.M. Dalcero, A.M. Rosa, C.A.R.
author_sort	Soleiro, C.A.
title	Typing clinical and animal environment Aspergillus fumigatus gliotoxin producer strains isolated from Brazil by PCR-RFLP markers
title_short	Typing clinical and animal environment Aspergillus fumigatus gliotoxin producer strains isolated from Brazil by PCR-RFLP markers
title_full	Typing clinical and animal environment Aspergillus fumigatus gliotoxin producer strains isolated from Brazil by PCR-RFLP markers
title_fullStr	Typing clinical and animal environment Aspergillus fumigatus gliotoxin producer strains isolated from Brazil by PCR-RFLP markers
title_full_unstemmed	Typing clinical and animal environment Aspergillus fumigatus gliotoxin producer strains isolated from Brazil by PCR-RFLP markers
title_sort	typing clinical and animal environment aspergillus fumigatus gliotoxin producer strains isolated from brazil by pcr-rflp markers
url	http://hdl.handle.net/20.500.12110/paper_02668254_v57_n6_p484_Soleiro
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_version_	1782029585583439872

Typing clinical and animal environment Aspergillus fumigatus gliotoxin producer strains isolated from Brazil by PCR-RFLP markers

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